[ Objective] The aim of this study was to investigate effects of various inducers on the expression of cytochrome P450 CYP305 B1 V1 Gene in different tissues of wild mulberry silkworm. [ Method] Referring to the mRNA ...[ Objective] The aim of this study was to investigate effects of various inducers on the expression of cytochrome P450 CYP305 B1 V1 Gene in different tissues of wild mulberry silkworm. [ Method] Referring to the mRNA sequence of CYP305 B1 V1 Gene published in GenBank for wild mulberry silkworm, one pair of primers was designed, and the expression of cytochrome P450 CYP305 B1 V1 Gene in different tissues of wild mulberry silkworm treated by NaF, rutin, cypermethrin and ecdysone was also analyzed by the semi - quantitative RT - PCR. Furthermore, homology comparison and phylogenetic analysis for amino acid sequences of this gene were studied. [ Result] Rutin, cypermethrin and NaF had effects on the expression of P450 CYP305 B1 V1 Gene in different tissues of wild mulberry silkworm, while ecdysone had no significant effect. Homology comparison for amino acids indicated that the amino acid sequence of this gene was the most similar to that of CYP305 B1 gene in Bombyx mori with 100% amino acid identity, and highly similar to those of Tribolium casmneum CYP305A1, Apis mellifera CYP305A1, Drosophi- la melanogaster CYP305A1, Anopheles gambiae CYP305A2and Culex pipiens quinquefasciatus CYP2LI. [ Conclusion] CYP305 B1 V1 Gene of wild mulberry silkworm is likely to mainly take part in the metabolism of exogenous compounds, which is of great significance for revealing the function of cytochrome P450 and the metabolic mechanism of different drugs.展开更多
Since ancient times,mulberry leaf has a prominent intestinal regulatory effect,and the close relationship between intestinal diseases and other diseases has attracted more and more attention.This article reviewed the ...Since ancient times,mulberry leaf has a prominent intestinal regulatory effect,and the close relationship between intestinal diseases and other diseases has attracted more and more attention.This article reviewed the relevant articles on the mulberry leaf in recent years and summarized the research progress of mulberry leaf‘s biological activities and their effects on human intestinal regulation and the corresponding mechanisms.Multiple components in the mulberry leaf can individually or synergistically affect the human intestine,directly and indirectly promote the digestion,absorption,and transport function of the small intestine,adjust the balance of the flora,enhance the barrier function of the intestinal mucosa,improve the immunity of the part,and facilitate the intestine recovery from illness.展开更多
Silkworm silk fiber is an attractive material owing to its remarkable mechanical characteristics,excellent optical properties,and good biocompatibility and biodegradability.However,nano-processing of the silk fiber is...Silkworm silk fiber is an attractive material owing to its remarkable mechanical characteristics,excellent optical properties,and good biocompatibility and biodegradability.However,nano-processing of the silk fiber is still a challenge limiting its applications in nanoengineering and related fields.Herein,we report localized near-field enhancement-assisted ablation with an ultrafast laser to break this bottleneck.Localized processing of silk fiber,including nano-holing,nano-grooving,and cutting could retain the key molecular structure building blocks and the pristine functionality of the silk fiber.An extremely narrow nanohole with a width of^64 nm was successfully achieved.The processed silk fiber can be used to transfer micro/nanoparticles and drugs,showing potential for biomedical engineering.The processing strategy developed in this study can also be extended to other materials,paving a new way for fabricating functional nanostructures with precisely controlled size and morphology.展开更多
文摘[ Objective] The aim of this study was to investigate effects of various inducers on the expression of cytochrome P450 CYP305 B1 V1 Gene in different tissues of wild mulberry silkworm. [ Method] Referring to the mRNA sequence of CYP305 B1 V1 Gene published in GenBank for wild mulberry silkworm, one pair of primers was designed, and the expression of cytochrome P450 CYP305 B1 V1 Gene in different tissues of wild mulberry silkworm treated by NaF, rutin, cypermethrin and ecdysone was also analyzed by the semi - quantitative RT - PCR. Furthermore, homology comparison and phylogenetic analysis for amino acid sequences of this gene were studied. [ Result] Rutin, cypermethrin and NaF had effects on the expression of P450 CYP305 B1 V1 Gene in different tissues of wild mulberry silkworm, while ecdysone had no significant effect. Homology comparison for amino acids indicated that the amino acid sequence of this gene was the most similar to that of CYP305 B1 gene in Bombyx mori with 100% amino acid identity, and highly similar to those of Tribolium casmneum CYP305A1, Apis mellifera CYP305A1, Drosophi- la melanogaster CYP305A1, Anopheles gambiae CYP305A2and Culex pipiens quinquefasciatus CYP2LI. [ Conclusion] CYP305 B1 V1 Gene of wild mulberry silkworm is likely to mainly take part in the metabolism of exogenous compounds, which is of great significance for revealing the function of cytochrome P450 and the metabolic mechanism of different drugs.
基金The Key Generic Technology Research Project of High-Quality Agricultural Development of Hebei Province(Grant No.20327121D)Biomedical Development and Transformation Research Project of Hebei Province(Grant No.2020TXZH05)Science and Technology Research Project of Higher Education Institutions of Hebei Province(Grant No.2019188)。
文摘Since ancient times,mulberry leaf has a prominent intestinal regulatory effect,and the close relationship between intestinal diseases and other diseases has attracted more and more attention.This article reviewed the relevant articles on the mulberry leaf in recent years and summarized the research progress of mulberry leaf‘s biological activities and their effects on human intestinal regulation and the corresponding mechanisms.Multiple components in the mulberry leaf can individually or synergistically affect the human intestine,directly and indirectly promote the digestion,absorption,and transport function of the small intestine,adjust the balance of the flora,enhance the barrier function of the intestinal mucosa,improve the immunity of the part,and facilitate the intestine recovery from illness.
基金the support from the National Key R&D Program of China(2017YFB1104300,2016YFA0200103 and 2018YFB1107200)the National Program for the Support of Top-notch Young Professionalsthe National Natural Science Foundation of China(51775303)。
文摘Silkworm silk fiber is an attractive material owing to its remarkable mechanical characteristics,excellent optical properties,and good biocompatibility and biodegradability.However,nano-processing of the silk fiber is still a challenge limiting its applications in nanoengineering and related fields.Herein,we report localized near-field enhancement-assisted ablation with an ultrafast laser to break this bottleneck.Localized processing of silk fiber,including nano-holing,nano-grooving,and cutting could retain the key molecular structure building blocks and the pristine functionality of the silk fiber.An extremely narrow nanohole with a width of^64 nm was successfully achieved.The processed silk fiber can be used to transfer micro/nanoparticles and drugs,showing potential for biomedical engineering.The processing strategy developed in this study can also be extended to other materials,paving a new way for fabricating functional nanostructures with precisely controlled size and morphology.
