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不同激素对‘美人’梅叶片离体培养的影响及其细胞学观察 被引量:2
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作者 张秦英 陈俊愉 申作连 《北京林业大学学报》 CAS CSCD 北大核心 2004年第S1期42-44,169,共4页
该试验以‘美人’梅离体培养的无菌苗叶片为材料 ,研究了不同种类激素配比对叶片脱分化及再分化的影响 ,并对不同条件下产生的愈伤组织进行了细胞学观察。结果表明 :BA对‘美人’梅叶片的愈伤组织诱导起到抑制作用 ;NAA可使叶片直接生根... 该试验以‘美人’梅离体培养的无菌苗叶片为材料 ,研究了不同种类激素配比对叶片脱分化及再分化的影响 ,并对不同条件下产生的愈伤组织进行了细胞学观察。结果表明 :BA对‘美人’梅叶片的愈伤组织诱导起到抑制作用 ;NAA可使叶片直接生根 ;2 ,4 D的加入可以诱发叶片形成球形愈伤。但只有配合适当浓度的BA ,才能形成胚状体。诱导胚状体合适的培养基为 :WPM +BA 1mg L +NAA 0 1mg L +2 ,4 D 1mg L +L 谷氨酰胺 10 0mg L +水解乳蛋白 10 0 0mg L。 展开更多
关键词 梅叶片 激素 不定根 体细胞胚发生 细胞观察
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Effects of Remaining Leaf Combining with IBA on Rooting,Physiological and Biochemical Indicators of Leaves from Bougainvillea spectabilis Cuttings 被引量:1
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作者 赖瑞云 钟赞华 +3 位作者 张雪芹 林丽仙 苏明华 谢志南 《Agricultural Science & Technology》 CAS 2010年第7期120-123,共4页
[Objective] The research aimed to discuss the effects on rooting,physiological and biochemical indicators of Bougainvillea spectabilis cuttings by remaining leaf combining with soaking cuttings in IBA.[Method] Effects... [Objective] The research aimed to discuss the effects on rooting,physiological and biochemical indicators of Bougainvillea spectabilis cuttings by remaining leaf combining with soaking cuttings in IBA.[Method] Effects of remaining leaf combining with IBA on rooting,physiological and biochemical indicators of B.spectabilis cuttings were studied under the regulation and control of intelligent seed propagation system,and then set four treatments:remaining leaf combining with soaking in clean water,remaining leaf combining with soaking in IBA,defoliation combining soaking in clean water,and defoliation combining with soaking in IBA.[Result] The soluble sugar content was high,but indoleacetic acid oxidase activity was lower in the initial stage of adventitious root differentiation in treatment of remaining leaf.While the rooting number,root length,the root mass growth and survival rate of cutting were obviously higher than that in treatment of defoliation.Compared to the treatment soaking in clean water,the treatment soaking in 500×10^-6 IBA increased the soluble sugar content in leaves of cuttings and enhanced rooting of cuttings.[Conclusion] The survival rate and rooting of cuttings improved obviously in the treatment of remaining leaf combining with soaking in 500×10^-6 IBA before cutting under the regulation and control of intelligent seed propagation system. 展开更多
关键词 LEAF IBA Bougainvillea spectabilis cutting ROOTING Physiological and biochemical indicators
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