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基于Delphi多线程技术的数据下载
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作者 刘智玲 黄皎 +2 位作者 陆晓春 吴万涛 李力刚 《河海大学常州分校学报》 2007年第3期47-50,共4页
通过阐述多线程技术的特点,分析了多线程间的线程同步机制.根据Delphi在数据库操作方面的优势,采用Delphi多线程技术开发能向多台下位机并行下载数据的程序.重点讨论了使用TNMUDP控件发送和接收数据的方法,提出了采用检测重传机制保证... 通过阐述多线程技术的特点,分析了多线程间的线程同步机制.根据Delphi在数据库操作方面的优势,采用Delphi多线程技术开发能向多台下位机并行下载数据的程序.重点讨论了使用TNMUDP控件发送和接收数据的方法,提出了采用检测重传机制保证数据下载可靠性的方法,提高了数据传输的效率,降低了传输时延. 展开更多
关键词 Delphi多线程技术 线程同步 TNMUDP 检测重传
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Expression of Recombinant Protein Bovine Prion pCIp264 in COS-7 Cells and Its Detection
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作者 Yaozhong Ding Yongsbeng Liu Wenqian Liu Yanping Ma Meng Wang Shenghai Yang Jie Zhang 《Journal of Life Sciences》 2010年第5期30-36,共7页
Bovine spongiform encephalopathy (BSE) is thought to be caused by prions initially derived from sheep scrapie, and epidemiological studies suggest that new viriant form of CJD manifest in Great Britain may be caused... Bovine spongiform encephalopathy (BSE) is thought to be caused by prions initially derived from sheep scrapie, and epidemiological studies suggest that new viriant form of CJD manifest in Great Britain may be caused by BSE prions. PrP^Sc is thought to pathogenic factor of transmissible spongiform encephalopathy (TSE), which invariably involve a post-translational modification process of PrPc encoded by the host euchromosome PrP gene during the period it converted into the pathogenic form (PrP^Sc), PrP is nomal cellular protein which has been found in both neuronal and nonneuronal tissues. Since the crucial infectious event in protein-transmitted diseases is an induced misfolding of prion proteins (PrP^c) catalyzed by already misfolded PrP^Sc, it is of high importance that such collisions are enhanced by two-dimensional diffusion in cell membranes is of high importance compared to three-dimensional diffusion in solution. The level of PrP mRNA in brain is higher than other tissue, but purification of PrPc from rodent has been difficult. To understand the formation of PrP^Sc, it seemed useful to develop a system for produced a large quantities of PrPc since there is no nature source of PrP^c. The pCI-neo mammalian expression vector contains the neomycin phosphtransferase gene which serves as a marker for the selection of stable transfected cells with G418. COS-7 cells constitutively express simian viruse 40 (SV40) T-antigen and support replication of expression plasmids containing the SV40 origin of replication, amplifying the introduced expression cassettes, now become important routine of expression a large number of heterologous gene products. In this paper, the authors used pCI-neo vector to construct a recombnant pCIp264 (cotains mPrP, N-signalpeptide and C-GPI anchor) plasmid to express it in the COS-7 cells and meanwhile detect the expression fusion using IN-ELISA, IN-IFA and western blot, and obtain some approximative nature PrPc. 展开更多
关键词 Bovine prion protein (boprp) COS-7 cells indirect immunofluorescence assay (IFA)
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Recent Advancement in Optical Fiber Sensing for Aerospace Composite Structures 被引量:5
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作者 Shu MINAKUCHI Nobuo TAKEDA 《Photonic Sensors》 SCIE EI CAS 2013年第4期345-354,共10页
Optical fiber sensors have attracted considerable attention in health monitoring of aerospace composite structures. This paper briefly reviews our recent advancement mainly in Brillouin-based distributed sensing. Dama... Optical fiber sensors have attracted considerable attention in health monitoring of aerospace composite structures. This paper briefly reviews our recent advancement mainly in Brillouin-based distributed sensing. Damage detection, life cycle monitoring and shape reconstruction systems applicable to large-scale composite structures are presented, and new technical concepts, "smart crack arrester" and "hierarchical sensing system", are described as well, highlighting the great potential of optical fiber sensors for the structural health monitoring (SHM) field. 展开更多
关键词 Carbon fiber reinforced plastic (CFRP) Brillouin-based system fiber Bragg grating (FBG)
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Porous silicon biosensor for detection of variable domain of heavy-chain of HCAb antibody
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作者 张红燕 吕小毅 +2 位作者 贾振红 李江伟 张富春 《Optoelectronics Letters》 EI 2012年第2期81-83,共3页
In this paper, we produce porous silicon (PSi) by electrochemical etching, and it is the first time to evaluate the performance of label-free porous silicon biosensor for detection of variable domain of heavy chain ... In this paper, we produce porous silicon (PSi) by electrochemical etching, and it is the first time to evaluate the performance of label-free porous silicon biosensor for detection of variable domain of heavy chain of heavy-chain antibody (VHH). The binding of hen egg white lysozyme (HEWL) and VHH causes a red shift in the reflection spectrum of the biosensor. The red shift is proportional to the VHH concenlration in the range from 14 gg.ml-I to 30 pg.ml~ with a detection limit of 0.648 ng.ml~. The research is useful for the development of label-free biosensor applied in the rapid and sensitive determination of small molecules. 展开更多
关键词 ANTIBODIES Chemical detection Doppler effect Porous silicon
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