[Objective] This study aimed to investigate the effect of inoculation with various rhizobium strains on growth status, phosphorus uptake, yield and quality of soybean plants. [Method] Using Dongnong 42 and Dongnong 46...[Objective] This study aimed to investigate the effect of inoculation with various rhizobium strains on growth status, phosphorus uptake, yield and quality of soybean plants. [Method] Using Dongnong 42 and Dongnong 46 as experimental soybeans, four treatments were designed, including non-inoculated CK group and three treatment groups inoculated respectively with rhizobium strains R2, R, and Rs, to analysis the effects of rhizobium inoculation on growth status, phosphorus uptake, yield and quality of soybean plants. [Result] Inoculation with different rhizobium strains could significantly increase the biomass and phosphorus content of the roots and shoots of Dongnong 42, to be specific, rhizobium strain R2 showed the best ef- fect, followed by Rs, and those of roots were improved greater than the shoots; in- oculation with rhizobium could significantly increase the yield of Dongnong 42, and R5 showed the best effect, but the yield of Dongnong 46 was decreased; after in- oculated with rhizobium, the protein content and fat content of soybean were signifi- cantly increased, and R2 showed the best effect, but the effects on different varieties varied, to be specific, the protein content of Dongnong 42 was significantly in- creased, while the fat content of Dongnong 46 was significantly increased; different rhizobium strains inoculated to different soybean varieties showed different effects. Inoculation with suitable rhizobium can improve plant growth characteristics, promote phosphorus uptake of plants, increase soybean yield, and effectively improve soy- bean quality. [Conclusion] This study provides theoretical basis for solving the prob- lem of soil phosphorus deficiency, increasing soybean yield and improving soybean quality in the future.展开更多
Corn and wheat plants were grown in a nutrient culture solution at four levels of phosphorus (0, 0.12,0.60 and 3.0 mmol L-1) and two levels of cadmium (0 and 4.0 pmol L--1) in greenhouse for a 18-day period.The concen...Corn and wheat plants were grown in a nutrient culture solution at four levels of phosphorus (0, 0.12,0.60 and 3.0 mmol L-1) and two levels of cadmium (0 and 4.0 pmol L--1) in greenhouse for a 18-day period.The concentrations of phosphorus and cadmium in cell wall, cytoplasm and vacuoles of roots and leaveswere examined by cell fractionation techniques. With increasing phosphorus in medium, the contents of Pin cell wall, cytoplasm and vacuoles of corn and wheat roots and leaves increased. The highest content of Pwas observed in cell wall, next in vacuoles, and the lowest in cytoplasm. The wheat subcellular fractions inboth roots and leaves had higher concentrations of phosphorus than those of corn. Increasing phosphorus inmedium significantly inhibited the intracellular Cd accumulation in both species. However, at P concentrationup to 3.0 mmol L--1, the Cd content in cell wall was increased. Increasing phosphorus resulted in reductionof the subcellular Cd content in corn and wheat leaves. Compared with corn, the wheat roots had a higherCd content in the cell wall and vacuoles and a lower in cytoplasm, while in leaf subcellular fractions thewheat cell had a higher Cd content in its vacuoles and a lower one in its cytoplasm. The results indicate thatphosphorus may be involved in sequestration of Cd ionic activity in both cell wall and vacuoles by forminginsoluble Cd phosphate.展开更多
The disadvantages of the long term application of Tilemsi natural phosphate （TNP） on maize （Zea mays L.） production has come out because of its low P availability. Some functional soil microbes, such as phosphate ...The disadvantages of the long term application of Tilemsi natural phosphate （TNP） on maize （Zea mays L.） production has come out because of its low P availability. Some functional soil microbes, such as phosphate dissolving bacteria, have great potential in improvement of P solubility from TNP and P uptake by plants. The present study aimed to isolate and characterize typical phosphate dissolving bacterial strains （Bacillus subtilis） from Malian soils, and investigate their role in P uptake by maize grown in soils amended with TNP. The experimental design was a split plot with three main plots of fertilizers sources, i.e., natural phosphate, commercial fertilizer and without fertilizer, and with seven sub-plots of six microorganisms plus the control. The field experiment results have shown that the maize inoculated with the phosphate dissolving bacteria was improved in seed germination, plant growth, plant production （increase yield by 42%）, grain and aerial dry biomass （P） content of 34% and 64%, respectively. They have also shown that the locally available TNP can be used by the Malians farmers in maize culture and have comparable production to the one obtained with the costly imported commercial phosphate fertilizer, like the complex cereal. The project has provided information for the combined use of the Mali TNP and phosphate dissolving bacteria Bacillus subtilis subsp, subtilis （T）： DSM 10 in improvement of maize production in the country.展开更多
Low temperature is one of the most important environmental stresses that affect plant survival,growth and development.In response to cold stress,hundreds of genes are upor down-regulated in plants[1].The C-repeat bind...Low temperature is one of the most important environmental stresses that affect plant survival,growth and development.In response to cold stress,hundreds of genes are upor down-regulated in plants[1].The C-repeat binding factor（CBF）regulon is the best-understood cold-responsive transcriptional module[1].In Arabidopsis,there are three CBF genes,i.e.,CBF1,CBF2 and CBF3.They are rapidly.展开更多
基金Supported by Doctoral Scientific Research Start-up Project of Mudanjiang Normal University(MSB200912)"12th Five-Year Plan"Educational Science and Research Project of Heilongjiang Association of Higher Education(HGJXH B1110053)Teaching Reform Project of Mudanjiang Normal University(10-XY01067)~~
文摘[Objective] This study aimed to investigate the effect of inoculation with various rhizobium strains on growth status, phosphorus uptake, yield and quality of soybean plants. [Method] Using Dongnong 42 and Dongnong 46 as experimental soybeans, four treatments were designed, including non-inoculated CK group and three treatment groups inoculated respectively with rhizobium strains R2, R, and Rs, to analysis the effects of rhizobium inoculation on growth status, phosphorus uptake, yield and quality of soybean plants. [Result] Inoculation with different rhizobium strains could significantly increase the biomass and phosphorus content of the roots and shoots of Dongnong 42, to be specific, rhizobium strain R2 showed the best ef- fect, followed by Rs, and those of roots were improved greater than the shoots; in- oculation with rhizobium could significantly increase the yield of Dongnong 42, and R5 showed the best effect, but the yield of Dongnong 46 was decreased; after in- oculated with rhizobium, the protein content and fat content of soybean were signifi- cantly increased, and R2 showed the best effect, but the effects on different varieties varied, to be specific, the protein content of Dongnong 42 was significantly in- creased, while the fat content of Dongnong 46 was significantly increased; different rhizobium strains inoculated to different soybean varieties showed different effects. Inoculation with suitable rhizobium can improve plant growth characteristics, promote phosphorus uptake of plants, increase soybean yield, and effectively improve soy- bean quality. [Conclusion] This study provides theoretical basis for solving the prob- lem of soil phosphorus deficiency, increasing soybean yield and improving soybean quality in the future.
文摘Corn and wheat plants were grown in a nutrient culture solution at four levels of phosphorus (0, 0.12,0.60 and 3.0 mmol L-1) and two levels of cadmium (0 and 4.0 pmol L--1) in greenhouse for a 18-day period.The concentrations of phosphorus and cadmium in cell wall, cytoplasm and vacuoles of roots and leaveswere examined by cell fractionation techniques. With increasing phosphorus in medium, the contents of Pin cell wall, cytoplasm and vacuoles of corn and wheat roots and leaves increased. The highest content of Pwas observed in cell wall, next in vacuoles, and the lowest in cytoplasm. The wheat subcellular fractions inboth roots and leaves had higher concentrations of phosphorus than those of corn. Increasing phosphorus inmedium significantly inhibited the intracellular Cd accumulation in both species. However, at P concentrationup to 3.0 mmol L--1, the Cd content in cell wall was increased. Increasing phosphorus resulted in reductionof the subcellular Cd content in corn and wheat leaves. Compared with corn, the wheat roots had a higherCd content in the cell wall and vacuoles and a lower in cytoplasm, while in leaf subcellular fractions thewheat cell had a higher Cd content in its vacuoles and a lower one in its cytoplasm. The results indicate thatphosphorus may be involved in sequestration of Cd ionic activity in both cell wall and vacuoles by forminginsoluble Cd phosphate.
文摘The disadvantages of the long term application of Tilemsi natural phosphate （TNP） on maize （Zea mays L.） production has come out because of its low P availability. Some functional soil microbes, such as phosphate dissolving bacteria, have great potential in improvement of P solubility from TNP and P uptake by plants. The present study aimed to isolate and characterize typical phosphate dissolving bacterial strains （Bacillus subtilis） from Malian soils, and investigate their role in P uptake by maize grown in soils amended with TNP. The experimental design was a split plot with three main plots of fertilizers sources, i.e., natural phosphate, commercial fertilizer and without fertilizer, and with seven sub-plots of six microorganisms plus the control. The field experiment results have shown that the maize inoculated with the phosphate dissolving bacteria was improved in seed germination, plant growth, plant production （increase yield by 42%）, grain and aerial dry biomass （P） content of 34% and 64%, respectively. They have also shown that the locally available TNP can be used by the Malians farmers in maize culture and have comparable production to the one obtained with the costly imported commercial phosphate fertilizer, like the complex cereal. The project has provided information for the combined use of the Mali TNP and phosphate dissolving bacteria Bacillus subtilis subsp, subtilis （T）： DSM 10 in improvement of maize production in the country.
文摘Low temperature is one of the most important environmental stresses that affect plant survival,growth and development.In response to cold stress,hundreds of genes are upor down-regulated in plants[1].The C-repeat binding factor（CBF）regulon is the best-understood cold-responsive transcriptional module[1].In Arabidopsis,there are three CBF genes,i.e.,CBF1,CBF2 and CBF3.They are rapidly.
