Group testing is a method that can be used to estimate the prevalence of rare infectious diseases,which can effectively save time and reduce costs compared to the method of random sampling.However,previous literature ...Group testing is a method that can be used to estimate the prevalence of rare infectious diseases,which can effectively save time and reduce costs compared to the method of random sampling.However,previous literature only demonstrated the optimality of group testing strategy while estimating prevalence under some strong assumptions.This article weakens the assumption of misclassification rate in the previous literature,considers the misclassification rate of the infected samples as a differentiable function of the pool size,and explores some optimal properties of group testing for estimating prevalence in the presence of differential misclassification conforming to this assumption.This article theoretically demonstrates that the group testing strategy performs better than the sample by sample procedure in estimating disease prevalence when the total number of sample pools is given or the size of the test population is determined.Numerical simulation experiments were conducted to evaluate the performance of group tests in estimating prevalence in the presence of dilution effect.展开更多
On the basis of strict mathematical description about Failure_Free Period Life Test (FFPLT), the statistical properties of the tests and optimal confidence limit of the parameter are discussed in detail and correspond...On the basis of strict mathematical description about Failure_Free Period Life Test (FFPLT), the statistical properties of the tests and optimal confidence limit of the parameter are discussed in detail and corresponding calculating formulae are found out.展开更多
Research on learning difficulties in mathematics adopts one of two distinct perspectives. According to the first, learning difficulties are due to the intrinsic characteristics of the student. For supporters of the se...Research on learning difficulties in mathematics adopts one of two distinct perspectives. According to the first, learning difficulties are due to the intrinsic characteristics of the student. For supporters of the second perspective, those difficulties result from the interaction between the student and the school system. The objective of this study is to test the validity of these two perspectives in interpreting learning difficulties in mathematics among at-risk students. To this end, we collaborated with normally achieving （undiagnosed） and at-risk students. Results show that the second perspective is better suited to the interpretation of learning difficulties in mathematics students展开更多
Objective：The aim of this study was to prepare monoclonal antibody against P53, a kind of tumor suppressor protein,and use the antibody initial y in clinical immunoassay. Methods：Monoclonal antibody was prepared and...Objective：The aim of this study was to prepare monoclonal antibody against P53, a kind of tumor suppressor protein,and use the antibody initial y in clinical immunoassay. Methods：Monoclonal antibody was prepared and identified via the classic protocol of monoclonal antibody preparation. Identified monoclonal antibodies were purified by af inity chro-matography. Antibody titer was determined by enzyme linked immunosorbent assay （ELISA）. The specific binding activity of antibody was detected by Western blotting and immunohistochemistry. Results：Three strains of monoclonal antibodies named 1P15, 2P37 and 3P40 were obtained and purified by af inity chromatography. The purity of antibodies was higher than 90%. The titers of antibodies were more than 1：6000. Western blot and immunohistochemistry assay showed that the specific antibody can combine with endogenous P53 protein in the tumor celllines and determine the expression of P53 in tumor tis-sue. Conclusion：Three strains of monoclonal antibodies with high af inity to P53 were successful y established, which can be used for detecting the expression of P53 in tumor cells or tissue.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.72091212).
文摘Group testing is a method that can be used to estimate the prevalence of rare infectious diseases,which can effectively save time and reduce costs compared to the method of random sampling.However,previous literature only demonstrated the optimality of group testing strategy while estimating prevalence under some strong assumptions.This article weakens the assumption of misclassification rate in the previous literature,considers the misclassification rate of the infected samples as a differentiable function of the pool size,and explores some optimal properties of group testing for estimating prevalence in the presence of differential misclassification conforming to this assumption.This article theoretically demonstrates that the group testing strategy performs better than the sample by sample procedure in estimating disease prevalence when the total number of sample pools is given or the size of the test population is determined.Numerical simulation experiments were conducted to evaluate the performance of group tests in estimating prevalence in the presence of dilution effect.
文摘On the basis of strict mathematical description about Failure_Free Period Life Test (FFPLT), the statistical properties of the tests and optimal confidence limit of the parameter are discussed in detail and corresponding calculating formulae are found out.
文摘Research on learning difficulties in mathematics adopts one of two distinct perspectives. According to the first, learning difficulties are due to the intrinsic characteristics of the student. For supporters of the second perspective, those difficulties result from the interaction between the student and the school system. The objective of this study is to test the validity of these two perspectives in interpreting learning difficulties in mathematics among at-risk students. To this end, we collaborated with normally achieving （undiagnosed） and at-risk students. Results show that the second perspective is better suited to the interpretation of learning difficulties in mathematics students
基金Supported by grants from the National Natural Science Foundation of China(No.30973562)National Basic Research Program of China(No.2010CB933904)
文摘Objective：The aim of this study was to prepare monoclonal antibody against P53, a kind of tumor suppressor protein,and use the antibody initial y in clinical immunoassay. Methods：Monoclonal antibody was prepared and identified via the classic protocol of monoclonal antibody preparation. Identified monoclonal antibodies were purified by af inity chro-matography. Antibody titer was determined by enzyme linked immunosorbent assay （ELISA）. The specific binding activity of antibody was detected by Western blotting and immunohistochemistry. Results：Three strains of monoclonal antibodies named 1P15, 2P37 and 3P40 were obtained and purified by af inity chromatography. The purity of antibodies was higher than 90%. The titers of antibodies were more than 1：6000. Western blot and immunohistochemistry assay showed that the specific antibody can combine with endogenous P53 protein in the tumor celllines and determine the expression of P53 in tumor tis-sue. Conclusion：Three strains of monoclonal antibodies with high af inity to P53 were successful y established, which can be used for detecting the expression of P53 in tumor cells or tissue.
