This study was conducted to establish a simple convenient method for calculating crop coefficient, and provide a certain basis for the research of the empirical formula for calculating crop coefficient with plant heig...This study was conducted to establish a simple convenient method for calculating crop coefficient, and provide a certain basis for the research of the empirical formula for calculating crop coefficient with plant height which could be measured conveniently with regional differences, especially for the establishment of accurate irrigation schedule of potato in Yunnan. By the field experiment on potato under the condition of drip irrigation, it was found that the models of plant height with corrected FAO-56-recommended K and measured K were a quartic polynomial and a cubic polynomial, respectively, and the polynomial of potato plant height with measured crop coefficient was simpler with higher degree of fitting; and the differences between the period with the highest change rate of potato plant height and the periods with the greatest FAO-56-recommended K and measured K exhibited a differences of 3 d. In conclusion： In the future study of simple or empirical formula calculation of crop coefficient, plant height should be considered as a main dependent variable in that the calculation result would be closer to the measured crop coefficient with the problem of regional difference existing in the FAO method solved and the formula might be simpler; and the irrigation time of potato should be 3 d earlier than the irrigation time determined according to the corrected FAO-56-recommended crop coefficient, especially in the key water requirement stages of potato.展开更多
[Objective] The aim was to carry out the genetic analysis on plant height of rice（Oryza sativa L.）cultivated in different seasons.[Method] Three rice parents with great difference in plant height including CB1（83.1...[Objective] The aim was to carry out the genetic analysis on plant height of rice（Oryza sativa L.）cultivated in different seasons.[Method] Three rice parents with great difference in plant height including CB1（83.1 cm）,CB4（105.5 cm）and CB7（115.6 cm）were chosen to construct two parental combinations：CB1×CB4 and CB7×CB4,and the corresponding filial generations P1,F1,P2,B1,B2 and F2 were obtained.The 6 populations were planted in middle and late seasons respectively to measure their height traits.The Akaike＇s information criterion（AIC）of the mixed major gene and polygene model was used to indentify the existence of major genes affecting quantitative traits in B1,B2,F2 populations.When the major genes existed,the genetic effects of the major genes and polygenes and their genetic variance were estimated through segregation analysis.[Result] One additive major gene plus additive-dominance polygenes was the most fitted genetic model for the trait in all B1,B2,F2 populations in two planting seasons.The heritability values of the major genes varied from 38.63% to 78.53% and those of polygenes varied from 1.72% to 36.04%,and the total heritability values were 45.52-92.93%.The additive effect d value of the two genetic populations under two planting seasons was-4.56,-9.16,-7.19,and-9.38,respectively,as suggested that additive effect of the major genes would decrease the express of the plant height trait.[Conclusion] The heritability of plant height trait was affected by planting seasons and the combinations clearly as a whole.展开更多
[Objective] The research aimed to study the circadian rhythm of nitrate re- ductase activity （NRA） in plant. [Method] The wheat plants at heading stage were used as the materials for the measurement of dynamic chang...[Objective] The research aimed to study the circadian rhythm of nitrate re- ductase activity （NRA） in plant. [Method] The wheat plants at heading stage were used as the materials for the measurement of dynamic changes of nitrate reductase activity （NRA） within 24 h under the conditions of constant high temperature. [Resulti The fluctuation of NRA in wheat changed greatly from 20：00 pm to 11：00 am. The enzyme activity remained constant, but at 14：00 the enzyme activity was the high- est, higher than all the other time points except the enzyme activity measured at11：00. The enzyme activity was the lowest of 17：00, which was lower than all the other time points except the enzyme activity measured at 2：00. [Conclusion] There were autonomous rhythm changes of NRA in wheat in a certain degree.展开更多
[Objective] The study aimed to find a possible way to combat or alleviate the negative effects caused by high temperature and water deficit at the growth stage of peak boll-setting.[Method] With Bt transgenic cotton G...[Objective] The study aimed to find a possible way to combat or alleviate the negative effects caused by high temperature and water deficit at the growth stage of peak boll-setting.[Method] With Bt transgenic cotton GK22 as the test cultivar,a potted experiment was carried out to investigate the effects of the regulation of external substances(the water solutions of pix,urea and their mixture) on the physiological parameters,insecticidal protein content,yield and yield component of cotton plants in artificial climate chambers treated with high temperature and water deficit.[Result] The application of external pix,urea or their mixture was effective in stabilizing the physiological parameters of cotton plants,insecticidal protein content,yield and yield components.Compared with the exclusive application of pix and urea,the mixture of pix and urea played the most effective role in stabilizing the content of chlorophyll,soluble sugar and insecticidal protein,alleviating the increase of the content of free amino acids and proline,and increasing boll number per plant,boll weight and seed cotton yield.[Conclusion] The water solutions of pix,urea or their mixtures can be used to combat or alleviate the stress of high temperature and water deficit if they are sprayed onto cotton plants prior to stress occurrence.展开更多
Preliminary microarray analysis of cDNA from rice roots revealed an up-regulated transcript that was highly expressed in a five-day iron deficiency treatment. The entire sequence of this gene was determined by bioinfo...Preliminary microarray analysis of cDNA from rice roots revealed an up-regulated transcript that was highly expressed in a five-day iron deficiency treatment. The entire sequence of this gene was determined by bioinformatics analysis. There were no proteins with significant levels of similarity detected in public databases. This novel gene with unknown biological function was designated as OsDPR (dwarf phenotype-related gene). We constructed a stable plant expression vector pCAM- BIA1302-OsDPR::GFP and produced transgenic tobacco plants. The phenotypes suggested that OsDPR restrained the growth of transformed plants. To understand the mechanisms of this suppression effect, cell size and number were compared between transformants and wild-type plants. The cell proliferation rate was lower in OsDPR transgenic BY-2 cells than in wild-type cells, but OsDPR expression did not affect cell size. Moreover, the cell division-related gene CyclinD2.1, which is involved in plant growth, was down-regulated in transgenic tobacco plants. These findings suggested that the novel iron-regulated gene OsDPR is responsible for the nanism phenotype of transgenic seedlings because of the inhibition of plant cell proliferation.展开更多
基金Supported by the Scientific Research Project of Yunnan Agricultural University(A3007680)the Fund for Scientific Research of Department of Education+1 种基金Yunnan(2014y1902014y193)~~
文摘This study was conducted to establish a simple convenient method for calculating crop coefficient, and provide a certain basis for the research of the empirical formula for calculating crop coefficient with plant height which could be measured conveniently with regional differences, especially for the establishment of accurate irrigation schedule of potato in Yunnan. By the field experiment on potato under the condition of drip irrigation, it was found that the models of plant height with corrected FAO-56-recommended K and measured K were a quartic polynomial and a cubic polynomial, respectively, and the polynomial of potato plant height with measured crop coefficient was simpler with higher degree of fitting; and the differences between the period with the highest change rate of potato plant height and the periods with the greatest FAO-56-recommended K and measured K exhibited a differences of 3 d. In conclusion： In the future study of simple or empirical formula calculation of crop coefficient, plant height should be considered as a main dependent variable in that the calculation result would be closer to the measured crop coefficient with the problem of regional difference existing in the FAO method solved and the formula might be simpler; and the irrigation time of potato should be 3 d earlier than the irrigation time determined according to the corrected FAO-56-recommended crop coefficient, especially in the key water requirement stages of potato.
基金Supported by the Science and Technology Project of Food Production in Jiangxi Province(2006BAD02A04)~~
文摘[Objective] The aim was to carry out the genetic analysis on plant height of rice（Oryza sativa L.）cultivated in different seasons.[Method] Three rice parents with great difference in plant height including CB1（83.1 cm）,CB4（105.5 cm）and CB7（115.6 cm）were chosen to construct two parental combinations：CB1×CB4 and CB7×CB4,and the corresponding filial generations P1,F1,P2,B1,B2 and F2 were obtained.The 6 populations were planted in middle and late seasons respectively to measure their height traits.The Akaike＇s information criterion（AIC）of the mixed major gene and polygene model was used to indentify the existence of major genes affecting quantitative traits in B1,B2,F2 populations.When the major genes existed,the genetic effects of the major genes and polygenes and their genetic variance were estimated through segregation analysis.[Result] One additive major gene plus additive-dominance polygenes was the most fitted genetic model for the trait in all B1,B2,F2 populations in two planting seasons.The heritability values of the major genes varied from 38.63% to 78.53% and those of polygenes varied from 1.72% to 36.04%,and the total heritability values were 45.52-92.93%.The additive effect d value of the two genetic populations under two planting seasons was-4.56,-9.16,-7.19,and-9.38,respectively,as suggested that additive effect of the major genes would decrease the express of the plant height trait.[Conclusion] The heritability of plant height trait was affected by planting seasons and the combinations clearly as a whole.
基金Supported by the National Natural Science Foundation of China(31160254)~~
文摘[Objective] The research aimed to study the circadian rhythm of nitrate re- ductase activity （NRA） in plant. [Method] The wheat plants at heading stage were used as the materials for the measurement of dynamic changes of nitrate reductase activity （NRA） within 24 h under the conditions of constant high temperature. [Resulti The fluctuation of NRA in wheat changed greatly from 20：00 pm to 11：00 am. The enzyme activity remained constant, but at 14：00 the enzyme activity was the high- est, higher than all the other time points except the enzyme activity measured at11：00. The enzyme activity was the lowest of 17：00, which was lower than all the other time points except the enzyme activity measured at 2：00. [Conclusion] There were autonomous rhythm changes of NRA in wheat in a certain degree.
基金Supported by the National Natural Science Foundation of China(3077127231171483)+1 种基金the Priority Academic Program Development of Jiangsu Higher Education Institutions,Jiangsu Innovation Project for Agriculture Science and Technology [cx(11)2054 ]Jiangsu Agriculture Science and Technology Support Program(SBE2010307)
文摘[Objective] The study aimed to find a possible way to combat or alleviate the negative effects caused by high temperature and water deficit at the growth stage of peak boll-setting.[Method] With Bt transgenic cotton GK22 as the test cultivar,a potted experiment was carried out to investigate the effects of the regulation of external substances(the water solutions of pix,urea and their mixture) on the physiological parameters,insecticidal protein content,yield and yield component of cotton plants in artificial climate chambers treated with high temperature and water deficit.[Result] The application of external pix,urea or their mixture was effective in stabilizing the physiological parameters of cotton plants,insecticidal protein content,yield and yield components.Compared with the exclusive application of pix and urea,the mixture of pix and urea played the most effective role in stabilizing the content of chlorophyll,soluble sugar and insecticidal protein,alleviating the increase of the content of free amino acids and proline,and increasing boll number per plant,boll weight and seed cotton yield.[Conclusion] The water solutions of pix,urea or their mixtures can be used to combat or alleviate the stress of high temperature and water deficit if they are sprayed onto cotton plants prior to stress occurrence.
基金supported by the National Natural Science Foundation of China (Grant No. 30170552)the Academic and Technical Development Key Project Foundation of Beijing Education Community (Grant No. KZ200710028013)
文摘Preliminary microarray analysis of cDNA from rice roots revealed an up-regulated transcript that was highly expressed in a five-day iron deficiency treatment. The entire sequence of this gene was determined by bioinformatics analysis. There were no proteins with significant levels of similarity detected in public databases. This novel gene with unknown biological function was designated as OsDPR (dwarf phenotype-related gene). We constructed a stable plant expression vector pCAM- BIA1302-OsDPR::GFP and produced transgenic tobacco plants. The phenotypes suggested that OsDPR restrained the growth of transformed plants. To understand the mechanisms of this suppression effect, cell size and number were compared between transformants and wild-type plants. The cell proliferation rate was lower in OsDPR transgenic BY-2 cells than in wild-type cells, but OsDPR expression did not affect cell size. Moreover, the cell division-related gene CyclinD2.1, which is involved in plant growth, was down-regulated in transgenic tobacco plants. These findings suggested that the novel iron-regulated gene OsDPR is responsible for the nanism phenotype of transgenic seedlings because of the inhibition of plant cell proliferation.
