[Objective] This study aimed to establish a method for quantitative detection of mRNA transcriptional level of SS2 adhesive related-factors of Streptococcus suis serotype 2 （SS2） by fluorescent quantitative PCR. []V...[Objective] This study aimed to establish a method for quantitative detection of mRNA transcriptional level of SS2 adhesive related-factors of Streptococcus suis serotype 2 （SS2） by fluorescent quantitative PCR. []Vlethod] The gene fragments en- coding SS2 adhesive related-factors MRP, FBPS and CPS2J and a housekeeping gene aroA were amplified by reverse transcription PCR from the total RNA of SS2, cloned, and sequenced. The recombinant plasmids containing the target genes were constructed, and used as templates in Real-time PCR. [Result] Dynamic curves, stan- dard curves and melting curves of the adhesive related-factors and aroA were ob- tained by the optimized Real-time PCR system. The standard curves showed a good linear relationship between template copy number and circulation number, and the correlation coefficients （FF） of the standard curves were over 0.995. Also, these as- says were highly specific a^d there was single specific melting peak for every gene. Moreover, the assays were highly sensitive and had a detection limit of 1.0×102 copies in 1 μl of initial templates. Finally, it was highly repeatable and had a coeffi- cient of variation less than 2% for intra-assay. [Conclusion] This study will provide a way to reveal the adhesion mechanism of SS2 to different host cells at molecular level.展开更多
The height changes of tide gauges directly influence sea level observation. For research of sea level variation in large region or globe, the land vertical displacement must be considered. Two sessions of GPS and abso...The height changes of tide gauges directly influence sea level observation. For research of sea level variation in large region or globe, the land vertical displacement must be considered. Two sessions of GPS and absolute gravity observations in 2001 and 2003 are used to determine the horizontal and vertical motion of China coast in ITRF2000 and Eurasia frame. The difference between results of continuous observation and periodic observation is discussed.展开更多
The spread of social media has increased contacts of members of communities on the lntemet. Members of these communities often use account names instead of real names. When they meet in the real world, they will find ...The spread of social media has increased contacts of members of communities on the lntemet. Members of these communities often use account names instead of real names. When they meet in the real world, they will find it useful to have a tool that enables them to associate the faces in fiont of them with the account names they know. This paper proposes a method that enables a person to identify the account name of the person （＂target＂） in front of him/her using a smartphone. The attendees to a meeting exchange their identifiers （i.e., the account name） and GPS information using smartphones. When the user points his/her smartphone towards a target, the target＇s identifier is displayed near the target＇s head on the camera screen using AR （augmented reality）. The position where the identifier is displayed is calculated from the differences in longitude and latitude between the user and the target and the azimuth direction of the target from the user. The target is identified based on this information, the face detection coordinates, and the distance between the two. The proposed method has been implemented using Android terminals, and identification accuracy has been examined through experiments.展开更多
基金Supported by National Natural Science Foundation of China(31072155)Natural Science Foundation of Jiangsu Province(BK2010068)+1 种基金Fund for Independent Innovation of Agricultural Science in Jiangsu Province[CX(11)2060]Special Fund for Agroscientific Research in the Public Interest(201303041)~~
文摘[Objective] This study aimed to establish a method for quantitative detection of mRNA transcriptional level of SS2 adhesive related-factors of Streptococcus suis serotype 2 （SS2） by fluorescent quantitative PCR. []Vlethod] The gene fragments en- coding SS2 adhesive related-factors MRP, FBPS and CPS2J and a housekeeping gene aroA were amplified by reverse transcription PCR from the total RNA of SS2, cloned, and sequenced. The recombinant plasmids containing the target genes were constructed, and used as templates in Real-time PCR. [Result] Dynamic curves, stan- dard curves and melting curves of the adhesive related-factors and aroA were ob- tained by the optimized Real-time PCR system. The standard curves showed a good linear relationship between template copy number and circulation number, and the correlation coefficients （FF） of the standard curves were over 0.995. Also, these as- says were highly specific a^d there was single specific melting peak for every gene. Moreover, the assays were highly sensitive and had a detection limit of 1.0×102 copies in 1 μl of initial templates. Finally, it was highly repeatable and had a coeffi- cient of variation less than 2% for intra-assay. [Conclusion] This study will provide a way to reveal the adhesion mechanism of SS2 to different host cells at molecular level.
文摘The height changes of tide gauges directly influence sea level observation. For research of sea level variation in large region or globe, the land vertical displacement must be considered. Two sessions of GPS and absolute gravity observations in 2001 and 2003 are used to determine the horizontal and vertical motion of China coast in ITRF2000 and Eurasia frame. The difference between results of continuous observation and periodic observation is discussed.
文摘The spread of social media has increased contacts of members of communities on the lntemet. Members of these communities often use account names instead of real names. When they meet in the real world, they will find it useful to have a tool that enables them to associate the faces in fiont of them with the account names they know. This paper proposes a method that enables a person to identify the account name of the person （＂target＂） in front of him/her using a smartphone. The attendees to a meeting exchange their identifiers （i.e., the account name） and GPS information using smartphones. When the user points his/her smartphone towards a target, the target＇s identifier is displayed near the target＇s head on the camera screen using AR （augmented reality）. The position where the identifier is displayed is calculated from the differences in longitude and latitude between the user and the target and the azimuth direction of the target from the user. The target is identified based on this information, the face detection coordinates, and the distance between the two. The proposed method has been implemented using Android terminals, and identification accuracy has been examined through experiments.
