棉籽饼中棉酚降解菌株的分离及脱毒效果初探

从棉田土壤中分离筛选对棉酚具有降解作用菌株,得到1株具有较高降解活性的菌株,命名为M12。经鉴定,初步确定菌株M12为鲁氏毛霉(Mucor rourianus),进一步脱毒研究表明,菌株M12具有降解棉籽饼中游离棉酚的能力,降解率在80%以上,具有进一步开发的潜力。

高效降解棉酚菌株的筛选鉴定及毒性试验

为了筛选一株能高效降解棉籽饼中棉酚毒素的菌株,以棉酚为唯一碳源,从棉田土壤中分离筛选对棉酚具有降解作用的菌株,得到具有较高降解活性的菌株M-9,其降解率达到94.74%,对其进行了菌株形态观察和生理生化鉴定并结合其16S rDNA序列,初步确定其为枯草芽孢杆菌(Bacillus subtilis)。通过对M-9菌株的脱毒效果研究表明,其具有降解棉籽饼中游离棉酚的能力,脱毒后棉籽饼中游离棉酚的含量降至33.18 mg/kg,符合中国国家标准饲料卫生标准规定(GB13078—2001):饲料原料棉籽饼中游离棉酚含量≤1200 mg/kg。并进行毒性饲喂试验,从而确定菌株M-9处理后的棉籽饼粉对小白鼠无毒性。

高效降解棉酚菌株的选育及脱毒条件的研究

From mildewed cottonseed cake and stock cultUres of mold and yeast We select more than ten strains of yeastS and molds which can degrade cotton Phenol. At last we got four strains which can degrade cotton phenol highly effeted after mutagenized by physical and chemical factors and induced by cotton Phenol. They belong to Candida tropicalis, Torulopsis candida, Aspergillus flavus and ASPergillus niger. By small and medium size fermenfations, the content of dissociated cottom Phenol all reach safe criterion (the lowest dissociated cotton phenol content is 220mg/ in the defoxicated cottonseed cake), compared with FeSO4 method, defoxicated cottonseed cake looks and taStes well and the content of protein and amino acid are highly enhanced as will.