[Objective] The aim was to study the molecular identification and cultivar fingerprints of Prunus persica (L.) Batsch germplasms.[Method] Sixty peach genotypes,representing China common local cultivars and European sa...[Objective] The aim was to study the molecular identification and cultivar fingerprints of Prunus persica (L.) Batsch germplasms.[Method] Sixty peach genotypes,representing China common local cultivars and European samples were screened by microsatellites (simple sequence repeats,SSRs) and Inter-Simple Sequence Repeat (ISSR) markers.[Result] 26 reproducible bands were amplified by Nine SSR primers,and 24 of which were polymorphic; 236 bands were amplified by 30 ISSR primers,and 113 of which were polymorphic.31 genotypes were discriminated with 1-3 distinct polymorphic bands generated from the primers ISSR and SSR.Seven cultivar-specific ISSR fragments and two SSR unique alleles obtained from this study were available to be converted into Sequence Characterized Amplified Region (SCAR) markers.The genetic similarity coefficient (GS) estimated from these molecular data averaged were 0.939 (ranged from 0.856 to 0.983) for ISSR and 0.646 (ranged from 0.240 to 1.000) for SSR,respectively.The combined grouping association indicated that most local Chinese peach cultivars and exotic accessions were clustered together.This could be related to the mode of introduction and maintenance of the peach cultivars involving limited foundation germplasm,exchange of cultivars between plantations,and periodic development of new recombinant cultivars following sexual reproduction.[Conclusion] The results obtained in this work would help to improve the conservation,molecular identification and management of peach germplasm in breeding.展开更多
The experiment was carried out in a private orchard situated at Atrush town, Duhok Govemorate Kurdistan Region-Iraq during 2011 season to study the effect of foliar application of three concentrations of potassium (0...The experiment was carried out in a private orchard situated at Atrush town, Duhok Govemorate Kurdistan Region-Iraq during 2011 season to study the effect of foliar application of three concentrations of potassium (0.0%, 0.5% and 1%) and three concentrations of copper (0.0%, 0.02% and 0.04%) on an eight years old peach cultivar, planted in clay soil, spaced at 4.0 m x 4.0 m. The foliar application was done twice, on April 24, 2011 and May 25, 2011. The results showed that spraying of potassium at both concentration (0.5% and 1%) or copper at high concentration (0.04%) had a positive effect on leaf area, leaf fresh weight, leaf dry weight, total chlorophyll, fruit weight, fruit number and yield per tree as well as fruit diameter, pith thick, pulp weight, seed weight and total soluble solid. The interaction between potassium and copper significantly enhanced all detected traits, since trees receiving 1% and sprayed with 0.04% of copper was characterized by the highest values of all growth and yield characteristics compared to the lowest values at untreated trees (control).展开更多
基金Supported by National Peach Industrial Technology System (nycytx-31-zs-10 )National Science and Technology Support Program (2008BAD98B03-08)+1 种基金National Peach Commonweal Science (Agriculture) Research Projects (3-37)Chengdu Technology Application and Promotion Program (09YTZD986NC-012)
文摘[Objective] The aim was to study the molecular identification and cultivar fingerprints of Prunus persica (L.) Batsch germplasms.[Method] Sixty peach genotypes,representing China common local cultivars and European samples were screened by microsatellites (simple sequence repeats,SSRs) and Inter-Simple Sequence Repeat (ISSR) markers.[Result] 26 reproducible bands were amplified by Nine SSR primers,and 24 of which were polymorphic; 236 bands were amplified by 30 ISSR primers,and 113 of which were polymorphic.31 genotypes were discriminated with 1-3 distinct polymorphic bands generated from the primers ISSR and SSR.Seven cultivar-specific ISSR fragments and two SSR unique alleles obtained from this study were available to be converted into Sequence Characterized Amplified Region (SCAR) markers.The genetic similarity coefficient (GS) estimated from these molecular data averaged were 0.939 (ranged from 0.856 to 0.983) for ISSR and 0.646 (ranged from 0.240 to 1.000) for SSR,respectively.The combined grouping association indicated that most local Chinese peach cultivars and exotic accessions were clustered together.This could be related to the mode of introduction and maintenance of the peach cultivars involving limited foundation germplasm,exchange of cultivars between plantations,and periodic development of new recombinant cultivars following sexual reproduction.[Conclusion] The results obtained in this work would help to improve the conservation,molecular identification and management of peach germplasm in breeding.
文摘The experiment was carried out in a private orchard situated at Atrush town, Duhok Govemorate Kurdistan Region-Iraq during 2011 season to study the effect of foliar application of three concentrations of potassium (0.0%, 0.5% and 1%) and three concentrations of copper (0.0%, 0.02% and 0.04%) on an eight years old peach cultivar, planted in clay soil, spaced at 4.0 m x 4.0 m. The foliar application was done twice, on April 24, 2011 and May 25, 2011. The results showed that spraying of potassium at both concentration (0.5% and 1%) or copper at high concentration (0.04%) had a positive effect on leaf area, leaf fresh weight, leaf dry weight, total chlorophyll, fruit weight, fruit number and yield per tree as well as fruit diameter, pith thick, pulp weight, seed weight and total soluble solid. The interaction between potassium and copper significantly enhanced all detected traits, since trees receiving 1% and sprayed with 0.04% of copper was characterized by the highest values of all growth and yield characteristics compared to the lowest values at untreated trees (control).