The potential influences of cadmium (Cd) on the biochemical processes of the soil nitrogen (N) cycle, along with the dynamics of ammonification, nitrification, and denitrification processes in the rhizosphere and non-...The potential influences of cadmium (Cd) on the biochemical processes of the soil nitrogen (N) cycle, along with the dynamics of ammonification, nitrification, and denitrification processes in the rhizosphere and non-rhizosphere (bulk soil), respectively, were investigated in a Cd-stressed system during an entire soybean growing season. In terms of Cd pollution at the seedling stage, the ammonifying bacteria proved to be the most sensitive microorganisms, whereas the effects of Cd on denitrification were not obvious. Following the growth of soybeans, the influences of Cd on ammonification in the bulk soil were: toxic impacts at the seedling stage, stimulatory effects during the early flowering stage, and adaptation to the pollutant during the podding and ripening stages. Although nitrification and denitrification in the bulk soil decreased throughout the entire growth cycle, positive adaptation to Cd stress was observed during the ripening stage. Moreover, during the ripening stage, denitrification in the bulk soil under high Cd treatment (20 mg kg-1) was even higher than that in the control, indicating a probable change in the ecology of the denitrifying microbes in the Cd-stressed system. Changes in the activity of microbes in the rhizosphere following plant growth were similar to those in the non-rhizosphere in Cd treatments; however, the tendency of change in the rhizosphere seemed to be more moderate. This suggested that there was some mitigation of Cd stress in the rhizosphere.展开更多
The vegetation and soil are mutual environmental factors, soil characteristics, such as chemical properties and microorganism that affect the vegetation occurrence, development and succession speed. In this study, we ...The vegetation and soil are mutual environmental factors, soil characteristics, such as chemical properties and microorganism that affect the vegetation occurrence, development and succession speed. In this study, we evaluated the structure of microbial communities of rhizosphere of Cowskin Azalea(Rhododendron aureum Georgi) populations and compared with non-rhizosphere soils at four sample sites of the Changbai Mountains, China, and analyzed the correlation between chemical properties of soil and microbial communities. The results showed that microbial structure and soil chemical properties are significant superior to non-rhizosphere at all four sample sites. The rhizosphere microorganisms are mainly composed of bacteria, actinomycetes, followed by fungi least. The principal component analysis(PCA) biplot displayed that there are differences between rhizosphere and non-rhizosphere soils for microflora; Through correlation analysis, we found that the bacteria is clearly influenced by p H on the Changbai Mountains, besides p H, other soil features such as NO3–-N. These data highlight that R. aureum as the dominant vegetation living in the alpine tundra is a key factor in the formation of soil microorganism and improving soil fertility, and is of great significance for the maintenance of alpine tundra ecosystem.展开更多
Phosphate solubilizing bacteria isolated from rhizosphere of coffee plants may play an important role in improving phosphate availability for the plants. However, one of the factors influencing the degree of phosphate...Phosphate solubilizing bacteria isolated from rhizosphere of coffee plants may play an important role in improving phosphate availability for the plants. However, one of the factors influencing the degree of phosphate solubilization by these bacteria is the ability of the microorganisms to utilize phosphate. The objective of this study was to determine the ability of phosphate solubilizing bacteria isolated from coffee plant rhizosphere and their effects on robusta coffee seedling growth. This research was carried out by taking soil samples from Andungsari (Bondowoso District) and Kaliwining (Jember District) coffee plantations, both located in East Java. Liquid medium of Pikovskaya was used for isolation of phosphate solubilizing bacteria from the soil samples. Results of this study showed that 12 phosphate solubilizing bacteria were obtained from this isolation, eight isolates from Andungsari and four isolates from Kaliwining. Selection of those bacteria isolates was based on the qualitative ability in phosphate solubilizing by measuring the clear zone surrounding the colonies and quantitatively by measuring the solubilized phosphate using spectrophotometer. The results showed that four isolates, in the order of PFpKW1, PFpC61, PFsC62a, and PFsB 11, had the highest qualitative ability in solubilizing phosphate, while for the highest quantitative ability the order was PFpKW 1, PFpC61, PFsC62a, and PFsB 11. In a green house study, inoculation of these selected isolates onto Robusta coffee seedlings positively enhanced the coffee seedling growth. Phenotypic test indicated that the four isolates are similar to the genus of Pseudomonas.展开更多
The disadvantages of the long term application of Tilemsi natural phosphate (TNP) on maize (Zea mays L.) production has come out because of its low P availability. Some functional soil microbes, such as phosphate ...The disadvantages of the long term application of Tilemsi natural phosphate (TNP) on maize (Zea mays L.) production has come out because of its low P availability. Some functional soil microbes, such as phosphate dissolving bacteria, have great potential in improvement of P solubility from TNP and P uptake by plants. The present study aimed to isolate and characterize typical phosphate dissolving bacterial strains (Bacillus subtilis) from Malian soils, and investigate their role in P uptake by maize grown in soils amended with TNP. The experimental design was a split plot with three main plots of fertilizers sources, i.e., natural phosphate, commercial fertilizer and without fertilizer, and with seven sub-plots of six microorganisms plus the control. The field experiment results have shown that the maize inoculated with the phosphate dissolving bacteria was improved in seed germination, plant growth, plant production (increase yield by 42%), grain and aerial dry biomass (P) content of 34% and 64%, respectively. They have also shown that the locally available TNP can be used by the Malians farmers in maize culture and have comparable production to the one obtained with the costly imported commercial phosphate fertilizer, like the complex cereal. The project has provided information for the combined use of the Mali TNP and phosphate dissolving bacteria Bacillus subtilis subsp, subtilis (T): DSM 10 in improvement of maize production in the country.展开更多
The vast majority of herbaceous plants engage into arbuscular mycorrhizal (AM) symbioses and consideration of their mycorrhizal status should be embodied in studies of plant-microbe interactions. To establish reliable...The vast majority of herbaceous plants engage into arbuscular mycorrhizal (AM) symbioses and consideration of their mycorrhizal status should be embodied in studies of plant-microbe interactions. To establish reliable AM contrasts, however, a sterilized re-inoculation procedure is commonly adopted. It was questioned whether the specific approach is sufficient for the studies targeting the bacterial domain, specifically nitrifiers, a group of autotrophic, slow growing microbes. In a controlled experiment mycorrhizal and non-mycorrhizal Plantago lanceolata were grown up in compartmentalized pots to study the AM effect on nitrification rates in the plant rhizosphere. Nitrification rates were assayed following an extensive 3-week bacterial equilibration step of the re-inoculated soil and a 13-week plant growth period in a controlled environment. Under these specific conditions, the nitrification potential levels at harvest were exceptionally low, and actual nitrification rates of the root compartment of non-mycorrhizal P. lanceolata were significantly lower than those of any other compartment. It is then argued that the specific effects should be attributed to the alleged higher growth rates of non-mycorrhizal plants that are known to occur early in the AM experiment. It is concluded that the specific experimental approach is not suitable for the study of microbes with slow growth rates.展开更多
Plant growth-promoting rhizobacteria (PGPR) have been widely recognized as an important agent, especially as a biofertilizer, in agricultural systems. The objectives of this study were to select effective PGPR for C...Plant growth-promoting rhizobacteria (PGPR) have been widely recognized as an important agent, especially as a biofertilizer, in agricultural systems. The objectives of this study were to select effective PGPR for Chinese kale (Brassica oleracea var. alboglabra) cultivation and to investigate the effect of their inoculation on indigenous microbial community structure. The Bacillus sp. SUT1 and Pseudomonas sp. SUT19 were selected for determining the efficiency in promoting Chinese kale growth in both pot and field experiments. In the field experiment, PGPR amended with compost gave the highest yields among all treatments. The Chinese kale growth promotion may be directly affected by PGPR inoculation. The changes of microbial community structure in the rhizosphere of Chinese kale following PGPR inoculation were examined by denaturing gradient gel electrophoresis (DGGE) and principal coordinate analysis. The DGGE fingerprints of 16S rDNA amplified from total community DNA in the rhizosphere confirmed that our isolates were established in the rhizosphere throughout this study. The microbial community structures were slightly different among all the treatments, and the major changes depended on stages of plant growth. DNA sequencing of excised DGGE bands showed that the dominant species in microbial community structure in the rhizosphere were not mainly interfered by PGPR, but strongly influenced by plant development. The microbial diversity as revealed by diversity indices was not different between the PGPR-inoculated and uninoculated treatments. In addition, the rhizosphere soil had more influence on eubacterial diversity, whereas it did not affect archaebacterial and fungal diversities.展开更多
Phytoremediation is an emerging technology that uses plants and their associated microbes to clean up pollutants from the soil, water, and air. In order to select the plant growth-promoting rhizobacteria (PGPR) for ...Phytoremediation is an emerging technology that uses plants and their associated microbes to clean up pollutants from the soil, water, and air. In order to select the plant growth-promoting rhizobacteria (PGPR) for phytoremediation of heavy metal contamination, 60 bacterial strains were isolated from the rhizosphere of two endemic plants, Prosopis laevigata and Spharealcea angustifolia, in a heavy metal-contaminated zone in Mexico. These rhizobacterial strains were characterized for the growth at different pH and salinity, extracellular enzyme production, solubilization of phosphate, heavy metal resistance, and plant growth-promoting (PGP) traits, including production of siderophores and indol-3-acetic acid (IAA). Overall, the obtained rhizobacteria presented multiple PGP traits. These rhizoba^teria were also resistant to high levels of heavy metals (including As as a metalloid) (up to 480 mmol L-1 As(V), 24 mmol L-1 Pb(II), 21 mmol L-1 Cu(II), and 4.5 mmol L-1 Zn(II)). Seven rhizobacterial strains with the best PGP traits were identified as members of Alcaligenes, Bacillus, Curtobacterium, and Microbacterium, and were selected for further bioassay. The inoculation of Brassica nigra seeds with Microbacteriurn sp. CE3R2, Microbacterium sp. NE1R5, Curtobacterium sp. NM1R1, and Microbacterium sp. NM3E9 facilitated the root development; they significantly improved the B. nigra seed germination and root growth in the presence of heavy metals such as 2.2 mmol L-1 Zn(II). The rhizobacterial strains isolated in the present study had the potential to be used as efficient bioinoculants in phytorremediation of soils contaminated with multiple heavy metals.展开更多
Application of plant growth-promoting rhizobacteria (PGPR) has been shown to increase legume growth and development under field and controlled environmental conditions. The present study was conducted to isolate pla...Application of plant growth-promoting rhizobacteria (PGPR) has been shown to increase legume growth and development under field and controlled environmental conditions. The present study was conducted to isolate plant growth-promoting rhizobacteria (PGPR) from the root nodules of lentil (Lens culinaris Medik.) grown in arid/semi-arid region of Punjab, Pakistan and examined their plant growth-promoting abilities. Five bacterial isolates were isolated, screened in vitro for plant growth-promoting (PGP) characteristics and their effects on the growth of lentil were assessed under in vitro, hydroponic and greenhouse (pot experiment) conditions. All the isolates were Gram negative, rod-shaped and circular in form and exhibited the plant growth-promoting attributes of phosphate solubilization and auxin (indole acetic acid, IAA) production. The IAA production capacity ranged in 0.5-11.0μg mL-1 and P solubilization ranged in 3-16 mg L-1. When tested for their effects on plant growth, the isolated strains had a stimulatory effect on growth, nodulation and nitrogen (N) and phosphorus (P) uptake in plants on nutrient-deficient soil. In the greenhouse pot experiment, application of PGPR significantly increased shoot length, fresh weight and dry weight by 65%, 43% and 63% and the increases in root length, fresh weight and dry weight were 74%, 54% and 92%, respectively, as compared with the uninoculated control. The relative increases in growth characteristics under in vitro and hydroponic conditions were even higher. PGPR also increased the number of pods per plant, 1 000-grain weight, dry matter yield and grain yield by 50%, 13%, 2870 and 29%, respectively, over the control. The number of nodules and nodule dry mass increased by 170% and 136%, respectively. After inoculation with effective bacterial strains, the shoot, root and seed N and P contents increased, thereby increasing both N and P uptake in plants. The root elongation showed a positive correlation (R2 = 0.67) with the IAA production and seed yield exhibited a positive correlation (R2 = 0.82) with root nodulation. These indicated that the isolated PGPR rhizobial strains can be best utilized as potential agents or biofertilizers for stimulating the growth and nutrient accumulation of lentil.展开更多
The effect of exotic plants on Bacillus diversity in the rhizosphere and the role of Bacilli in exotic or native plant species remain poorly understood.Flaveria bidentis is an invasive grass in China.Setaria viridis i...The effect of exotic plants on Bacillus diversity in the rhizosphere and the role of Bacilli in exotic or native plant species remain poorly understood.Flaveria bidentis is an invasive grass in China.Setaria viridis is a native grass and occurs in areas invaded by F.bidentis.Our objectives were(i)to examine the differences in the Bacillus communities between F.bidentis and S.viridis rhizospheres soil,and(ii)to compare the effects of Bacilli from F.bidentis and S.viridis rhizospheres on the competitiveness of the invasive species.Flaveria bidentis monoculture,mixture of F.bidentis and S.viridis and S.viridis monoculture were designed in the field experiment.Bacillus diversity in their rhizosphere was analyzed using 16S rRNA.One of the dominant Bacilli in the rhizosphere soil of F.bidentis was selected to test its effect on the competitive growth of F.bidentis in a greenhouse experiment.Bacillus diversity differed in F.bidentis and S.viridis rhizosphere.Brevibacterium frigoritolerans was the dominant Bacilli in the rhizosphere of both F.bidentis and S.viridis;however,its relative abundance in the F.bidentis rhizosphere was much higher than that in the S.viridis rhizosphere.In addition,B.frigoritolerans in the F.bidentis rhizosphere enhanced the growth of the plant compared with that of S.viridis by improving the nitrogen and phosphorus levels.This study showed that F.bidentis invasion influenced Bacillus communities,especially B.frigoritolerans,which,in turn,facilitated F.bidentis growth by increasing the levels of available nitrogen and phosphorus.展开更多
基金Project supported by the National Natural Science Foundation of China (No. 2977020)the National Key Basic Research Program (973 Program) of China (No. 2002CB410807)
文摘The potential influences of cadmium (Cd) on the biochemical processes of the soil nitrogen (N) cycle, along with the dynamics of ammonification, nitrification, and denitrification processes in the rhizosphere and non-rhizosphere (bulk soil), respectively, were investigated in a Cd-stressed system during an entire soybean growing season. In terms of Cd pollution at the seedling stage, the ammonifying bacteria proved to be the most sensitive microorganisms, whereas the effects of Cd on denitrification were not obvious. Following the growth of soybeans, the influences of Cd on ammonification in the bulk soil were: toxic impacts at the seedling stage, stimulatory effects during the early flowering stage, and adaptation to the pollutant during the podding and ripening stages. Although nitrification and denitrification in the bulk soil decreased throughout the entire growth cycle, positive adaptation to Cd stress was observed during the ripening stage. Moreover, during the ripening stage, denitrification in the bulk soil under high Cd treatment (20 mg kg-1) was even higher than that in the control, indicating a probable change in the ecology of the denitrifying microbes in the Cd-stressed system. Changes in the activity of microbes in the rhizosphere following plant growth were similar to those in the non-rhizosphere in Cd treatments; however, the tendency of change in the rhizosphere seemed to be more moderate. This suggested that there was some mitigation of Cd stress in the rhizosphere.
基金Wildlife Conservation and Management of National Forestry Bureau of China
文摘The vegetation and soil are mutual environmental factors, soil characteristics, such as chemical properties and microorganism that affect the vegetation occurrence, development and succession speed. In this study, we evaluated the structure of microbial communities of rhizosphere of Cowskin Azalea(Rhododendron aureum Georgi) populations and compared with non-rhizosphere soils at four sample sites of the Changbai Mountains, China, and analyzed the correlation between chemical properties of soil and microbial communities. The results showed that microbial structure and soil chemical properties are significant superior to non-rhizosphere at all four sample sites. The rhizosphere microorganisms are mainly composed of bacteria, actinomycetes, followed by fungi least. The principal component analysis(PCA) biplot displayed that there are differences between rhizosphere and non-rhizosphere soils for microflora; Through correlation analysis, we found that the bacteria is clearly influenced by p H on the Changbai Mountains, besides p H, other soil features such as NO3–-N. These data highlight that R. aureum as the dominant vegetation living in the alpine tundra is a key factor in the formation of soil microorganism and improving soil fertility, and is of great significance for the maintenance of alpine tundra ecosystem.
文摘Phosphate solubilizing bacteria isolated from rhizosphere of coffee plants may play an important role in improving phosphate availability for the plants. However, one of the factors influencing the degree of phosphate solubilization by these bacteria is the ability of the microorganisms to utilize phosphate. The objective of this study was to determine the ability of phosphate solubilizing bacteria isolated from coffee plant rhizosphere and their effects on robusta coffee seedling growth. This research was carried out by taking soil samples from Andungsari (Bondowoso District) and Kaliwining (Jember District) coffee plantations, both located in East Java. Liquid medium of Pikovskaya was used for isolation of phosphate solubilizing bacteria from the soil samples. Results of this study showed that 12 phosphate solubilizing bacteria were obtained from this isolation, eight isolates from Andungsari and four isolates from Kaliwining. Selection of those bacteria isolates was based on the qualitative ability in phosphate solubilizing by measuring the clear zone surrounding the colonies and quantitatively by measuring the solubilized phosphate using spectrophotometer. The results showed that four isolates, in the order of PFpKW1, PFpC61, PFsC62a, and PFsB 11, had the highest qualitative ability in solubilizing phosphate, while for the highest quantitative ability the order was PFpKW 1, PFpC61, PFsC62a, and PFsB 11. In a green house study, inoculation of these selected isolates onto Robusta coffee seedlings positively enhanced the coffee seedling growth. Phenotypic test indicated that the four isolates are similar to the genus of Pseudomonas.
文摘The disadvantages of the long term application of Tilemsi natural phosphate (TNP) on maize (Zea mays L.) production has come out because of its low P availability. Some functional soil microbes, such as phosphate dissolving bacteria, have great potential in improvement of P solubility from TNP and P uptake by plants. The present study aimed to isolate and characterize typical phosphate dissolving bacterial strains (Bacillus subtilis) from Malian soils, and investigate their role in P uptake by maize grown in soils amended with TNP. The experimental design was a split plot with three main plots of fertilizers sources, i.e., natural phosphate, commercial fertilizer and without fertilizer, and with seven sub-plots of six microorganisms plus the control. The field experiment results have shown that the maize inoculated with the phosphate dissolving bacteria was improved in seed germination, plant growth, plant production (increase yield by 42%), grain and aerial dry biomass (P) content of 34% and 64%, respectively. They have also shown that the locally available TNP can be used by the Malians farmers in maize culture and have comparable production to the one obtained with the costly imported commercial phosphate fertilizer, like the complex cereal. The project has provided information for the combined use of the Mali TNP and phosphate dissolving bacteria Bacillus subtilis subsp, subtilis (T): DSM 10 in improvement of maize production in the country.
基金Supported by a PhD fellowship from the Chloros trust
文摘The vast majority of herbaceous plants engage into arbuscular mycorrhizal (AM) symbioses and consideration of their mycorrhizal status should be embodied in studies of plant-microbe interactions. To establish reliable AM contrasts, however, a sterilized re-inoculation procedure is commonly adopted. It was questioned whether the specific approach is sufficient for the studies targeting the bacterial domain, specifically nitrifiers, a group of autotrophic, slow growing microbes. In a controlled experiment mycorrhizal and non-mycorrhizal Plantago lanceolata were grown up in compartmentalized pots to study the AM effect on nitrification rates in the plant rhizosphere. Nitrification rates were assayed following an extensive 3-week bacterial equilibration step of the re-inoculated soil and a 13-week plant growth period in a controlled environment. Under these specific conditions, the nitrification potential levels at harvest were exceptionally low, and actual nitrification rates of the root compartment of non-mycorrhizal P. lanceolata were significantly lower than those of any other compartment. It is then argued that the specific effects should be attributed to the alleged higher growth rates of non-mycorrhizal plants that are known to occur early in the AM experiment. It is concluded that the specific experimental approach is not suitable for the study of microbes with slow growth rates.
基金Supported by the Suranaree University of Technology and the Higher Education Research Promotionthe National Research University Project of Thailand,Office of the Higher Education Commission
文摘Plant growth-promoting rhizobacteria (PGPR) have been widely recognized as an important agent, especially as a biofertilizer, in agricultural systems. The objectives of this study were to select effective PGPR for Chinese kale (Brassica oleracea var. alboglabra) cultivation and to investigate the effect of their inoculation on indigenous microbial community structure. The Bacillus sp. SUT1 and Pseudomonas sp. SUT19 were selected for determining the efficiency in promoting Chinese kale growth in both pot and field experiments. In the field experiment, PGPR amended with compost gave the highest yields among all treatments. The Chinese kale growth promotion may be directly affected by PGPR inoculation. The changes of microbial community structure in the rhizosphere of Chinese kale following PGPR inoculation were examined by denaturing gradient gel electrophoresis (DGGE) and principal coordinate analysis. The DGGE fingerprints of 16S rDNA amplified from total community DNA in the rhizosphere confirmed that our isolates were established in the rhizosphere throughout this study. The microbial community structures were slightly different among all the treatments, and the major changes depended on stages of plant growth. DNA sequencing of excised DGGE bands showed that the dominant species in microbial community structure in the rhizosphere were not mainly interfered by PGPR, but strongly influenced by plant development. The microbial diversity as revealed by diversity indices was not different between the PGPR-inoculated and uninoculated treatments. In addition, the rhizosphere soil had more influence on eubacterial diversity, whereas it did not affect archaebacterial and fungal diversities.
基金supported by the Secretaría de Investigación y Posgrado-Insituto Politécnico Nacional (IPN), México (No. 20130722)the fellowships provided by Consejo Nacional de Ciencia y Tecnología (CONACYT), México+1 种基金by Becas de Estímulo Institucional de Formación de Investigadores-IPN, Méxicothe scholarships of Comisión de Operación y Fomentode Actividades Académicas and Estímulos al Desempeo de los Investigadores-IPN and Sistema Nacional de Investigadores-CONACYT, México
文摘Phytoremediation is an emerging technology that uses plants and their associated microbes to clean up pollutants from the soil, water, and air. In order to select the plant growth-promoting rhizobacteria (PGPR) for phytoremediation of heavy metal contamination, 60 bacterial strains were isolated from the rhizosphere of two endemic plants, Prosopis laevigata and Spharealcea angustifolia, in a heavy metal-contaminated zone in Mexico. These rhizobacterial strains were characterized for the growth at different pH and salinity, extracellular enzyme production, solubilization of phosphate, heavy metal resistance, and plant growth-promoting (PGP) traits, including production of siderophores and indol-3-acetic acid (IAA). Overall, the obtained rhizobacteria presented multiple PGP traits. These rhizoba^teria were also resistant to high levels of heavy metals (including As as a metalloid) (up to 480 mmol L-1 As(V), 24 mmol L-1 Pb(II), 21 mmol L-1 Cu(II), and 4.5 mmol L-1 Zn(II)). Seven rhizobacterial strains with the best PGP traits were identified as members of Alcaligenes, Bacillus, Curtobacterium, and Microbacterium, and were selected for further bioassay. The inoculation of Brassica nigra seeds with Microbacteriurn sp. CE3R2, Microbacterium sp. NE1R5, Curtobacterium sp. NM1R1, and Microbacterium sp. NM3E9 facilitated the root development; they significantly improved the B. nigra seed germination and root growth in the presence of heavy metals such as 2.2 mmol L-1 Zn(II). The rhizobacterial strains isolated in the present study had the potential to be used as efficient bioinoculants in phytorremediation of soils contaminated with multiple heavy metals.
基金Supported by the University of Azad Jammu and Kashmir, Pakistan and the Pakistan Agriculture Research Council, Pakistan (No. ALP NR-27)
文摘Application of plant growth-promoting rhizobacteria (PGPR) has been shown to increase legume growth and development under field and controlled environmental conditions. The present study was conducted to isolate plant growth-promoting rhizobacteria (PGPR) from the root nodules of lentil (Lens culinaris Medik.) grown in arid/semi-arid region of Punjab, Pakistan and examined their plant growth-promoting abilities. Five bacterial isolates were isolated, screened in vitro for plant growth-promoting (PGP) characteristics and their effects on the growth of lentil were assessed under in vitro, hydroponic and greenhouse (pot experiment) conditions. All the isolates were Gram negative, rod-shaped and circular in form and exhibited the plant growth-promoting attributes of phosphate solubilization and auxin (indole acetic acid, IAA) production. The IAA production capacity ranged in 0.5-11.0μg mL-1 and P solubilization ranged in 3-16 mg L-1. When tested for their effects on plant growth, the isolated strains had a stimulatory effect on growth, nodulation and nitrogen (N) and phosphorus (P) uptake in plants on nutrient-deficient soil. In the greenhouse pot experiment, application of PGPR significantly increased shoot length, fresh weight and dry weight by 65%, 43% and 63% and the increases in root length, fresh weight and dry weight were 74%, 54% and 92%, respectively, as compared with the uninoculated control. The relative increases in growth characteristics under in vitro and hydroponic conditions were even higher. PGPR also increased the number of pods per plant, 1 000-grain weight, dry matter yield and grain yield by 50%, 13%, 2870 and 29%, respectively, over the control. The number of nodules and nodule dry mass increased by 170% and 136%, respectively. After inoculation with effective bacterial strains, the shoot, root and seed N and P contents increased, thereby increasing both N and P uptake in plants. The root elongation showed a positive correlation (R2 = 0.67) with the IAA production and seed yield exhibited a positive correlation (R2 = 0.82) with root nodulation. These indicated that the isolated PGPR rhizobial strains can be best utilized as potential agents or biofertilizers for stimulating the growth and nutrient accumulation of lentil.
基金funded by the National Natural Science Foundation of China(31972343)Hebei National Natural Science Foundation(C2019201059)College of Life Science,Institute of Life Science and Green Development,Hebei University.
文摘The effect of exotic plants on Bacillus diversity in the rhizosphere and the role of Bacilli in exotic or native plant species remain poorly understood.Flaveria bidentis is an invasive grass in China.Setaria viridis is a native grass and occurs in areas invaded by F.bidentis.Our objectives were(i)to examine the differences in the Bacillus communities between F.bidentis and S.viridis rhizospheres soil,and(ii)to compare the effects of Bacilli from F.bidentis and S.viridis rhizospheres on the competitiveness of the invasive species.Flaveria bidentis monoculture,mixture of F.bidentis and S.viridis and S.viridis monoculture were designed in the field experiment.Bacillus diversity in their rhizosphere was analyzed using 16S rRNA.One of the dominant Bacilli in the rhizosphere soil of F.bidentis was selected to test its effect on the competitive growth of F.bidentis in a greenhouse experiment.Bacillus diversity differed in F.bidentis and S.viridis rhizosphere.Brevibacterium frigoritolerans was the dominant Bacilli in the rhizosphere of both F.bidentis and S.viridis;however,its relative abundance in the F.bidentis rhizosphere was much higher than that in the S.viridis rhizosphere.In addition,B.frigoritolerans in the F.bidentis rhizosphere enhanced the growth of the plant compared with that of S.viridis by improving the nitrogen and phosphorus levels.This study showed that F.bidentis invasion influenced Bacillus communities,especially B.frigoritolerans,which,in turn,facilitated F.bidentis growth by increasing the levels of available nitrogen and phosphorus.