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“桥抗体”对酶免疫分析的影响 被引量:7
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作者 洪孝庄 荣康泰 +1 位作者 韩树森 龚雄麒 《军事医学科学院院刊》 CSCD 北大核心 1990年第3期203-208,共6页
在对 T-2毒素检测的 ELISA 研究中,T2-HS(T2-琥珀酸半酯)与 BSA 或 PLL联接,前者(T2-C_4-BSA)为免疫原,后者(T2-C_4-PLL)为检测抗原。在进行 ELISA 时,将 T2-C_4-PLL 包被于微板,并与兔抗 T-2抗体和标准 T-2样品一起保温。T-2抗体结合... 在对 T-2毒素检测的 ELISA 研究中,T2-HS(T2-琥珀酸半酯)与 BSA 或 PLL联接,前者(T2-C_4-BSA)为免疫原,后者(T2-C_4-PLL)为检测抗原。在进行 ELISA 时,将 T2-C_4-PLL 包被于微板,并与兔抗 T-2抗体和标准 T-2样品一起保温。T-2抗体结合量用酶(HRP)标羊抗兔 IgG 与酶底物反应进行测定。在此情况下,我们不仅观察到抗 T-2抗体及抗 BSA 抗体,而且还有抗“-C_4-”抗体(即“桥抗体”)存在,同时发现“桥抗体”对T-2检测的灵敏度有明显干扰,甚至使 ELISA 不能进行。如以 T2-C_2-TT 代替 T2-C_4-PLL为检测抗原,由于“-C_4-”桥的排除,“桥抗体”的干扰也明显减弱。 展开更多
关键词 T-2毒素 酶免疫分析 桥抗体
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用单克隆抗体桥联酶标法诊断小儿呼吸道病毒感染 被引量:1
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作者 孙康德 吴斌 +2 位作者 王玫琦 周慧君 虞中敏 《临床检验杂志》 CAS CSCD 北大核心 2001年第4期232-233,共2页
关键词 碱性磷酸酶-抗碱性磷酸酶 单克隆抗体联酶标技术 呼吸道病毒感染 儿童
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免疫酶法检测人的ANA
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作者 罗祝泉 《细胞与分子免疫学杂志》 CAS CSCD 1994年第4期24-24,共1页
免疫酶法检测人的ANA罗祝泉(广州军区广州总医院病理科,广州510010)抗核抗体(ANA)传统的方法是用间接免疫荧光法来检测,由于受设备条件限制,使一些单位不能开展.用免疫酶法来检测,亦能获得与间接免疫荧光法相同的... 免疫酶法检测人的ANA罗祝泉(广州军区广州总医院病理科,广州510010)抗核抗体(ANA)传统的方法是用间接免疫荧光法来检测,由于受设备条件限制,使一些单位不能开展.用免疫酶法来检测,亦能获得与间接免疫荧光法相同的效果.现介绍如下.1材料和方法1.... 展开更多
关键词 免疫酶法 ANA 间接免疫荧光法 抗核抗体 抗原片 病人血清 抗体反应 桥抗体 冰冻切片 动物血清
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用单克隆抗体桥联酶标法诊断小儿呼吸道病毒感染
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作者 孙康德 王玫琦 +1 位作者 周慧君 虞中敏 《中华微生物学和免疫学杂志》 CAS CSCD 北大核心 2001年第S1期114-115,共2页
目的 探讨小儿患者呼吸道病毒感染的病原诊断。方法 采用单克隆抗体桥联酶标法。结果 在 40例小儿患者中 ,阳性有 17例 ,占 42 .5 %。其中呼吸道合胞病毒 (RespiratorySyncytialVirus,RSV)占 2 0 % ,流感病毒 (InfluenzaVirus,Flu)占... 目的 探讨小儿患者呼吸道病毒感染的病原诊断。方法 采用单克隆抗体桥联酶标法。结果 在 40例小儿患者中 ,阳性有 17例 ,占 42 .5 %。其中呼吸道合胞病毒 (RespiratorySyncytialVirus,RSV)占 2 0 % ,流感病毒 (InfluenzaVirus,Flu)占 12 .5 % ,副流感病毒 (ParainfluenzaVirus ,PIV)占7.5 % ,腺病毒 (Adenovirus,Adv)占 2 .5 %。结论 用单克隆抗体桥联酶标法诊断小儿呼吸道病毒是一种灵敏度高。 展开更多
关键词 碱性磷酸酶-抗碱性磷酸酶 单克隆抗体联酶标技术 呼吸道病毒
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Scaled down model design of shaking table test of Taizhou Bridge 被引量:1
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作者 Yan Jukao Han Lei Zhao Yi Peng Tianbo 《Engineering Sciences》 EI 2012年第3期33-37,共5页
Taizhou Bridge is the first kilometer level three-pylon two-span suspension bridge in the world and the structural complexity has significant effects on the seismic performance of the bridge. Shaking table test of Tai... Taizhou Bridge is the first kilometer level three-pylon two-span suspension bridge in the world and the structural complexity has significant effects on the seismic performance of the bridge. Shaking table test of Taizhou Bridge is arranged to investigate the effects of non-uniform ground motion input, collision between main and side spans and optimal seismic structural system. It's very important and difficult to design and manufacture the scaled down model of Taizhou Bridge used during the shaking table test. The key point is that the girder and pylons are very hard to be manufactured if the similarity ratio is strictly followed. Based on the finite element method (FEM) analysis, a simplified scaled down model is designed and the bending stiffness of the girder and pylon are strictly simulated, and the torsion stiffness and axial stiffness are not strictly simulated. The inner forces and displacements of critical sections, points of simplified model and theoretical model are compared by FEM analysis, and it's found out that the difference between the seismic responses is relatively small. So, the simplified model can be used to conduct the shaking table test by the FEM verification. 展开更多
关键词 three-pylon two-span suspension bridge shaking table test stiffness equivalence simplified scaled down model FEM verification
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Design of steel box girder for Taizhou Yangtze River Highway Bridge
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作者 Ding Lei Shan Hongwei Zhou Qing Zheng Benhui 《Engineering Sciences》 EI 2011年第2期57-63,共7页
Taizhou Yangtze River Highway Bridge is the first three-pylon two-span suspension bridge in China. The main girder adopts flat steamline steel closed box girder which has well wind-resistant capability and is technica... Taizhou Yangtze River Highway Bridge is the first three-pylon two-span suspension bridge in China. The main girder adopts flat steamline steel closed box girder which has well wind-resistant capability and is technically mature besides beautiful appearance. Straight web plates of the steel box girder in longitudinal direction are proposed in order to ensure the integrity of the steel box girder, and to keep the stress of the steel box girder continuous in the middle pylon, as well as to reduce the gradient of the middle pylon columns. The cross section of the box girder has one box with three cells. Solid-web diaphragm plate with good integrity and high torsional stiffness is adopted. The lifting lugs are utilized in the anchors of suspender cable. In this paper, selection of the cross section of the steel box girder, the general structure design, local structure design and main structure calculation results of Taizhou Yangtze River Bridge are introduced emphatically. 展开更多
关键词 Taizhou Yangtze River Highway Bridge three-pylon suspension bridge steel box girder DESIGN
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