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植物染色体显微切割技术的研究现状与展望 被引量:9
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作者 马有志 徐琼芳 辛志勇 《遗传》 CAS CSCD 北大核心 1997年第2期45-47,共3页
植物染色体显微切割技术的研究现状与展望马有志徐琼芳辛志勇(中国农业科学院作物育种栽培研究所,北京100081)TheAdvancesoftheTechniqueofPlantChromosomeMicrodisect... 植物染色体显微切割技术的研究现状与展望马有志徐琼芳辛志勇(中国农业科学院作物育种栽培研究所,北京100081)TheAdvancesoftheTechniqueofPlantChromosomeMicrodisectionMaYouzhiXuQion... 展开更多
关键词 植物学技术 染色体 显微切割
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增强化学发光基因检测技术 被引量:1
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作者 种康 黄华棕 +1 位作者 桂进 谭克辉 《植物生理学通讯》 CSCD 1993年第5期368-370,共3页
增强化学发光基因检测技术(enhanced chemiluminescence,简称ECL)是目前最灵敏的非放射性核酸检测方法之一。其灵敏度可以满足多数杂交技术的需要(达pg水平),并且具有快速显示杂交结果(曝光1~5min)和对人无伤害、对环境不会造成污染等... 增强化学发光基因检测技术(enhanced chemiluminescence,简称ECL)是目前最灵敏的非放射性核酸检测方法之一。其灵敏度可以满足多数杂交技术的需要(达pg水平),并且具有快速显示杂交结果(曝光1~5min)和对人无伤害、对环境不会造成污染等独特优点。在分子生物学研究中有广泛的用途。根据我们应用ECL基因检测系统(amersham)情况,将这一技术介绍如下。 ECL检测系统的工作原理是在氧化剂存在时。 展开更多
关键词 增强化学发光 基因检测 植物学技术
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植物组织的显微摄影和图版处理的一些问题 被引量:1
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作者 胡适宜 《植物学通报》 CSCD 1990年第3期58-62,共5页
对植物细胞和组织结构进行研究时,经常利用显微照相记录下观察到的结果。在生物学学术论文中也常借助显微照片再现显微镜下切片标本的形象,这可以省去许多文字的描述,而且对问题的阐述令人信服。图片不理想会使文章大为逊色,甚至影响到... 对植物细胞和组织结构进行研究时,经常利用显微照相记录下观察到的结果。在生物学学术论文中也常借助显微照片再现显微镜下切片标本的形象,这可以省去许多文字的描述,而且对问题的阐述令人信服。图片不理想会使文章大为逊色,甚至影响到对所得出的结论发生怀疑。可以认为在形态结构性质的研究中,由显微照片组成的图版是论文的一个很重要的组成部分。显微照相术已有上百年的历史,这一技术的原理和操作程序国内外都有专门的著作,一些生物学实验技术的教本中也有论述。国内出版的《生物科学摄影基础》。 展开更多
关键词 植物学技术 显微摄影 图版处理
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脂肪酸活力的比色测定法 被引量:3
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作者 林榕辉 朱智伟 《植物生理学通讯》 CSCD 1992年第5期376-378,共3页
脂肪酶(E.C.3.1.1.3)能将脂肪(甘油三酰)水解为甘油及游离脂肪酸。由于它是脂肪分解代谢中第一个参与反应的酶,所以一般认为它对脂肪的转化速率起着调控的作用。油料种子在萌芽时。
关键词 脂肪酶 活力 比色法 植物学技术
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一种快速叶表皮离析法 被引量:19
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作者 温洁 《植物学通报》 CSCD 1995年第A01期77-77,72,共2页
一种快速叶表皮离析法温洁(中国科学院植物研究所系统与进化植物学开放实验室,北京100093)ARAPIDMATHODOFISOLATINGANDCLEARINGORGANSONFOLIAREPIDERMIS¥WenJ... 一种快速叶表皮离析法温洁(中国科学院植物研究所系统与进化植物学开放实验室,北京100093)ARAPIDMATHODOFISOLATINGANDCLEARINGORGANSONFOLIAREPIDERMIS¥WenJie(LaboratoryofSy... 展开更多
关键词 植物学技术 叶表皮离析法 实验技术
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用离体叶细胞观察质壁分离和质壁分离复原
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作者 何生根 梁佳勇 《植物生理学通讯》 CSCD 1995年第4期292-292,共1页
关键词 实验技术 离体叶细胞 质壁分离 复原 植物学技术
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爱氏苏木精整体染色法在树脂薄切片中的应用 被引量:3
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作者 陈超 王桂兰 《植物学通报》 CSCD 1992年第4期53-54,共2页
在组织和细胞学研究中,整体染色法是一项很有用的技术。树脂薄切片技术是近20年来发展起来的一门新技术,它通常是先切后染,这样前后几次染色很难均一。对于需要长时间染色的材料就更显麻烦。另外,常用于薄切片中的染色剂甲苯胺蓝—O、... 在组织和细胞学研究中,整体染色法是一项很有用的技术。树脂薄切片技术是近20年来发展起来的一门新技术,它通常是先切后染,这样前后几次染色很难均一。对于需要长时间染色的材料就更显麻烦。另外,常用于薄切片中的染色剂甲苯胺蓝—O、亚甲基蓝和大青Ⅱ在用树胶封片制成永久切片后,颜色会逐渐退去,不能长期保存。为了解决这些问题,我们在掌叶大黄的胚胎学研究中,以掌叶大黄(Rheum palmatum)为材料, 展开更多
关键词 植物学技术 树脂薄切片 整体染色法 爱氏苏木精
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Comparison on Methods.for Extracting DNA from Pteridophyta 被引量:4
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作者 沈洁 罗安才 《Agricultural Science & Technology》 CAS 2009年第6期26-28,共3页
[ Objective] The aim was to study method for extracting DNA from pteridophyta and provide basis for further study on genetic diversity and taxonomy. [ Method] By changing the dosage of reagent and operating method, CT... [ Objective] The aim was to study method for extracting DNA from pteridophyta and provide basis for further study on genetic diversity and taxonomy. [ Method] By changing the dosage of reagent and operating method, CTAB method for extracting DNA was improved. [ Result] The results showed that the improved CTAB method could extract high-quality DNA from pteridophyta. [ Conclusion] The study improved method for extracting DNA from pteridophyta. 展开更多
关键词 Pteddophyta DNA extraction CTAB method
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Advances in Plant Science and Its Prospects
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作者 许智宏 《Agricultural Science & Technology》 CAS 2011年第1期1-7,10,共8页
Focusing on the global food security and analyzing the challenges now confronting human being of global climate change,sustainable development of agriculture,energy and environment,plant scientists should take the soc... Focusing on the global food security and analyzing the challenges now confronting human being of global climate change,sustainable development of agriculture,energy and environment,plant scientists should take the social responsibility and play the role in solving the global problems like food security from the developmental status and potential of plant science and technology. Moreover,by working with agronomists, ecologists and environmental experts,plant scientists do provide novel ideas and technologies for developing low carbon agriculture,for breeding new crop varieties with high yield and quality and stress resistance,for developing new type of energy plants,for supplying healthy foods and drugs,reasonably using land and water resources,and for ameliorating environment,making better contribution to the sustainable development of the globe and human being ourselves. 展开更多
关键词 Plant science Food security BIOTECHNOLOGY TRANSGENE Medicinal plant Energy plant
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Current progress and future prospects in phylofloristics 被引量:8
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作者 Rong Li Lishen Qian Hang Sun 《Plant Diversity》 SCIE CAS CSCD 2018年第4期141-146,共6页
The species composition of regional plant assemblages can in large part be explained by a long history of biogeographical and evolutionary events. Traditional attempts of floristic studies typically focus on the analy... The species composition of regional plant assemblages can in large part be explained by a long history of biogeographical and evolutionary events. Traditional attempts of floristic studies typically focus on the analyses of taxonomic composition, often ignoring the rich context that evolutionary history can provide. In 2014, Swenson and Umafia introduced the term 'phylofloristics' to define a phylogenetically enabled analysis of the species composition of regional floras. Integrating phylogenetic information into tradi- tional floristic analysis can provide a promising way to explore the ecological, biogeographic, and evolutionary processes that drive plant assemblies at multiple spatial scales. In this review, we sum- marize the current progress on the phylogenetic structure, spatial phylogenetic pattern, origin and diversification, phylogenetic regionalization of fioristic assemblages, and application of phylogenetic information in biodiversity conservation. These summaries highlight the importance of incorporating phylogenetic information to improve our understanding of floristic assembly from an evolutionary perspective. The review ends with a brief outlook on future challenges for phylofloristic studies, including generating a highly resolved species-level phylogenetic tree, compiling detailed and refined information regarding the geographic distribution of all plant life, extracting trait information from publications and herbarium specimens, and developing technological and methodological approaches for big data analysis. 展开更多
关键词 Big data BIOGEOGRAPHY ECOLOGY Evolution FLORA Mega-phylogeny
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Suitable Factors of Saussurea involucrata and Standardized Planting Technology 被引量:2
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作者 Nurbolat Yanrui CHEN +3 位作者 Min LIU Hui WANG Huixia NING Xiaojun WANG 《Agricultural Science & Technology》 CAS 2012年第5期990-992,共3页
Researches on biological characters, ecological functions and standardized planting technology of Saussurea in Xinjiang were conducted for the extension of the planting technology in Xinjiang, development of Chinese h... Researches on biological characters, ecological functions and standardized planting technology of Saussurea in Xinjiang were conducted for the extension of the planting technology in Xinjiang, development of Chinese herbal medicine and ecological restoration in high mountains, forming a system of standardized planting technology for Saussurea, including seedling culture, transplanting, regulation in field and prevention against diseases 展开更多
关键词 Saussurea involucrata HABITAT Standardized planting Pest prevention
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Photochemical Method to Evaluate Oxidation Levels in Heat-Treated Vegetable Oil: Comparison with Peroxide-Value and Acid-Value
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作者 Hiromi Kameya Mitsuko Ukai 《Journal of Food Science and Engineering》 2013年第6期299-308,共10页
To determine the degree of oxidation in vegetable oil, a photochemical method using the electron spin resonance (ESR) spin trapping technique was developed, and the results were compared with those obtained using co... To determine the degree of oxidation in vegetable oil, a photochemical method using the electron spin resonance (ESR) spin trapping technique was developed, and the results were compared with those obtained using conventional peroxide-value (PV) and acid-value (AV) methods. Vegetable oil heat-treated were subjected to short UV illumination, and the produced alkyl-oxyl radicals were identified and quantified using the ESR spin trapping technique. ESR signal intensity was used as an indicator of oil oxidation, which monotonically increased as a function of the heat-treatment time. Commercially, available oils were selected and analyzed. The results were compared with those obtained using the PV and AV methods. The present method displayed some analogies with the PV method at least in the early stage of heating. Overall, the present method had highly sensitive and capable of detecting early-stage oxidation in vegetable oil. 展开更多
关键词 ESR spin trapping plant oil peroxy radical UV-illumination.
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