[Objective] The study aimed to find a possible way to combat or alleviate the negative effects caused by high temperature and water deficit at the growth stage of peak boll-setting.[Method] With Bt transgenic cotton G...[Objective] The study aimed to find a possible way to combat or alleviate the negative effects caused by high temperature and water deficit at the growth stage of peak boll-setting.[Method] With Bt transgenic cotton GK22 as the test cultivar,a potted experiment was carried out to investigate the effects of the regulation of external substances(the water solutions of pix,urea and their mixture) on the physiological parameters,insecticidal protein content,yield and yield component of cotton plants in artificial climate chambers treated with high temperature and water deficit.[Result] The application of external pix,urea or their mixture was effective in stabilizing the physiological parameters of cotton plants,insecticidal protein content,yield and yield components.Compared with the exclusive application of pix and urea,the mixture of pix and urea played the most effective role in stabilizing the content of chlorophyll,soluble sugar and insecticidal protein,alleviating the increase of the content of free amino acids and proline,and increasing boll number per plant,boll weight and seed cotton yield.[Conclusion] The water solutions of pix,urea or their mixtures can be used to combat or alleviate the stress of high temperature and water deficit if they are sprayed onto cotton plants prior to stress occurrence.展开更多
Sesquiterpenoids are a class of 15-carbon secondary metabolites that play diverse roles in plant adaptation to environment. Cotton plants accumulate a large amount of sesquiterpene aldehydes （including gossypol） as ...Sesquiterpenoids are a class of 15-carbon secondary metabolites that play diverse roles in plant adaptation to environment. Cotton plants accumulate a large amount of sesquiterpene aldehydes （including gossypol） as phytoalexins against pathogens and herbivores. They are stored in pigment glands of aerial organs and in epidermal layers of roots. Several enzymes of goss- ypol biosynthesis pathway have been characterized, including 3-hydroxy-3-methylglutaryl coenzyme A reductase （HMGR） and farnesyl diphosphate synthase （FPS） that catalyze the formation of the precursor farnesyl diphosphate （FPP）, （＋）-6-cadinene synthase （CDN） which is the first enzyme committed to gossypol biosynthesis, and the downstream enzymes of CYP706B 1 and methyltransferase. Expressions of these genes are tightly regulated during cotton plants development and in- duced by jasmonate and fungi elicitors. The transcription factor GaWRKY1 has been shown to be involved in gossypol path- way regulation. Recent development of new genomic platforms and methods and releases of diploid and tetraploid cotton ge- nome sequences will greatly facilitate the elucidation of gossypol biosynthetic pathway and its regulation.展开更多
基金Supported by the National Natural Science Foundation of China(3077127231171483)+1 种基金the Priority Academic Program Development of Jiangsu Higher Education Institutions,Jiangsu Innovation Project for Agriculture Science and Technology [cx(11)2054 ]Jiangsu Agriculture Science and Technology Support Program(SBE2010307)
文摘[Objective] The study aimed to find a possible way to combat or alleviate the negative effects caused by high temperature and water deficit at the growth stage of peak boll-setting.[Method] With Bt transgenic cotton GK22 as the test cultivar,a potted experiment was carried out to investigate the effects of the regulation of external substances(the water solutions of pix,urea and their mixture) on the physiological parameters,insecticidal protein content,yield and yield component of cotton plants in artificial climate chambers treated with high temperature and water deficit.[Result] The application of external pix,urea or their mixture was effective in stabilizing the physiological parameters of cotton plants,insecticidal protein content,yield and yield components.Compared with the exclusive application of pix and urea,the mixture of pix and urea played the most effective role in stabilizing the content of chlorophyll,soluble sugar and insecticidal protein,alleviating the increase of the content of free amino acids and proline,and increasing boll number per plant,boll weight and seed cotton yield.[Conclusion] The water solutions of pix,urea or their mixtures can be used to combat or alleviate the stress of high temperature and water deficit if they are sprayed onto cotton plants prior to stress occurrence.
基金the State Key Basic Research Program of China (2013CB127000)the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB11030300)+1 种基金the National Natural Science Foundation of China (31300255)the Special Fund for Shanghai Landscaping Administration Bureau Program (G142425, F112418).
文摘Sesquiterpenoids are a class of 15-carbon secondary metabolites that play diverse roles in plant adaptation to environment. Cotton plants accumulate a large amount of sesquiterpene aldehydes （including gossypol） as phytoalexins against pathogens and herbivores. They are stored in pigment glands of aerial organs and in epidermal layers of roots. Several enzymes of goss- ypol biosynthesis pathway have been characterized, including 3-hydroxy-3-methylglutaryl coenzyme A reductase （HMGR） and farnesyl diphosphate synthase （FPS） that catalyze the formation of the precursor farnesyl diphosphate （FPP）, （＋）-6-cadinene synthase （CDN） which is the first enzyme committed to gossypol biosynthesis, and the downstream enzymes of CYP706B 1 and methyltransferase. Expressions of these genes are tightly regulated during cotton plants development and in- duced by jasmonate and fungi elicitors. The transcription factor GaWRKY1 has been shown to be involved in gossypol path- way regulation. Recent development of new genomic platforms and methods and releases of diploid and tetraploid cotton ge- nome sequences will greatly facilitate the elucidation of gossypol biosynthetic pathway and its regulation.
