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QuEChERS-液相色谱-串联质谱法测定棕榈原油中敌草快等6种农药残留 被引量:19
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作者 王连珠 李晓莲 +3 位作者 方恩华 陈泳 王登飞 徐敦明 《色谱》 CAS CSCD 北大核心 2016年第7期686-691,共6页
建立了QuEChERS-液相色谱-串联质谱同时测定棕榈原油中敌草快、乐果、倍硫磷、倍硫磷亚砜、倍硫磷砜、氟吡甲禾灵残留量的方法。以回收率及基质效应为考察指标,评估了乙二胺-N-丙基硅烷(PSA)/C18混合物、C18键合锆胶(Z-Sep+)/C18... 建立了QuEChERS-液相色谱-串联质谱同时测定棕榈原油中敌草快、乐果、倍硫磷、倍硫磷亚砜、倍硫磷砜、氟吡甲禾灵残留量的方法。以回收率及基质效应为考察指标,评估了乙二胺-N-丙基硅烷(PSA)/C18混合物、C18键合锆胶(Z-Sep+)/C18混合物、Z-Sep+、C18 4种分散吸附剂的净化效果,结果表明Z-Sep+/C18的净化效果最佳。样品采用含1%(v/v)乙酸的甲醇溶液提取,Z-Sep+/C18分散固相萃取净化;液相色谱以Atlantis T3色谱柱梯度洗脱分离,质谱分析采用电喷雾正离子电离、多反应监测模式,以基质匹配校准曲线外标法进行定量。6种农药在3个添加水平(10、20、250μg/kg)下的回收率范围为70.0%~97.9%,相对标准偏差范围为2.6%~10.2%;6种农药的定量限(S/N≥10)为0.1~2.5μg/kg。该方法简便、灵敏、准确、环保,适用于棕榈原油中上述6种农药残留的定性、定量分析。 展开更多
关键词 QUECHERS 液相色谱-串联质谱 敌草快 农药残留 棕榈原油 氧化锆
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Utilization of Fungi for the Biological Control of Insect Pests and Ganoderma Disease in the Indonesian Oil Palm Industry
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作者 Hari Priwiratama Agus Susanto 《Journal of Agricultural Science and Technology(A)》 2014年第2期103-111,共9页
Biological control is usually the first choice of control and prevention method for integrated pest management (IPM) strategies and has now been widely implemented by Indonesian oil palm plantations. Entomopathogeni... Biological control is usually the first choice of control and prevention method for integrated pest management (IPM) strategies and has now been widely implemented by Indonesian oil palm plantations. Entomopathogenic fungus, i.e., Metarhizium anisopliae, Cordyceps militaris and Beauveria bassiana have been demonstrated to control renowned pests of oil palm. Metarhizium has been used to control Oryctes larvae and the mortality has ranged from 91.67% to 100% in laboratory and 7.4% to 88.75% in the field. Metarhizium has been applied in combination with a termite baiting system (TBS) to control termites in the field for preventive and curative action as well. In many oil palm plantations in Indonesia, Cordyceps has been used to reduce the field moth population ofSetothosea asigna. Application of Cordyceps within the oil palm circle was able to infect S. asigna pupae up to 80%. Meanwhile, Beauveria in an effervescent formulation was demonstrated to have better efficacy on Darna trima larvae. A significant finding on the biological control of basal stem rot disease (Ganoderma) was the isolation of Trichoderma sp. and Gliocladium sp.. The efficacy was conducted with promising result and techniques on the application of Triehoderma have been developed, i.e., hole-in-hole system, surgery and a mounding method. However, as the roots developed, Trichoderma was no longer able to protect the palm from Ganoderma. In spite of that, the use of Trichoderma still prolonged the life ofoil palms by up to 2-3 years. Another fungi belonging to vesicular arbuscular mychorrhiza (VAM) has been developed to control Ganoderma. The efficacy in the nursery showed promising results and the Ganoderma incidence remained low compared to the untreated control. Large scale field trials are ongoing. Challenges on the implementation of biological control in oil palm plantations are because of application and availability of biopesticides/natural enemies. Therefore, advances in research on the formulation of biological control agents are still needed. 展开更多
关键词 Entomopathogenic fungus biological control mixture formula GANODERMA VAM
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