This study was to research the cryopreservation effect of plant antifieeze proteins(AFPs) on day 7 porcine expanded and hatched blastocysts that were frozen in 1.5 M glycerol by conventional slow freezing method. The ...This study was to research the cryopreservation effect of plant antifieeze proteins(AFPs) on day 7 porcine expanded and hatched blastocysts that were frozen in 1.5 M glycerol by conventional slow freezing method. The developmental rates of porcine embryos frozen with 0.5 mg/ml AFPs in freeze medium and cultured for 12 and 24 hours in vitro are 25% and 0%respectively. With the concentration of AFPs increased to 5 mg/ml, the corresponding values became 80% and 40%. The hatched rates for porcine embryos frozen with 0.5 mg/ml and 5 mg/ml of AFPs and cultured for 24 hours in vitro are 0% and 20% respectively..The developmental and hatched rate of the contnrol are all 0%(0/4). These results indicate that the survival rates of porcine expanded and hatched blastocyst can be improved by supplementing freeze medium with plant AFPs.展开更多
矿山遗迹地的生态修复是促进人与自然和谐共生、实现绿色低碳发展的重要举措。干旱区矿山遗迹地立地条件差,植被覆盖率低,采矿活动造成的土壤侵蚀强烈,确定科学合理的恢复模式是生态修复成功与否的关键。以宁夏中卫市的典型石灰岩矿山...矿山遗迹地的生态修复是促进人与自然和谐共生、实现绿色低碳发展的重要举措。干旱区矿山遗迹地立地条件差,植被覆盖率低,采矿活动造成的土壤侵蚀强烈,确定科学合理的恢复模式是生态修复成功与否的关键。以宁夏中卫市的典型石灰岩矿山遗迹地为研究对象,通过对比7种生态修复植物在不同土壤基质重构措施下的土壤温、湿度和有机质,以及植物的存活率和新生枝相对生长速率(Relative growth rate of new shoots,RGRs)等指标,筛选出适宜干旱区石灰岩矿山遗迹地生态修复的最优模式,构建了土壤改良-植被重建联合的生态恢复方案。结果表明:(1)土壤基质重构措施是石灰岩矿山生态修复的前提条件,有效地改善了植物定植环境,不同土壤基质重构模式的植物存活率表现为:土+有机肥>砂+土+有机肥>砂+土+复合肥;(2)土壤有机质的范围为0.56~2.84 g·kg^(-1),植物种和土壤基质及二者的交互作用对土壤有机质含量具有极显著影响(P<0.001);(3)RGRs的范围为1.51~1.87 mm·cm^(-1)·month^(-1),不同植物种的RGRs存在显著性差异;(4)通过系统聚类分析得到本研究区最适宜的生态修复模式为砂+土+有机肥(沙拐枣或短穗柽柳)。本研究从土壤改良-植被重建角度提出的联合生态修复方案可为干旱区矿山生态修复提供理论依据和实践参考。展开更多
文摘This study was to research the cryopreservation effect of plant antifieeze proteins(AFPs) on day 7 porcine expanded and hatched blastocysts that were frozen in 1.5 M glycerol by conventional slow freezing method. The developmental rates of porcine embryos frozen with 0.5 mg/ml AFPs in freeze medium and cultured for 12 and 24 hours in vitro are 25% and 0%respectively. With the concentration of AFPs increased to 5 mg/ml, the corresponding values became 80% and 40%. The hatched rates for porcine embryos frozen with 0.5 mg/ml and 5 mg/ml of AFPs and cultured for 24 hours in vitro are 0% and 20% respectively..The developmental and hatched rate of the contnrol are all 0%(0/4). These results indicate that the survival rates of porcine expanded and hatched blastocyst can be improved by supplementing freeze medium with plant AFPs.
文摘矿山遗迹地的生态修复是促进人与自然和谐共生、实现绿色低碳发展的重要举措。干旱区矿山遗迹地立地条件差,植被覆盖率低,采矿活动造成的土壤侵蚀强烈,确定科学合理的恢复模式是生态修复成功与否的关键。以宁夏中卫市的典型石灰岩矿山遗迹地为研究对象,通过对比7种生态修复植物在不同土壤基质重构措施下的土壤温、湿度和有机质,以及植物的存活率和新生枝相对生长速率(Relative growth rate of new shoots,RGRs)等指标,筛选出适宜干旱区石灰岩矿山遗迹地生态修复的最优模式,构建了土壤改良-植被重建联合的生态恢复方案。结果表明:(1)土壤基质重构措施是石灰岩矿山生态修复的前提条件,有效地改善了植物定植环境,不同土壤基质重构模式的植物存活率表现为:土+有机肥>砂+土+有机肥>砂+土+复合肥;(2)土壤有机质的范围为0.56~2.84 g·kg^(-1),植物种和土壤基质及二者的交互作用对土壤有机质含量具有极显著影响(P<0.001);(3)RGRs的范围为1.51~1.87 mm·cm^(-1)·month^(-1),不同植物种的RGRs存在显著性差异;(4)通过系统聚类分析得到本研究区最适宜的生态修复模式为砂+土+有机肥(沙拐枣或短穗柽柳)。本研究从土壤改良-植被重建角度提出的联合生态修复方案可为干旱区矿山生态修复提供理论依据和实践参考。
