为了克服牛奶质量检测方法存在的检测设备昂贵、分析周期长、难以在日常生活中应用等问题,分析并验证射频识别(Radio Frequency Identification,RFID)技术进行牛奶质量检测的方法,设计了基于RFID标签天线的牛奶质量传感器,通过仿真进行...为了克服牛奶质量检测方法存在的检测设备昂贵、分析周期长、难以在日常生活中应用等问题,分析并验证射频识别(Radio Frequency Identification,RFID)技术进行牛奶质量检测的方法,设计了基于RFID标签天线的牛奶质量传感器,通过仿真进行性能优化以及实验验证,分析不同介电参数的牛奶对RFID谐振频率的影响,进而根据频率的偏移量判断牛奶是否变质。设计的天线最大近场增益达到74.877 0dB,耦合时的辐射效率达到了95.1%.在用于牛奶检测方面,优化的天线比普通商用天线具有更高的灵敏度。展开更多
This paper presents a process which allows the determination of milk adulteration by water. Considering the limitations of the reference method （cryoscopy）, the proposed methodology matches electrical conductivity a...This paper presents a process which allows the determination of milk adulteration by water. Considering the limitations of the reference method （cryoscopy）, the proposed methodology matches electrical conductivity and cryoscopy index measurements. Based on statistical procedures, a linear descriptive model is achieved to quantify adulteration of milk with water. It is also shown that electrical conductivity measurements allow the detection of adulteration with water and sodium chloride.展开更多
To compare mid-infrared(MIR)and near-infrared(NIR)spectroscopies for the determination of the fat and protein contents in milk,the same sample sets with varying concentrations of fat and protein were measured in the M...To compare mid-infrared(MIR)and near-infrared(NIR)spectroscopies for the determination of the fat and protein contents in milk,the same sample sets with varying concentrations of fat and protein were measured in the MIR range of 3 200-700 cm-1 and NIR range of 9 000-4 000 cm-1.The spectral features in the two regions were analyzed.The MIR spectra of milk were characteristic due to the MIR inherent molecular specificity,whereas the NIR spectra were relatively characterless due to the NIR low selectivity.Partial least squares(PLS)regression models for fat and protein were developed by using both MIR and NIR spectra.MIR data with no pretreatment gave better results than NIR data.The square correlation coefficient(R2)and the root mean square error of prediction(RMSEP)were 0.98 and 0.10 g/dL for fat and 0.97 and 0.11 g/dL for protein.With NIR techniques,satisfactory results were not obtained with raw data.However,NIR data after pretreatment gave similarly good results to the ones using MIR method.This paper indicates that either of the MIR and NIR spectral methods is reliable for the determination of the fat and protein contents.展开更多
The purpose of this paper is double： first to measure the natural radioactivity in any food by detecting the omnipresent radiation of ^40K, and then to use this date to obtain the concentration of the important miner...The purpose of this paper is double： first to measure the natural radioactivity in any food by detecting the omnipresent radiation of ^40K, and then to use this date to obtain the concentration of the important mineral nutrient K, by dividing the specific activity of the sample （Bq/g） by the specific activity of elementary K （31.19 Bq/g）. The method implies the γ rays detection emitted by natural ^40K-^40Ar present in foodstuff, by using a 3 × 3＂ low background NaI （TI） scintillation detector coupled to a PC charged with the Maestro Program. When this detection system is calibrated in order to determine its efficiency for 1461 KeV γrays emitted during ^40K-^40Ar decaying, it is possible to measure easily and with enough precision the specific activity due to natural ^40K present in any foodstuff. Once this figure is divided by the constant value of ^40K specific activity of elementary K, the concentration of this element in the foodstuff sample is established. This paper describes the procedure and shows the results obtained with four basic diet products： milk （powdered, light and cream）, eggs （yellow and white）, wheat （whole and refined flour） and corn （whole and cornstarch flour）. The possible application of this study seems to be to establish the necessary threshold to evaluate the importance of any possible radioactive contamination, as well as to measure the concentration of the mineral nutrient K in foodstuff, by means of an easy, practicable method. Also, it might be useful as an experiment for teaching purposes.展开更多
The main purpose of this study was to detect an association of cytoplasmic signal transducers and activators of transcription-1 （STAT1） with milk production traits in 472 Holstein and 283 Jersey cattle breeds of Tur...The main purpose of this study was to detect an association of cytoplasmic signal transducers and activators of transcription-1 （STAT1） with milk production traits in 472 Holstein and 283 Jersey cattle breeds of Turkey. This gene, located on chromosome 2, was chosen due to its role on development of mammary gland. A polymorphism of selected 314 bp allele fragment was detected by the restriction fragment length polymorphism analysis of polymerase chain reaction-amplified fragments （PCR-RFLP） method and also confirmed by DNA sequencing. The association tests were conducted between STAT1 genotypes and some economically important dairy traits. The genotypes for C/T as a single nucleotide polymorphism （SNP） were identified at interval 60 cM to 63 cM. The effects of STAT1 gene on milk production traits were not significant in Holstein cows, although animals with CT genotypes showed fairly close to significant value for the corrected 305 d milk yield. However, Jersey cows with/7＂ genotype were 2.07 kg higher for test-day milk yield （P 〈 0.05）, 0.13 kg for fat yield （P 〈 0.01） and 0.07 kg for protein yield （P 〈 0.05） compared with animals having CC and CT genotypes. Definitely, the further research should be conducted to search this gene intensively with larger samples to identify polymorphism and any association between the economically important traits and genotypic class in Holstein cows. Finally, based on the findings, it was concluded that STATI gene might be used as a potential candidate gene to improve milk yield and milk fat and protein contents in dairy cows breeding programs.展开更多
A protein conjugate of streptomycin （streptomycin-bovine serum albumin （BSA） conjugate） was prepared and used as immunogen to produce monoclonal antibodies （MAb）. One hybridoma secreting anti-streptomycin MAb wa...A protein conjugate of streptomycin （streptomycin-bovine serum albumin （BSA） conjugate） was prepared and used as immunogen to produce monoclonal antibodies （MAb）. One hybridoma secreting anti-streptomycin MAb was obtained and then used to produce MAb. The MAb named 13H5 showed the 50% maximal inhibitory concentra- tion （IC50） value of 4.65 ng/ml and the IC20value of 0.21 ng/ml in phosphate buffered saline （PBS）. At optimum con- ditions, an indirect competitive enzyme-linked immunosorbent assay （ELISA） and a colloidal gold-based immuno- chromatographic assay （CGIA） were developed and applied to detect streptomycin residues in milk and swine urine samples. The developed ELISA showed that the minimum detection limit was 2.0 and 1.9 ng/ml for milk and swine urine samples, respectively, without obvious cross-reactivity to other tested antibiotics except dihydrostreptomycin which gave a 118.32% cross reaction value. Milk and swine urine samples spiked with streptomycin at 10, 50, 100 and 200 ng/rnl were analyzed by the established ELISA. The mean recovery of streptomycin was from 81.9% to 105.5% and from 84.3% to 92.2% for milk and swine urine, respectively. The optimized CGIA showed that the minimum de- tection limit was 20.0 ng/ml for milk and swine urine samples. The results of spiked analysis and specific analysis demonstrate that the CGIA could be applicable for screening milk and swine urine samples for the presence of streptomycin residues on-site. The established ELISA and CGIA allow the rapid, low-cost, and sensitive determination of streptomycin residues in food samples.展开更多
文摘为了克服牛奶质量检测方法存在的检测设备昂贵、分析周期长、难以在日常生活中应用等问题,分析并验证射频识别(Radio Frequency Identification,RFID)技术进行牛奶质量检测的方法,设计了基于RFID标签天线的牛奶质量传感器,通过仿真进行性能优化以及实验验证,分析不同介电参数的牛奶对RFID谐振频率的影响,进而根据频率的偏移量判断牛奶是否变质。设计的天线最大近场增益达到74.877 0dB,耦合时的辐射效率达到了95.1%.在用于牛奶检测方面,优化的天线比普通商用天线具有更高的灵敏度。
文摘This paper presents a process which allows the determination of milk adulteration by water. Considering the limitations of the reference method （cryoscopy）, the proposed methodology matches electrical conductivity and cryoscopy index measurements. Based on statistical procedures, a linear descriptive model is achieved to quantify adulteration of milk with water. It is also shown that electrical conductivity measurements allow the detection of adulteration with water and sodium chloride.
基金Supported by National Natural Science Foundation of China(No.30170261)the 10th Five-Year Plan of China(No.2004BA706B12).
文摘To compare mid-infrared(MIR)and near-infrared(NIR)spectroscopies for the determination of the fat and protein contents in milk,the same sample sets with varying concentrations of fat and protein were measured in the MIR range of 3 200-700 cm-1 and NIR range of 9 000-4 000 cm-1.The spectral features in the two regions were analyzed.The MIR spectra of milk were characteristic due to the MIR inherent molecular specificity,whereas the NIR spectra were relatively characterless due to the NIR low selectivity.Partial least squares(PLS)regression models for fat and protein were developed by using both MIR and NIR spectra.MIR data with no pretreatment gave better results than NIR data.The square correlation coefficient(R2)and the root mean square error of prediction(RMSEP)were 0.98 and 0.10 g/dL for fat and 0.97 and 0.11 g/dL for protein.With NIR techniques,satisfactory results were not obtained with raw data.However,NIR data after pretreatment gave similarly good results to the ones using MIR method.This paper indicates that either of the MIR and NIR spectral methods is reliable for the determination of the fat and protein contents.
文摘The purpose of this paper is double： first to measure the natural radioactivity in any food by detecting the omnipresent radiation of ^40K, and then to use this date to obtain the concentration of the important mineral nutrient K, by dividing the specific activity of the sample （Bq/g） by the specific activity of elementary K （31.19 Bq/g）. The method implies the γ rays detection emitted by natural ^40K-^40Ar present in foodstuff, by using a 3 × 3＂ low background NaI （TI） scintillation detector coupled to a PC charged with the Maestro Program. When this detection system is calibrated in order to determine its efficiency for 1461 KeV γrays emitted during ^40K-^40Ar decaying, it is possible to measure easily and with enough precision the specific activity due to natural ^40K present in any foodstuff. Once this figure is divided by the constant value of ^40K specific activity of elementary K, the concentration of this element in the foodstuff sample is established. This paper describes the procedure and shows the results obtained with four basic diet products： milk （powdered, light and cream）, eggs （yellow and white）, wheat （whole and refined flour） and corn （whole and cornstarch flour）. The possible application of this study seems to be to establish the necessary threshold to evaluate the importance of any possible radioactive contamination, as well as to measure the concentration of the mineral nutrient K in foodstuff, by means of an easy, practicable method. Also, it might be useful as an experiment for teaching purposes.
文摘The main purpose of this study was to detect an association of cytoplasmic signal transducers and activators of transcription-1 （STAT1） with milk production traits in 472 Holstein and 283 Jersey cattle breeds of Turkey. This gene, located on chromosome 2, was chosen due to its role on development of mammary gland. A polymorphism of selected 314 bp allele fragment was detected by the restriction fragment length polymorphism analysis of polymerase chain reaction-amplified fragments （PCR-RFLP） method and also confirmed by DNA sequencing. The association tests were conducted between STAT1 genotypes and some economically important dairy traits. The genotypes for C/T as a single nucleotide polymorphism （SNP） were identified at interval 60 cM to 63 cM. The effects of STAT1 gene on milk production traits were not significant in Holstein cows, although animals with CT genotypes showed fairly close to significant value for the corrected 305 d milk yield. However, Jersey cows with/7＂ genotype were 2.07 kg higher for test-day milk yield （P 〈 0.05）, 0.13 kg for fat yield （P 〈 0.01） and 0.07 kg for protein yield （P 〈 0.05） compared with animals having CC and CT genotypes. Definitely, the further research should be conducted to search this gene intensively with larger samples to identify polymorphism and any association between the economically important traits and genotypic class in Holstein cows. Finally, based on the findings, it was concluded that STATI gene might be used as a potential candidate gene to improve milk yield and milk fat and protein contents in dairy cows breeding programs.
基金Project (No.2007C22047) supported by the Program of Science and Technology of Zhejiang Province,China
文摘A protein conjugate of streptomycin （streptomycin-bovine serum albumin （BSA） conjugate） was prepared and used as immunogen to produce monoclonal antibodies （MAb）. One hybridoma secreting anti-streptomycin MAb was obtained and then used to produce MAb. The MAb named 13H5 showed the 50% maximal inhibitory concentra- tion （IC50） value of 4.65 ng/ml and the IC20value of 0.21 ng/ml in phosphate buffered saline （PBS）. At optimum con- ditions, an indirect competitive enzyme-linked immunosorbent assay （ELISA） and a colloidal gold-based immuno- chromatographic assay （CGIA） were developed and applied to detect streptomycin residues in milk and swine urine samples. The developed ELISA showed that the minimum detection limit was 2.0 and 1.9 ng/ml for milk and swine urine samples, respectively, without obvious cross-reactivity to other tested antibiotics except dihydrostreptomycin which gave a 118.32% cross reaction value. Milk and swine urine samples spiked with streptomycin at 10, 50, 100 and 200 ng/rnl were analyzed by the established ELISA. The mean recovery of streptomycin was from 81.9% to 105.5% and from 84.3% to 92.2% for milk and swine urine, respectively. The optimized CGIA showed that the minimum de- tection limit was 20.0 ng/ml for milk and swine urine samples. The results of spiked analysis and specific analysis demonstrate that the CGIA could be applicable for screening milk and swine urine samples for the presence of streptomycin residues on-site. The established ELISA and CGIA allow the rapid, low-cost, and sensitive determination of streptomycin residues in food samples.
