To overcome the issues of high cost and continuous cropping obstacles in facility cultivation of Panax notoginseng_ F. H. Chen, satisfy the market demand, save the production cost, improve the utilization rate of fore...To overcome the issues of high cost and continuous cropping obstacles in facility cultivation of Panax notoginseng_ F. H. Chen, satisfy the market demand, save the production cost, improve the utilization rate of forest land, increase the in-come of forest farmers and protect the ecological environment, the cultivation tech-niques of high-quality P. notoginseng seedlings from Wenshan, Yunnan under four kinds of forests （walnut forest, China fir forest, grape forest and kiwi forest） were in-vestigated in this study. The results showed that the height growth, crown diameter, survival rate and 3-year-old tuber weight of P. notoginseng_under walnut forest were higher than those under the other three kinds of forests; the height growth, crown diameter, survival rate and 3-year-old tuber weight of P. notoginseng under China fir forest were higher than those under grape forest and kiwi forest; and the crown di-ameter and survival rate under grape forest were higher, and the height growth and tuber weight under grape forest were lower than those under kiwi forest. Walnut is a broad-leaved deciduous tree species, so large-scale cultivation of P. notoginseng_should be conducted under broadleaf deciduous forest with canopy density around 0.8, taking advantage of the cool environment and rich humus layer under forest. This cultivation technology could save labor, shade, fertilizer and other costs, and accord with the ecological habit and the growth rules of P. notoginseng, thus im-proving yield and achieving high economic benefit.展开更多
This study was conducted for the purposes of reducing consumption of wild resources of Paris polyphylla Smith, satisfying the market demand, saving the production cost, improving utilization rate of forest land, incre...This study was conducted for the purposes of reducing consumption of wild resources of Paris polyphylla Smith, satisfying the market demand, saving the production cost, improving utilization rate of forest land, increasing income of foresters and protecting ecological environment. Three types of fores{ land, i.e., the evergreen forest land （China fir forest）, deciduous forest land （walnut forest） and shrub land （honey suckle forest.） were selected, and seeds and cut rhizomes of Paris polyphylla Smith produced in Bamian Mountain, Hunan, were used in the study on understory seedling raising and cultivation techniques. The results showed that： when using different propagation materials, seed propagation could be adopted to reduce the consumption of wild resources and provide a large number of seedlings, and was beneficial to large-scale planting; and when conducing seedling and plantlet raising followed by transplanting and direct seeding and planting using different propagation materials in large-scale understory cultivation, direct seeding/ planting was appropriate for saving save production cost with the advantage of early production. Among the 3 different types of forest land, the best choice was broad- leaved forest land, which could achieve better economic benefit because of con- forming with the ecological habit and growth and development law of Paris poly- phylla Smith.展开更多
Conversion of natural forests into pure plantation forests is a common management practice in subtropical China.To evaluate the effects of forest conversion on soil fertility, microbe numbers and enzyme activities in ...Conversion of natural forests into pure plantation forests is a common management practice in subtropical China.To evaluate the effects of forest conversion on soil fertility, microbe numbers and enzyme activities in topsoils (0-10 cm)were quantified in two 33-year-old monoculture plantations of Castanopsis kawakamii Hayata (CK) and Cunninghamia lanceolata Lamb. (Chinese fir) (CF), and compared to a neighboring relict natural C. kawakamii forest (NF), in Sanming,Fujian. Five soil samples were collected once each in January, April, July, September and November in 2000 in each forest for laboratory analysis. Over the sampling year, there were significant differences for bacteria, fungi and actinomycetes between forests and between seasons (P ＜ 0.05). The largest bacteria and fungi populations were in NF, while CF contained the greatest number of actinomycetes. There were also significant differences (P ＜ 0.05) with microbial respiration for forests and seasons. Additionally, compared with NF, urease and acid phosphatase were significantly lower (P ＜ 0.05)in CK and CF. Also, the correlations of soil hydrolysable N and available P to soil microbial and enzymatic activities were highly significant (P ＜ 0.01). Thus, to alter the traditional Chinese fir monoculture so as to mimic the natural forest conditions, managing mixed stands of Chinese fir and broadleaf trees or conducting crop rotation of conifers and broadleaf trees as well as minimizing forest disturbances like clear-cutting, slash burning and soil preparing, could be utilized.展开更多
UGPase gene related with wood cellulose synthesis was transferred into C. acuminata using the method of Agrobacte- rium-mediated genetic transformation, and an efficient transformation system was developed for C. acum...UGPase gene related with wood cellulose synthesis was transferred into C. acuminata using the method of Agrobacte- rium-mediated genetic transformation, and an efficient transformation system was developed for C. acuminata on the basis of evaluations of several factors affecting Agrobacterium-mediated DNA transfer rate. The highest transformation rate was achieved when pre-cultttred leaf explants were infected with an Agrobacterium culture corresponding to OD600 （0.5） for 10 min, and cultured on explant regeneration medium for three days. The results of Southern hybridization showed that genomic DNA of the kanamycin-resistant shoots to an UGPase gene probe substantiated the integration of the transgene. Transformation efficiency （6%） was achieved under the optimized transformation procedure, This system should facilitate the introduction of important useful genes into C, acuminata.展开更多
基金Supported by Central Financial Forestry Science and Technology Extension Project of China([2016]XT001)Science and Technology Development Project of Hunan Province(S2014F209021)~~
文摘To overcome the issues of high cost and continuous cropping obstacles in facility cultivation of Panax notoginseng_ F. H. Chen, satisfy the market demand, save the production cost, improve the utilization rate of forest land, increase the in-come of forest farmers and protect the ecological environment, the cultivation tech-niques of high-quality P. notoginseng seedlings from Wenshan, Yunnan under four kinds of forests （walnut forest, China fir forest, grape forest and kiwi forest） were in-vestigated in this study. The results showed that the height growth, crown diameter, survival rate and 3-year-old tuber weight of P. notoginseng_under walnut forest were higher than those under the other three kinds of forests; the height growth, crown diameter, survival rate and 3-year-old tuber weight of P. notoginseng under China fir forest were higher than those under grape forest and kiwi forest; and the crown di-ameter and survival rate under grape forest were higher, and the height growth and tuber weight under grape forest were lower than those under kiwi forest. Walnut is a broad-leaved deciduous tree species, so large-scale cultivation of P. notoginseng_should be conducted under broadleaf deciduous forest with canopy density around 0.8, taking advantage of the cool environment and rich humus layer under forest. This cultivation technology could save labor, shade, fertilizer and other costs, and accord with the ecological habit and the growth rules of P. notoginseng, thus im-proving yield and achieving high economic benefit.
基金Supported by Central Financial Forestry Science and Technology Promotion project([2014]XT003)~~
文摘This study was conducted for the purposes of reducing consumption of wild resources of Paris polyphylla Smith, satisfying the market demand, saving the production cost, improving utilization rate of forest land, increasing income of foresters and protecting ecological environment. Three types of fores{ land, i.e., the evergreen forest land （China fir forest）, deciduous forest land （walnut forest） and shrub land （honey suckle forest.） were selected, and seeds and cut rhizomes of Paris polyphylla Smith produced in Bamian Mountain, Hunan, were used in the study on understory seedling raising and cultivation techniques. The results showed that： when using different propagation materials, seed propagation could be adopted to reduce the consumption of wild resources and provide a large number of seedlings, and was beneficial to large-scale planting; and when conducing seedling and plantlet raising followed by transplanting and direct seeding and planting using different propagation materials in large-scale understory cultivation, direct seeding/ planting was appropriate for saving save production cost with the advantage of early production. Among the 3 different types of forest land, the best choice was broad- leaved forest land, which could achieve better economic benefit because of con- forming with the ecological habit and growth and development law of Paris poly- phylla Smith.
基金the Basic Research Program of Fujian Province (No. 2000-F-004).
文摘Conversion of natural forests into pure plantation forests is a common management practice in subtropical China.To evaluate the effects of forest conversion on soil fertility, microbe numbers and enzyme activities in topsoils (0-10 cm)were quantified in two 33-year-old monoculture plantations of Castanopsis kawakamii Hayata (CK) and Cunninghamia lanceolata Lamb. (Chinese fir) (CF), and compared to a neighboring relict natural C. kawakamii forest (NF), in Sanming,Fujian. Five soil samples were collected once each in January, April, July, September and November in 2000 in each forest for laboratory analysis. Over the sampling year, there were significant differences for bacteria, fungi and actinomycetes between forests and between seasons (P ＜ 0.05). The largest bacteria and fungi populations were in NF, while CF contained the greatest number of actinomycetes. There were also significant differences (P ＜ 0.05) with microbial respiration for forests and seasons. Additionally, compared with NF, urease and acid phosphatase were significantly lower (P ＜ 0.05)in CK and CF. Also, the correlations of soil hydrolysable N and available P to soil microbial and enzymatic activities were highly significant (P ＜ 0.01). Thus, to alter the traditional Chinese fir monoculture so as to mimic the natural forest conditions, managing mixed stands of Chinese fir and broadleaf trees or conducting crop rotation of conifers and broadleaf trees as well as minimizing forest disturbances like clear-cutting, slash burning and soil preparing, could be utilized.
文摘UGPase gene related with wood cellulose synthesis was transferred into C. acuminata using the method of Agrobacte- rium-mediated genetic transformation, and an efficient transformation system was developed for C. acuminata on the basis of evaluations of several factors affecting Agrobacterium-mediated DNA transfer rate. The highest transformation rate was achieved when pre-cultttred leaf explants were infected with an Agrobacterium culture corresponding to OD600 （0.5） for 10 min, and cultured on explant regeneration medium for three days. The results of Southern hybridization showed that genomic DNA of the kanamycin-resistant shoots to an UGPase gene probe substantiated the integration of the transgene. Transformation efficiency （6%） was achieved under the optimized transformation procedure, This system should facilitate the introduction of important useful genes into C, acuminata.
