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番茄Mi-1基因调控元件及同源基因进化分析 被引量:2
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作者 刘子记 刘维侠 +1 位作者 牛玉 杨衍 《江苏农业科学》 2020年第18期66-71,共6页
为阐明Mi-1基因转录调控元件及同源基因进化特点,从番茄品种京番308中克隆Mi-1基因起始密码子上游序列,选取转录起始位点上游1432 bp的序列进行启动子顺式调控元件分析,分析结果表明,Mi-1启动子除了含有核心元件外,还含有光响应元件、... 为阐明Mi-1基因转录调控元件及同源基因进化特点,从番茄品种京番308中克隆Mi-1基因起始密码子上游序列,选取转录起始位点上游1432 bp的序列进行启动子顺式调控元件分析,分析结果表明,Mi-1启动子除了含有核心元件外,还含有光响应元件、脱落酸响应元件、茉莉酸甲酯响应元件、低温响应元件、防御与胁迫响应元件等。Mi-1同源基因进化分析结果表明,Mi-1同源基因既包括旁系同源基因,也包括直系同源基因。当同源指数为0.70时,番茄、马铃薯、辣椒中含有Mi-1同源基因,与辣椒相比,番茄与马铃薯的亲缘关系更近。结果可为进一步研究Mi-1基因的调控表达和进化规律奠定基础。 展开更多
关键词 番茄 根结线虫抗性 启动子 同源基因 基因进化 顺式调控元件
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Resistance Evaluation of Capsicum spp. Germplasm to Meloidogyne incognita 被引量:3
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作者 刘子记 杜公福 +2 位作者 朱婕 申龙斌 曹振木 《Agricultural Science & Technology》 CAS 2016年第7期1723-1728,共6页
[Objective] This study aimed to screen out hot pepper germplasms highly resistant to Meloidogyne incognita, thereby providing resistant resources for hot pep- per breeding. [Method] Comprehensive analysis combining cl... [Objective] This study aimed to screen out hot pepper germplasms highly resistant to Meloidogyne incognita, thereby providing resistant resources for hot pep- per breeding. [Method] Comprehensive analysis combining cluster analysis and sub- ordinate function was conducted through determining related resistance indexes of 67 hot pepper germplasms 50 days after inoculated with M. incognita. [Result] The effects of M. incognita on related resistance indexes were significantly different am- ong the hot pepper germplasms. Egg index and gall index had abundant genetic variation with variation coefficients of 143.16% and 118.95%, respectively. Based on the gall indexes, cluster analysis of hot pepper germplasms was performed. The 67 hot pepper germplasms were divided into 4 groups (resistant, moderately resistant, susceptible and high susceptible). The resistance intensity of the hot pepper germplasms were ranked according to the sum of subordinate function values of various resistance indexes. The total function values of Rela 2 and L506M were the largest (2.00), indicating that these two germplasms were immune to M. incognita. The total function values of L287-2, L522-1M, L504M, L515-2, 13SM100-1, L512M, L292-1, L319, L316, L317, 13SM87-1 and Rela 5 were larger than 1.95, indicating that these germplasms were highly resistant to M. incognita. [Conclusion] This study could provide certain resistant resources for resistance breeding of hot pepper to M. incognita. 展开更多
关键词 Capsicum spp. Gerrnplasm Meloidogyne incognita Resistance index Cluster analysis Subordinate function analysis
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Screening of genotypes for their resistance againstMeloidogyne incognita and Fusarium chlamydosporum causing wilt complex on Coleusforskohlii Briq.
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作者 Devappa V. Kumar B. +5 位作者 Gangadhara Naik. Nagaraja.R. Ravindra H. Kamanna B.C. Jahagirdar.S. L.Hegde 《Journal of Agricultural Science and Technology》 2009年第12期16-18,共3页
A pot culture experiment was carried out for the Screening of 11 popular genotypes against to Meloidogyne incognita and Fusarium chlamydosporum wilt complex on Coleus forskohlii. Genotype viz., yellow tubers and Rabak... A pot culture experiment was carried out for the Screening of 11 popular genotypes against to Meloidogyne incognita and Fusarium chlamydosporum wilt complex on Coleus forskohlii. Genotype viz., yellow tubers and Rabakavi local showed resistant reaction, Orange tuber and Nimbanur local showed moderately resistant reaction. Rest of the seven genotvDes showed suscentible reactions. 展开更多
关键词 Coleus genotypes Meloidogyne incognita Fusarium chlamydosporum Coleus forskohlii
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