Cotton leaf curl virus (CLCuV) belongs to the subgroup III of geminiviruses with single strand DNA genome. Study demonstrated that the bidirectional promoter of CLCuV had activity in Agrobacterium tumefaciens (Smith e...Cotton leaf curl virus (CLCuV) belongs to the subgroup III of geminiviruses with single strand DNA genome. Study demonstrated that the bidirectional promoter of CLCuV had activity in Agrobacterium tumefaciens (Smith et Townsend) Conn. This is the first report for the activity of the bidirectional promoter of geminivirus in A. tumefaciens. Results showed that the activity of the complementary sense promoter was stronger than that of virion sense promoter, and was detected 2-fold higher than that of CaMV 35S promoter in A. tumefaciens. Moreover, the promoter 5' deletion analysis indicated that the mean GUS activity driven by a 287 nucleotides complementary sense promoter fragment (from-287 to the translation initiation site) is 4 times higher than that driven by the whole complementary sense promoter in A. tumefaciens. This result suggested that there might exist negative regulatory elements in this deleted fragment. The function of other cis-elements included in CLCuV complementary sense promoter was also discussed in this paper.展开更多
Sulphuric acid activated immature Gossypium hirsutum seed(AIGHS) was prepared to biosorbe basic violet 10(BV10) from aqueous solutions.Methylene blue number,iodine number and Brunauer-Emmett-Teller surface analysis in...Sulphuric acid activated immature Gossypium hirsutum seed(AIGHS) was prepared to biosorbe basic violet 10(BV10) from aqueous solutions.Methylene blue number,iodine number and Brunauer-Emmett-Teller surface analysis indicated that the AIGHS were hetero-porous.Boehm titrations and Fourier-transform infrared spectra demonstrated the chemical heterogeneity of the AIGHS surface.Batch biosorption studies were used to examine the effects of process parameters in the following range:pH 2-12,temperature 293-313 K,contact time 1-5 h and initial concentration 200-600 mg·L^(-1).The matching of equilibrium data with the Langmuir-Freundlich form of isotherms indicated that the BV10 was adsorbed via chemisorption and pore diffusion.Kinetic investigation indicated multiple order chemisorption through an Avrami kinetic model.Film diffusion controlled the rate of BV10 biosorption onto AIGHS.The spontaneous and endothermic nature of sorption was corroborated by thermodynamic study.Continuous biosorption experiments were performed using a fixed-bed column and the influence of operating parameters was explored for different ranges of initial concentration 100-300 mg·L^(-1),bed height 5-10 cm,and flow rate 2.5-4.5 ml·min^(-1).A dose response model accurately described the fixed-bed biosorption data.An external mass transfer correlation was formulated explaining BV10-AIGHS sorption.展开更多
The green mirid bug Apolygus lucorum is an agricultural pest that is known to cause damage to more than 150 plant species. Here, we report the transcriptomes ofA. lucorum at three different developmental stages （the ...The green mirid bug Apolygus lucorum is an agricultural pest that is known to cause damage to more than 150 plant species. Here, we report the transcriptomes ofA. lucorum at three different developmental stages （the second and fifth instar nymphs and adults）. A total of 98,236 unigenes with an average length of 1,335nt was obtained, of which 50,640 were annotated, including those encoding digestive enzymes and cytochrome P450s. Comparisons with cotton bollworm and cotton aphid transcriptomes revealed distinct features ofA. lucorum as a mesophyll feeder. The gene expression dynamics varied during development from young nymphs to adults. The high-quality transcriptome data and the gene expression dynamics reported here provide valuable data for a more comprehensive understanding of the physiology and development of mirid bugs, and for mining targets for their control.展开更多
文摘Cotton leaf curl virus (CLCuV) belongs to the subgroup III of geminiviruses with single strand DNA genome. Study demonstrated that the bidirectional promoter of CLCuV had activity in Agrobacterium tumefaciens (Smith et Townsend) Conn. This is the first report for the activity of the bidirectional promoter of geminivirus in A. tumefaciens. Results showed that the activity of the complementary sense promoter was stronger than that of virion sense promoter, and was detected 2-fold higher than that of CaMV 35S promoter in A. tumefaciens. Moreover, the promoter 5' deletion analysis indicated that the mean GUS activity driven by a 287 nucleotides complementary sense promoter fragment (from-287 to the translation initiation site) is 4 times higher than that driven by the whole complementary sense promoter in A. tumefaciens. This result suggested that there might exist negative regulatory elements in this deleted fragment. The function of other cis-elements included in CLCuV complementary sense promoter was also discussed in this paper.
文摘Sulphuric acid activated immature Gossypium hirsutum seed(AIGHS) was prepared to biosorbe basic violet 10(BV10) from aqueous solutions.Methylene blue number,iodine number and Brunauer-Emmett-Teller surface analysis indicated that the AIGHS were hetero-porous.Boehm titrations and Fourier-transform infrared spectra demonstrated the chemical heterogeneity of the AIGHS surface.Batch biosorption studies were used to examine the effects of process parameters in the following range:pH 2-12,temperature 293-313 K,contact time 1-5 h and initial concentration 200-600 mg·L^(-1).The matching of equilibrium data with the Langmuir-Freundlich form of isotherms indicated that the BV10 was adsorbed via chemisorption and pore diffusion.Kinetic investigation indicated multiple order chemisorption through an Avrami kinetic model.Film diffusion controlled the rate of BV10 biosorption onto AIGHS.The spontaneous and endothermic nature of sorption was corroborated by thermodynamic study.Continuous biosorption experiments were performed using a fixed-bed column and the influence of operating parameters was explored for different ranges of initial concentration 100-300 mg·L^(-1),bed height 5-10 cm,and flow rate 2.5-4.5 ml·min^(-1).A dose response model accurately described the fixed-bed biosorption data.An external mass transfer correlation was formulated explaining BV10-AIGHS sorption.
基金supported by the Strategic Priority Research Program of Chinese Academy of Sciences(XDB11030000)the Ministry of Agriculture of China(2014ZX08009001-009)
文摘The green mirid bug Apolygus lucorum is an agricultural pest that is known to cause damage to more than 150 plant species. Here, we report the transcriptomes ofA. lucorum at three different developmental stages （the second and fifth instar nymphs and adults）. A total of 98,236 unigenes with an average length of 1,335nt was obtained, of which 50,640 were annotated, including those encoding digestive enzymes and cytochrome P450s. Comparisons with cotton bollworm and cotton aphid transcriptomes revealed distinct features ofA. lucorum as a mesophyll feeder. The gene expression dynamics varied during development from young nymphs to adults. The high-quality transcriptome data and the gene expression dynamics reported here provide valuable data for a more comprehensive understanding of the physiology and development of mirid bugs, and for mining targets for their control.
