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Agroinoculation as a Simple Way to Deliver a Tobacco Mosaic Virus- Based Expression Vector 被引量:7
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作者 贾洪革 庞永奇 方荣祥 《Acta Botanica Sinica》 CSCD 2003年第7期770-773,共4页
烟草花叶病毒(TMV)表达载体30B是一个目前广泛应用的植物病毒表达载体,但用其生产外源蛋白时,必须先将它体外转录成RNA,才能被用来接种宿主植物。由于RNA体外转录费用昂贵、操作复杂,因此限制了30B表达载体的进一步应用。针对这一不足,... 烟草花叶病毒(TMV)表达载体30B是一个目前广泛应用的植物病毒表达载体,但用其生产外源蛋白时,必须先将它体外转录成RNA,才能被用来接种宿主植物。由于RNA体外转录费用昂贵、操作复杂,因此限制了30B表达载体的进一步应用。针对这一不足,我们用农杆菌接种法(agroinoculation)接种该病毒载体,即将30B cDNA置于花椰菜花叶病毒(CaMV)的35S启动子和终止子之间,再将整个表达框架插入到农杆菌T-DNA的左边界和右边界之内,构建成质粒p35S-30B,将转入该质粒的农杆菌注射到植物的叶片中,30B cDNA随T-DNA进入植物细胞后,被转录成可自我复制的RNA形式,进而发生系统侵染。为了检测此接种方式的可行性,绿色荧光蛋白(GFP)报告基因被克隆到p35S-30B中,构建成p35S-30B∶∶GFP,用含有该质粒的农杆菌进行注射操作。证实该病毒载体可通过简便的农杆菌接种法侵染Nicotiana benthamiana,在被接种植物的系统叶中,GFP的表达量可占植物总可溶蛋白的5.2%。 展开更多
关键词 tobacco mosaic virus agroinoculation gene expression
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如果没有病毒世界会怎样
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作者 奇云 《海峡科技》 2003年第7期22-24,共3页
关键词 病毒 生命物质 生态平衡 生物进化 植物病毒 基因治疗 基因工程
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禾谷镰刀菌剪接体蛋白FgSnu114上的自发突变部分恢复Fgprp4的生长缺陷
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作者 赵晓斐 宋超妮 +1 位作者 曹心雨 金巧军 《菌物学报》 CAS CSCD 北大核心 2023年第8期1730-1743,共14页
由剪接体催化的前体mRNA剪接是一个重要的生物过程。Prp4作为剪接体组分中唯一的蛋白激酶,在剪接体的组装及激活过程中发挥关键作用。禾谷镰刀菌的Fgprp4突变体生长缺陷严重,易角变。本研究以251株Fgprp4突变体的角变子为着入点,鉴定了U... 由剪接体催化的前体mRNA剪接是一个重要的生物过程。Prp4作为剪接体组分中唯一的蛋白激酶,在剪接体的组装及激活过程中发挥关键作用。禾谷镰刀菌的Fgprp4突变体生长缺陷严重,易角变。本研究以251株Fgprp4突变体的角变子为着入点,鉴定了U5蛋白FgSnu114上的4个角突变位点。通过对角变子和模拟角变子(FgSNU114引入角突变并敲除FgPRP4的突变体)的表型比较验证了角突变D222N和ΔK695,明确FgPrp4和FgSnu114间的遗传互作。RNA-Seq分析发现角变子S172中约有23%的内含子具有剪接缺陷,解释了其有性生殖和侵染小麦的缺陷。进一步的磷酸化位点鉴定和模拟磷酸化实验证明FgSnu114的S451位点可能不是FgPrp4的磷酸化位点或关键磷酸化位点。本研究首次探讨了Prp4与Snu114之间的关系,有助于今后进一步揭示禾谷镰刀菌前体mRNA剪接的调控机制。 展开更多
关键词 Snu114 前体mRNA剪接 Prp4 角变子 植物侵染
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生长素合成途径的研究进展 被引量:40
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作者 王家利 刘冬成 +1 位作者 郭小丽 张爱民 《植物学报》 CAS CSCD 北大核心 2012年第3期292-301,共10页
生长素是一类含有一个不饱和芳香族环和一个乙酸侧链的内源激素,参与植物生长发育的许多过程。植物和一些侵染植物的病原微生物都可以通过改变生长素的合成来调节植株的生长。吲哚-3-乙酸(IAA)是天然植物生长素的主要活性成分。近年来,... 生长素是一类含有一个不饱和芳香族环和一个乙酸侧链的内源激素,参与植物生长发育的许多过程。植物和一些侵染植物的病原微生物都可以通过改变生长素的合成来调节植株的生长。吲哚-3-乙酸(IAA)是天然植物生长素的主要活性成分。近年来,随着IAA生物合成过程中一些关键调控基因的克隆和功能分析,人们对IAA的生物合成途径有了更加深入的认识。IAA的生物合成有依赖色氨酸和非依赖色氨酸两条途径。依据IAA合成的中间产物不同,依赖色氨酸的生物合成过程通常又划分成4条支路:吲哚乙醛肟途径、吲哚丙酮酸途径、色胺途径和吲哚乙酰胺途径。该文综述了近几年在IAA生物合成方面取得的新进展。 展开更多
关键词 摘要生长素是~类含有一个不饱和芳香族环和一个乙酸侧链的内源激素 参与植物生长发育的许多过程.植物和一些植物的病原微生物都可以通过改变生长素的合成来调节植株的生长.吲哚.3.乙酸0AA)是天然植物生长素的主要活性成分.近年来 随着IAA生物合成过程中一些关键调控基因的克隆和功能分析 人们对IAA的生物合成途径有了更加深入的认识.IAA的生物合成有依赖色氨酸和非依赖色氨酸两条途径.依据IAA合成的中间产物不同 依赖色氨酸的生物合成过程通常又划分成4条支路:吲哚乙醛肟途径 吲哚丙酮酸途径 色胺途径和吲哚乙酰胺途径.该文综述了近几年在IAA生物合成方面取得的新进展.
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Viral infection of tobacco plants improves performance of Bemisia tabaci but more so for an invasive than for an indigenous biotype of the whitefly 被引量:15
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作者 Jian LIU Meng LI +4 位作者 Jun-min LI Chang-jun HUANG Xue-ping ZHOU Fang-cheng XU Shu-sheng LIU 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2010年第1期30-40,共11页
The ecological effects of plant-virus-vector interactions on invasion of alien plant viral vectors have been rarely investigated. We examined the transmission of Tomato yellow leaf cur/China virus (TYLCCNV) by the i... The ecological effects of plant-virus-vector interactions on invasion of alien plant viral vectors have been rarely investigated. We examined the transmission of Tomato yellow leaf cur/China virus (TYLCCNV) by the invasive Q biotype and the indigenous ZHJ2 biotype of the whitefly Bemisia tabaci, a plant viral vector, as well as the influence of TYLCCNV-infection of plants on the performance of the two whitefly biotypes. Both whitefly biotypes were able to acquire viruses from infected plants and retained them in their bodies, but were unable to transmit them to either tobacco or tomato plants. However, when the Q biotype fed on tobacco plants infected with TYLCCNV, its fecundity and longevity were increased by 7- and 1-fold, respectively, compared to those of the Q biotype fed on uninfected tobacco plants. When the ZHJ2 biotype fed on virus-infected plants, its fecundity and longevity were increased by only 2- and 0.5-fold, respectively. These data show that the Q biotype acquired higher beneficial effects from TYLCCNV-infection of tobacco plants than the ZHJ2 biotype. Thus, the Q biotype whitefly may have advantages in its invasion and displacement of the indigenous ZHJ2 biotype. 展开更多
关键词 Bemisia tabaci BIOTYPES BEGOMOVIRUS Vector-virus interaction Biological invasion
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一例向日葵病毒病的检测与诊断
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作者 文朝慧 何苏琴 +1 位作者 王军平 庞博 《中国植保导刊》 北大核心 2012年第10期11-13,共3页
为诊断一例表现花叶、明脉和皱缩症状的向日葵病毒病病原,通过血清学及分子生物学方法对病样进行了检测。ELISA和RT-PCR的结果表明,病样受到TMV、WMV和LMV复合侵染。该结果为TMV、WMV和LMV在甘肃向日葵上发生侵染的首次报道。
关键词 向日葵:植物病毒病:复合
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Antioxidative Responses and Metal Accumulation in Invasive Plant Species Growing on Mine Tailings in Zanjan, Iran 被引量:1
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作者 M.M.A.BOOJAR Z.TAVAKKOLI 《Pedosphere》 SCIE CAS CSCD 2011年第6期802-812,共11页
Tailings of a Pb and Zn mine as a metal-contaminated area (Zone 1) with two pioneer plant species, Peganum harmala and Zygophyllum fabago, were investigated and compared with a non-contaminated area (Zone 2) in th... Tailings of a Pb and Zn mine as a metal-contaminated area (Zone 1) with two pioneer plant species, Peganum harmala and Zygophyllum fabago, were investigated and compared with a non-contaminated area (Zone 2) in the vicinity. Total concentrations of Pb, Zn, and Cu in the soil of Zone 1 were 1 416, 2 217, and 426 mg kg-1, respectively, and all exceeded their ranges in the normal soils. The soil pH was in the neutral range and most of the physical and chemical characteristics of the soils from both zones were almost similar. The species Z. fabago accumulated higher Cu and Zn in its aerial part and roots than the normal plants. On the other hand, their concentrations did not reach the criteria that the species could be considered as a metal hyperaccumulator. The species P. harmala did not absorb metals in its roots; accordingly, the accumulation factor values of these metals were lower than 1. The contents of chlorophyll, biomass, malondialdehyde, and dityrosine in these two species did not vary significantly between the two zones studied. In Zone 1, leaf vacuoles of Z. fabago stored 35.6% and 43.2% of the total leaf Cu and Zn, respectively. However, in this species, the levels of phytochelatins (PCs) and glutathione (GSH) and antioxidant enzyme activities were significantly higher in Zone 1 than in Zone 2. In conclusion, metal exclusion in P. harmala and metal accumulation in Z. fabago were the basic strategies in the two studied pioneer species growing on the metal-contaminated zone. In response to metal stress, elevation in antioxidant enzyme activities, increases in the PCs and GSH levels in the aerial parts, and metal storage within vacuoles counteracted each other in the invasion mechanism of Z. ]abago. 展开更多
关键词 accumulation factor antioxidant enzymes GLUTATHIONE metal stress PHYTOCHELATINS
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