Triterpenoid saponins from Symplocos chinensis have been investigated by positive ion electrospray ionization multi-stage tandem mass spectrometry (ESI-MS^n) in a new linear ion trap mass spectrometer. It has been fou...Triterpenoid saponins from Symplocos chinensis have been investigated by positive ion electrospray ionization multi-stage tandem mass spectrometry (ESI-MS^n) in a new linear ion trap mass spectrometer. It has been found that the fragmentation patterns of these given saponins significantly vary in accordance with different derivatization in the glucuronyl residue of oligosaccharide chains. The product ions show that the carboxyl group and the free hydroxyl group at the C-3' position of the glucuronyl residue are the key sites for determining the fragmentation pathways of these given triterpenoid saponins.The described results in this paper confirm the mechanisms previously postulated for cross-ring cleavage process for the metal coordinated species. Furthermore, the experimental results can benefit the determination of the minute compounds of this type in crude plant extracts and give some aid for the studies of similar glycoconjugates.展开更多
目的研究木通科植物白木通藤茎的化学成分。方法通过反复硅胶柱色谱、ODS柱色谱和重结晶等方法进行分离纯化,根据化合物的理化性质和波谱数据鉴定结构。结果从白木通藤茎70%乙醇提取物中分离得到6个化合物。分别鉴定为2,α3,β23-trihyd...目的研究木通科植物白木通藤茎的化学成分。方法通过反复硅胶柱色谱、ODS柱色谱和重结晶等方法进行分离纯化,根据化合物的理化性质和波谱数据鉴定结构。结果从白木通藤茎70%乙醇提取物中分离得到6个化合物。分别鉴定为2,α3,β23-trihydroxyolean-12-en-28-oic ac idO-β-D-xylopyranosyl-(1→3)-O-α-L-rhamnopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester(I),2,α3,β23-trihydroxyurs-12-en-28-oic ac idO--βD-xylopyranosyl-(1→3)-O-α-L-rhamnopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester(II),2α,3,β23-trihydroxyolean-12-en-28-oic ac idO-α-L-rhamnopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosylester(III),2,α3,β23-trihydroxyurs-12-en-28-oic ac idO-α-L-rhamnopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester(IV),2,α3,β23-trihydroxyolean-12-en-28-oic ac idO--βD-glucopyranosyl ester(V),2,α3,β23-trihydroxyurs-12-en-28-oic ac idO-β-D-glucopyranosyl ester(V I)。结论化合物II为新化合物,命名为mutongsapon inC,其他均为首次从该植物藤茎中分离得到。展开更多
文摘Triterpenoid saponins from Symplocos chinensis have been investigated by positive ion electrospray ionization multi-stage tandem mass spectrometry (ESI-MS^n) in a new linear ion trap mass spectrometer. It has been found that the fragmentation patterns of these given saponins significantly vary in accordance with different derivatization in the glucuronyl residue of oligosaccharide chains. The product ions show that the carboxyl group and the free hydroxyl group at the C-3' position of the glucuronyl residue are the key sites for determining the fragmentation pathways of these given triterpenoid saponins.The described results in this paper confirm the mechanisms previously postulated for cross-ring cleavage process for the metal coordinated species. Furthermore, the experimental results can benefit the determination of the minute compounds of this type in crude plant extracts and give some aid for the studies of similar glycoconjugates.
文摘目的研究木通科植物白木通藤茎的化学成分。方法通过反复硅胶柱色谱、ODS柱色谱和重结晶等方法进行分离纯化,根据化合物的理化性质和波谱数据鉴定结构。结果从白木通藤茎70%乙醇提取物中分离得到6个化合物。分别鉴定为2,α3,β23-trihydroxyolean-12-en-28-oic ac idO-β-D-xylopyranosyl-(1→3)-O-α-L-rhamnopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester(I),2,α3,β23-trihydroxyurs-12-en-28-oic ac idO--βD-xylopyranosyl-(1→3)-O-α-L-rhamnopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester(II),2α,3,β23-trihydroxyolean-12-en-28-oic ac idO-α-L-rhamnopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosylester(III),2,α3,β23-trihydroxyurs-12-en-28-oic ac idO-α-L-rhamnopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester(IV),2,α3,β23-trihydroxyolean-12-en-28-oic ac idO--βD-glucopyranosyl ester(V),2,α3,β23-trihydroxyurs-12-en-28-oic ac idO-β-D-glucopyranosyl ester(V I)。结论化合物II为新化合物,命名为mutongsapon inC,其他均为首次从该植物藤茎中分离得到。
