Immature embryos, mature embryos and embryogenic calli of 6 rice (Oryza sativa L.) materials were transformed with particle bombardment. The plasmids pSSVstl and pVE5+ were used, both containing the phytoalexin gene f...Immature embryos, mature embryos and embryogenic calli of 6 rice (Oryza sativa L.) materials were transformed with particle bombardment. The plasmids pSSVstl and pVE5+ were used, both containing the phytoalexin gene from grapevine coding for stilbene synthase, but driven by 35S and its own promoter respectively. Through resistance selection for G418 (100 to 150 mg/L) or hygromycin (50 mg/L), 54 independent transgenic plants were isolated and further assessed by PCR, Southern blot and Dot blot analyses. The transgenic plants and their progenies were tested for resistance to blast ( Pyricularia oryzae ) and bacterial blight of rice ( Xanthomonas oryzae ). Preliminary results indicated that the stilbene synthase gene could enhance the resistance of transgenic plants and their progenies to both pathogens.展开更多
Sesquiterpenoids are a class of 15-carbon secondary metabolites that play diverse roles in plant adaptation to environment. Cotton plants accumulate a large amount of sesquiterpene aldehydes （including gossypol） as ...Sesquiterpenoids are a class of 15-carbon secondary metabolites that play diverse roles in plant adaptation to environment. Cotton plants accumulate a large amount of sesquiterpene aldehydes （including gossypol） as phytoalexins against pathogens and herbivores. They are stored in pigment glands of aerial organs and in epidermal layers of roots. Several enzymes of goss- ypol biosynthesis pathway have been characterized, including 3-hydroxy-3-methylglutaryl coenzyme A reductase （HMGR） and farnesyl diphosphate synthase （FPS） that catalyze the formation of the precursor farnesyl diphosphate （FPP）, （＋）-6-cadinene synthase （CDN） which is the first enzyme committed to gossypol biosynthesis, and the downstream enzymes of CYP706B 1 and methyltransferase. Expressions of these genes are tightly regulated during cotton plants development and in- duced by jasmonate and fungi elicitors. The transcription factor GaWRKY1 has been shown to be involved in gossypol path- way regulation. Recent development of new genomic platforms and methods and releases of diploid and tetraploid cotton ge- nome sequences will greatly facilitate the elucidation of gossypol biosynthetic pathway and its regulation.展开更多
文摘Immature embryos, mature embryos and embryogenic calli of 6 rice (Oryza sativa L.) materials were transformed with particle bombardment. The plasmids pSSVstl and pVE5+ were used, both containing the phytoalexin gene from grapevine coding for stilbene synthase, but driven by 35S and its own promoter respectively. Through resistance selection for G418 (100 to 150 mg/L) or hygromycin (50 mg/L), 54 independent transgenic plants were isolated and further assessed by PCR, Southern blot and Dot blot analyses. The transgenic plants and their progenies were tested for resistance to blast ( Pyricularia oryzae ) and bacterial blight of rice ( Xanthomonas oryzae ). Preliminary results indicated that the stilbene synthase gene could enhance the resistance of transgenic plants and their progenies to both pathogens.
基金the State Key Basic Research Program of China (2013CB127000)the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB11030300)+1 种基金the National Natural Science Foundation of China (31300255)the Special Fund for Shanghai Landscaping Administration Bureau Program (G142425, F112418).
文摘Sesquiterpenoids are a class of 15-carbon secondary metabolites that play diverse roles in plant adaptation to environment. Cotton plants accumulate a large amount of sesquiterpene aldehydes （including gossypol） as phytoalexins against pathogens and herbivores. They are stored in pigment glands of aerial organs and in epidermal layers of roots. Several enzymes of goss- ypol biosynthesis pathway have been characterized, including 3-hydroxy-3-methylglutaryl coenzyme A reductase （HMGR） and farnesyl diphosphate synthase （FPS） that catalyze the formation of the precursor farnesyl diphosphate （FPP）, （＋）-6-cadinene synthase （CDN） which is the first enzyme committed to gossypol biosynthesis, and the downstream enzymes of CYP706B 1 and methyltransferase. Expressions of these genes are tightly regulated during cotton plants development and in- duced by jasmonate and fungi elicitors. The transcription factor GaWRKY1 has been shown to be involved in gossypol path- way regulation. Recent development of new genomic platforms and methods and releases of diploid and tetraploid cotton ge- nome sequences will greatly facilitate the elucidation of gossypol biosynthetic pathway and its regulation.
