The salt-gradient operation mode used in ion-exchange simulated moving bed chromatography (SMBC) can improve the efficiency of protein separations. A detailed model that takes into account any kind of adsorption/ion-e...The salt-gradient operation mode used in ion-exchange simulated moving bed chromatography (SMBC) can improve the efficiency of protein separations. A detailed model that takes into account any kind of adsorption/ion-exchange equilibrium, salt gradient, size exclusion, mass transfer resistance, and port periodic switching mechanism, was developed to simulate the complex dynamics. The model predictions were verified by the experimental data on upward and downward gradients for protein separations reported in the literature. All design and operating parameters (number, configuration, length and diameter of columns, particle size, switching period, flow rates of feed, raffinate, desorbent and extract, protein concentrations in feed, different salt concentrations in desorbent and feed) can be chosen correctly by numerical simulation. This model can facilitate the design, operation, optimization, control and scale-up of salt-gradient ion-exchange SMBC for protein separations.展开更多
To explore the spatial heterogeneity of plankton communities in a deep artificial lake (Songhua Lake, China), samples were collected at seven sites. Samples were investigated by denaturing gradient gel electrophores...To explore the spatial heterogeneity of plankton communities in a deep artificial lake (Songhua Lake, China), samples were collected at seven sites. Samples were investigated by denaturing gradient gel electrophoresis (DGGE) analysis of the PCR-amplified 16S and 18S rRNA genes and specific bands were sequenced. Cluster analysis of the DGGE profiles revealed that all of the samples grouped into two distinct clusters, in accordance with sampling site; while in each cluster, the divergence of sub-clusters correlated with sampling depth. Sequence analysis of selected dominant DGGE bands revealed that most sequenced phylotypes (84%) exhibited 〉97% similarity to the closest sequences in GenBank, and were affiliated with ten common freshwater plankton phyla (Proteobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, Bacillariophyta, Pyrrophyta, Cryptophyta, Ciliophora, Stramenopiles, and Rotifera). Several of these groups are also found worldwide, indicating the cosmopolitan distribution of the phylotypes. The relationships between DGGE patterns and environmental factors were analyzed by redundancy analysis (RDA). The results suggested that, total nitrogen, nitrate, nitrite, temperature were strongly correlated with the variation ammonia, and CODMn concentrations, and water in plankton composition.展开更多
This paper focuses on the indicators of soil and litter health, disturbance, and landscape heterogeneity as a tool for prediction of ecosystem sustainability in the northern forests of Iran. The study area was divided...This paper focuses on the indicators of soil and litter health, disturbance, and landscape heterogeneity as a tool for prediction of ecosystem sustainability in the northern forests of Iran. The study area was divided into spatial homogenous sites using slope, aspect, and soil humidity classes. Then a range of sites along the disturbance gradient was selected for sampling. Chemical and physical indicators of soil and litter health were measured at random points within these sites. Structural equation modeling(SEM) was applied to link six constructs of landscape heterogeneity, three constructs of disturbance(harvest, livestock, and human accessibility), and soil and litter health. The results showed that with decreasing accessibility, the total N and organic matter content of soil increased and effective bulk density decreased. Harvesting activities increased soil organic matter. Therefore, it is concluded that disturbances through harvesting and accessibility inversely affect the soil health. Unexpectedly, it was found that the litter total C and C:N ratio improved with an increase in the harvest and accessibility disturbances, whereas litter bulk density decreased. Investigation of tree composition revealed that in the climax communities, which are normally affected more by harvesting activities, some species like Fagus orientalis Lipsky with low decomposition rate are dominant. The research results showed that changes in disturbance intensity are reflected in litter and soil indicators, whereas the SEM indicated that landscape heterogeneity has a moderator effect on the disturbance to both litter and soil paths.展开更多
The first modem survey of the Dead Sea was performed by the Geological Survey of Israel in 1959-1960, and the report published remains the baseline study for our understanding of the physical and chemical properties o...The first modem survey of the Dead Sea was performed by the Geological Survey of Israel in 1959-1960, and the report published remains the baseline study for our understanding of the physical and chemical properties of the lake. At the time the Dead Sea was a meromictic lake with a strong salinity gradient separating the deep waters (〉40 m depth) from the less saline surface waters. A few results of analyses of deeper water samples collected in the 1930s were reported, but overall we have very little information about the structure of the lake's water column before the 1959-1960 survey. However, it is little known that data on the physical and the chemical structure of the Dead Sea water column were obtained already in the middle of the 19~ century, and the information collected then is highly relevant for the reconstruction of the limnological properties of the lake in earlier times. The expedition of Lieutenant William Lynch (U.S. Navy) in 1848 reported the presence of a temperature minimum at a depth of-18 m, and also retrieved a water sample collected close to the bottom for chemical analysis. In 1864, the French Dead Sea exploration by the Duc de Luynes and his crew yielded detailed density and salinity profiles for a number of sampling stations. The results of these pioneering studies are discussed here, as well as the sampling equipment and measuring instruments used by the 1848 and the 1864 expeditions.展开更多
The effects of long-term (19 years) different land use and fertilization on activity and composition of ammonia-oxidizing bacteria (AOB) in an aquic brown soil were investigated in a field experiment in Liaoning Provi...The effects of long-term (19 years) different land use and fertilization on activity and composition of ammonia-oxidizing bacteria (AOB) in an aquic brown soil were investigated in a field experiment in Liaoning Province,China.The 19-year experiment conducted from 1990 to 2008 involved seven treatments designed: cropping rotation of soybean-corn-corn with no fertilizer (control,CK),recycled manure (RM),fertilizer nitrogen (N),phosphorous (P) and potassium (K) (NPK),NPK+RM,and no-crop bare land,mowed fallow,and non-mowed fallow.The results showed that the potential nitrification rates of the RM,NPK+RM,mowed fallow,and non-mowed fallow treatments were significantly higher (P < 0.05) than those of the CK and NPK treatments,indicating that the long-term applications of recycled manure and return of plant residues both significantly increased the activity of AOB.Although the application of NPK did not enhance soil potential nitrification because of decreased pH,available K had an important effect on potential nitrification.Denaturing gradient gel electrophoresis (DGGE) fingerprint profiles showed that no-crop treatments had an increase in the diversity of the AOB community compared to the CK,RM,and NPK treatments,implying that agricultural practices,especially tillage,had an adverse effect on the soil AOB community.The NPK+RM treatment had the most diverse DGGE patterns possibly because of the increased available P in this treatment.A phylogenetic analysis showed that most of the DGGE bands derived belonged to Nitrosospira cluster 3,not Nitrosospira cluster 2.These demonstrated that different land use and fertilization significantly influenced the activity and composition of the AOB community by altering the soil properties,mainly including pH,total C,available K,and available P.展开更多
In this paper,the behavior of a salt-gradient solar pond with the square cross-section has been studied experimentally and numerically.A small-scale solar pond were designed and built to provide quantitative data.A tw...In this paper,the behavior of a salt-gradient solar pond with the square cross-section has been studied experimentally and numerically.A small-scale solar pond were designed and built to provide quantitative data.A two-dimensional,transient heat and mass transfer model has been solved numerically by using finite-control-volume method.In this study,all the thermo-physical properties are variable as the function of temperature and salt concentration.Numerical results as obtained for the experimental pond have been satisfactorily compared and validated against measured data.Furthermore,the wall shading effect has been elaborated to improve the agreement between two sets of results.The temperature of the storage zone is predicted well by the model.It also can be observed that the initial concentration profile is preserved with time.The stability of the pond in time has been investigated in order to distinguish the critical zones.Finally,the application of an energy analysis gives an efficiency of about 12%for the pond.展开更多
H61der and gradient estimates for the correctors in the homogenization are presented based on the translation invariance and Li-Vogelius's gradient estimate. If the coefficients are piecewise smooth and the homogeniz...H61der and gradient estimates for the correctors in the homogenization are presented based on the translation invariance and Li-Vogelius's gradient estimate. If the coefficients are piecewise smooth and the homogenized solution is smooth enough, the interior error of the first-order expansion is O(e) in the HSlder norm; it is O(e) in W1,∞ based on the Avellaneda-Lin's gradient estimate when the coefficients are Lipschitz continuous. These estimates can be partly extended to the nonlinear parabolic equations.展开更多
This study is about an analytical attempt that explores the two-dimensional concentration distribution of a solute in an open channel flow.The solute undergoes reversible sorption at the channel bed.The method of mult...This study is about an analytical attempt that explores the two-dimensional concentration distribution of a solute in an open channel flow.The solute undergoes reversible sorption at the channel bed.The method of multiple scales is used to find the two-dimensional concentration distribution,which is important for modem day application in industry,environmental risk assessment,etc.Study deduces an analytic expression of two-dimensional concentration distribution for an open channel flow with sorptive channel bed.Effects of retention parameter,Darnkohler number on the solute dispersion are also discussed in this paper.Results reveal that slow or strong kinetics(small value of Darnkohler number)increases solute dispersion.It is also observed.that for slow phase exchange kinetics between bulk flow and small retentive channel bed,solute concentration distribution will uniform faster than their inert counterpart.展开更多
文摘The salt-gradient operation mode used in ion-exchange simulated moving bed chromatography (SMBC) can improve the efficiency of protein separations. A detailed model that takes into account any kind of adsorption/ion-exchange equilibrium, salt gradient, size exclusion, mass transfer resistance, and port periodic switching mechanism, was developed to simulate the complex dynamics. The model predictions were verified by the experimental data on upward and downward gradients for protein separations reported in the literature. All design and operating parameters (number, configuration, length and diameter of columns, particle size, switching period, flow rates of feed, raffinate, desorbent and extract, protein concentrations in feed, different salt concentrations in desorbent and feed) can be chosen correctly by numerical simulation. This model can facilitate the design, operation, optimization, control and scale-up of salt-gradient ion-exchange SMBC for protein separations.
基金Supported by the National Natural Science Foundation of China(No.51178090)the National Key Technology R&D Program of China(No.2012BAJ21B02-02)the National Water Pollution Control and Management Technology Major Projects(No.2009ZX07106-001)
文摘To explore the spatial heterogeneity of plankton communities in a deep artificial lake (Songhua Lake, China), samples were collected at seven sites. Samples were investigated by denaturing gradient gel electrophoresis (DGGE) analysis of the PCR-amplified 16S and 18S rRNA genes and specific bands were sequenced. Cluster analysis of the DGGE profiles revealed that all of the samples grouped into two distinct clusters, in accordance with sampling site; while in each cluster, the divergence of sub-clusters correlated with sampling depth. Sequence analysis of selected dominant DGGE bands revealed that most sequenced phylotypes (84%) exhibited 〉97% similarity to the closest sequences in GenBank, and were affiliated with ten common freshwater plankton phyla (Proteobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, Bacillariophyta, Pyrrophyta, Cryptophyta, Ciliophora, Stramenopiles, and Rotifera). Several of these groups are also found worldwide, indicating the cosmopolitan distribution of the phylotypes. The relationships between DGGE patterns and environmental factors were analyzed by redundancy analysis (RDA). The results suggested that, total nitrogen, nitrate, nitrite, temperature were strongly correlated with the variation ammonia, and CODMn concentrations, and water in plankton composition.
文摘This paper focuses on the indicators of soil and litter health, disturbance, and landscape heterogeneity as a tool for prediction of ecosystem sustainability in the northern forests of Iran. The study area was divided into spatial homogenous sites using slope, aspect, and soil humidity classes. Then a range of sites along the disturbance gradient was selected for sampling. Chemical and physical indicators of soil and litter health were measured at random points within these sites. Structural equation modeling(SEM) was applied to link six constructs of landscape heterogeneity, three constructs of disturbance(harvest, livestock, and human accessibility), and soil and litter health. The results showed that with decreasing accessibility, the total N and organic matter content of soil increased and effective bulk density decreased. Harvesting activities increased soil organic matter. Therefore, it is concluded that disturbances through harvesting and accessibility inversely affect the soil health. Unexpectedly, it was found that the litter total C and C:N ratio improved with an increase in the harvest and accessibility disturbances, whereas litter bulk density decreased. Investigation of tree composition revealed that in the climax communities, which are normally affected more by harvesting activities, some species like Fagus orientalis Lipsky with low decomposition rate are dominant. The research results showed that changes in disturbance intensity are reflected in litter and soil indicators, whereas the SEM indicated that landscape heterogeneity has a moderator effect on the disturbance to both litter and soil paths.
文摘The first modem survey of the Dead Sea was performed by the Geological Survey of Israel in 1959-1960, and the report published remains the baseline study for our understanding of the physical and chemical properties of the lake. At the time the Dead Sea was a meromictic lake with a strong salinity gradient separating the deep waters (〉40 m depth) from the less saline surface waters. A few results of analyses of deeper water samples collected in the 1930s were reported, but overall we have very little information about the structure of the lake's water column before the 1959-1960 survey. However, it is little known that data on the physical and the chemical structure of the Dead Sea water column were obtained already in the middle of the 19~ century, and the information collected then is highly relevant for the reconstruction of the limnological properties of the lake in earlier times. The expedition of Lieutenant William Lynch (U.S. Navy) in 1848 reported the presence of a temperature minimum at a depth of-18 m, and also retrieved a water sample collected close to the bottom for chemical analysis. In 1864, the French Dead Sea exploration by the Duc de Luynes and his crew yielded detailed density and salinity profiles for a number of sampling stations. The results of these pioneering studies are discussed here, as well as the sampling equipment and measuring instruments used by the 1848 and the 1864 expeditions.
基金Supported by the Innovation Program of the Chinese Academy of Sciences (Nos. KZCX2-YW-407 and KZCX2-YW-405)the National Key Technology R&D Program of China (No. 2006BAD05B05)the National Natural Science Foundationof China (No. 40701067)
文摘The effects of long-term (19 years) different land use and fertilization on activity and composition of ammonia-oxidizing bacteria (AOB) in an aquic brown soil were investigated in a field experiment in Liaoning Province,China.The 19-year experiment conducted from 1990 to 2008 involved seven treatments designed: cropping rotation of soybean-corn-corn with no fertilizer (control,CK),recycled manure (RM),fertilizer nitrogen (N),phosphorous (P) and potassium (K) (NPK),NPK+RM,and no-crop bare land,mowed fallow,and non-mowed fallow.The results showed that the potential nitrification rates of the RM,NPK+RM,mowed fallow,and non-mowed fallow treatments were significantly higher (P < 0.05) than those of the CK and NPK treatments,indicating that the long-term applications of recycled manure and return of plant residues both significantly increased the activity of AOB.Although the application of NPK did not enhance soil potential nitrification because of decreased pH,available K had an important effect on potential nitrification.Denaturing gradient gel electrophoresis (DGGE) fingerprint profiles showed that no-crop treatments had an increase in the diversity of the AOB community compared to the CK,RM,and NPK treatments,implying that agricultural practices,especially tillage,had an adverse effect on the soil AOB community.The NPK+RM treatment had the most diverse DGGE patterns possibly because of the increased available P in this treatment.A phylogenetic analysis showed that most of the DGGE bands derived belonged to Nitrosospira cluster 3,not Nitrosospira cluster 2.These demonstrated that different land use and fertilization significantly influenced the activity and composition of the AOB community by altering the soil properties,mainly including pH,total C,available K,and available P.
文摘In this paper,the behavior of a salt-gradient solar pond with the square cross-section has been studied experimentally and numerically.A small-scale solar pond were designed and built to provide quantitative data.A two-dimensional,transient heat and mass transfer model has been solved numerically by using finite-control-volume method.In this study,all the thermo-physical properties are variable as the function of temperature and salt concentration.Numerical results as obtained for the experimental pond have been satisfactorily compared and validated against measured data.Furthermore,the wall shading effect has been elaborated to improve the agreement between two sets of results.The temperature of the storage zone is predicted well by the model.It also can be observed that the initial concentration profile is preserved with time.The stability of the pond in time has been investigated in order to distinguish the critical zones.Finally,the application of an energy analysis gives an efficiency of about 12%for the pond.
基金supported by National Natural Science Foundation of China (Grant No.90916027)Special Funds for National Basic Research Program of China (973 Program) (Grant No. 2010CB832702)the State Key Laboratory of Scientific and Engineering Computing
文摘H61der and gradient estimates for the correctors in the homogenization are presented based on the translation invariance and Li-Vogelius's gradient estimate. If the coefficients are piecewise smooth and the homogenized solution is smooth enough, the interior error of the first-order expansion is O(e) in the HSlder norm; it is O(e) in W1,∞ based on the Avellaneda-Lin's gradient estimate when the coefficients are Lipschitz continuous. These estimates can be partly extended to the nonlinear parabolic equations.
文摘This study is about an analytical attempt that explores the two-dimensional concentration distribution of a solute in an open channel flow.The solute undergoes reversible sorption at the channel bed.The method of multiple scales is used to find the two-dimensional concentration distribution,which is important for modem day application in industry,environmental risk assessment,etc.Study deduces an analytic expression of two-dimensional concentration distribution for an open channel flow with sorptive channel bed.Effects of retention parameter,Darnkohler number on the solute dispersion are also discussed in this paper.Results reveal that slow or strong kinetics(small value of Darnkohler number)increases solute dispersion.It is also observed.that for slow phase exchange kinetics between bulk flow and small retentive channel bed,solute concentration distribution will uniform faster than their inert counterpart.