Objective To develop an alternative method for assessment of gene delivery systems in vivo.Methods Mouse primary spleen lymphocytes were genetically modified in vitro by a retroviral vector harboring a Gaussia lucifer...Objective To develop an alternative method for assessment of gene delivery systems in vivo.Methods Mouse primary spleen lymphocytes were genetically modified in vitro by a retroviral vector harboring a Gaussia luciferase(Gluc) expression cassette.After implantation of these cells into recipient mice,the expression of Gluc was detected in whole blood or plasma collected.Results As little as 10 μL whole blood drawn from the recipient mice could guarantee prompt reading of Gluc activity with a luminometer.And the reading was found in good correlation with the number of genetically modified spleen lymphocytes implanted to the mice.Conclusions Gluc may be useful as an in vivo reporter for gene therapy researches,and Gluc blood assay could provide an alternative method for assessment of gene delivery systems in vivo.展开更多
Using optical fiber fluorescent technology,a new method for measuring alga concentration in water is presented. The system can realize on line measurement for alga concentration using He Ne laser as the light source. ...Using optical fiber fluorescent technology,a new method for measuring alga concentration in water is presented. The system can realize on line measurement for alga concentration using He Ne laser as the light source. It can also effectively detect weak signals. The system with a passive sensor head has such advantages as simple structure, high sensitivity and high accuracy. It has been demonstrated that this system can be used to monitor water quality and can also be used to survey some matter.展开更多
The determination of the effective minimum detectable activity （MDA） of radionuclides by a detection system plays an im- portant role in environmental radiation monitoring. In this study, the responses of an NaI（TI...The determination of the effective minimum detectable activity （MDA） of radionuclides by a detection system plays an im- portant role in environmental radiation monitoring. In this study, the responses of an NaI（TI） airborne γ ray spectrometry （AGRS） system to different radionuclides （137Cs and 131I） were investigated using the Monte Carlo technique. The MDA values were determined under different conditions according to the counting spectra obtained from the Monte Carlo simulation. The equivalent mass thickness method was applied to the Monte Carlo modeling for monitoring ground radiation to reduce sta- tistical uncertainty. The secondary source method was used to monitor both air and ground radiation. A quadratic relationship was found between the MDA and activity concentration. An exponential relationship was found between the MDA and altitude The MDA of a specific radionuclide from external detectors was found to be superior to that obtained from internal detectors under the same conditions. The MDA values in an NaI（Tl） AGRS system under different conditions can be estimated based on the results of this study.展开更多
基金Supported by National High Technology Research and Development Program of China (863 Program) (2007AA021206,2007AA021106)
文摘Objective To develop an alternative method for assessment of gene delivery systems in vivo.Methods Mouse primary spleen lymphocytes were genetically modified in vitro by a retroviral vector harboring a Gaussia luciferase(Gluc) expression cassette.After implantation of these cells into recipient mice,the expression of Gluc was detected in whole blood or plasma collected.Results As little as 10 μL whole blood drawn from the recipient mice could guarantee prompt reading of Gluc activity with a luminometer.And the reading was found in good correlation with the number of genetically modified spleen lymphocytes implanted to the mice.Conclusions Gluc may be useful as an in vivo reporter for gene therapy researches,and Gluc blood assay could provide an alternative method for assessment of gene delivery systems in vivo.
文摘Using optical fiber fluorescent technology,a new method for measuring alga concentration in water is presented. The system can realize on line measurement for alga concentration using He Ne laser as the light source. It can also effectively detect weak signals. The system with a passive sensor head has such advantages as simple structure, high sensitivity and high accuracy. It has been demonstrated that this system can be used to monitor water quality and can also be used to survey some matter.
基金supported by the National Defense Basic Scientific Research Project(Grant No.B2520133077)National High-tech R&D Program of China("863"Program)(Grant No.2012AA061803)
文摘The determination of the effective minimum detectable activity （MDA） of radionuclides by a detection system plays an im- portant role in environmental radiation monitoring. In this study, the responses of an NaI（TI） airborne γ ray spectrometry （AGRS） system to different radionuclides （137Cs and 131I） were investigated using the Monte Carlo technique. The MDA values were determined under different conditions according to the counting spectra obtained from the Monte Carlo simulation. The equivalent mass thickness method was applied to the Monte Carlo modeling for monitoring ground radiation to reduce sta- tistical uncertainty. The secondary source method was used to monitor both air and ground radiation. A quadratic relationship was found between the MDA and activity concentration. An exponential relationship was found between the MDA and altitude The MDA of a specific radionuclide from external detectors was found to be superior to that obtained from internal detectors under the same conditions. The MDA values in an NaI（Tl） AGRS system under different conditions can be estimated based on the results of this study.
