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植物枯萎病拮抗细菌KL-1菌株的分离及鉴定 被引量:3
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作者 戴君勇 贲爱玲 +2 位作者 吴向华 吴敏敏 陈玲 《安徽农业科学》 CAS 北大核心 2010年第34期19404-19407,共4页
[目的]筛选、鉴定植物枯萎病菌拮抗细菌,为植物枯萎病生防制剂的研究奠定基础。[方法]采用对峙培养法筛选对植物枯萎病菌具有良好抑制作用的生防细菌,通过Biolog细菌自动鉴定系统和16SrDNA序列分析法鉴定其分类地位,并研究该菌株对棉花... [目的]筛选、鉴定植物枯萎病菌拮抗细菌,为植物枯萎病生防制剂的研究奠定基础。[方法]采用对峙培养法筛选对植物枯萎病菌具有良好抑制作用的生防细菌,通过Biolog细菌自动鉴定系统和16SrDNA序列分析法鉴定其分类地位,并研究该菌株对棉花和小鼠的安全性。[结果]从棉花根际土壤中分离出的12株细菌中,有5株对多隔镰刀菌、香石竹尖孢镰刀菌、棉花枯萎病菌具有拮抗活性;其中,KL-1菌株对3株目标病原菌的抑菌活性分别达69.09%、80.78%、78.89%。Biolog细菌自动鉴定系统和16SrDNA序列分析法鉴定结果表明,KL-1菌株为解淀粉芽孢杆菌;生物安全性初步测定结果表明,KL-1菌株对棉花和小鼠安全无毒。[结论]解淀粉芽孢杆菌KL-1菌株具有对多种植物枯萎病菌的抑菌活性,且对棉花和小鼠安全无毒,是一株具有研究和开发潜能的生防菌株。 展开更多
关键词 植物枯萎病 拮抗细菌 解淀粉芽孢杆菌 鉴定
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植物镰刀菌枯萎病防治的研究进展 被引量:8
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作者 施祖国 《现代农业科技》 2016年第20期102-103,共2页
根据近年来国内外对镰刀菌枯萎病研究成果,综述镰刀菌枯萎病化学防治和生物防治2个方面的研究进展,并对今后的研究方向进行了展望。
关键词 植物镰刀菌枯萎病 化学防治 农业防治 研究进展
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致病性尖孢镰刀菌毒力因子的研究进展 被引量:12
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作者 李英 杜春梅 《中国农学通报》 2021年第12期92-97,共6页
致病性尖孢镰刀菌(Fusarium oxysporum)侵染植物引发的植物枯萎病是世界范围内广泛存在的毁灭性土传真菌病害,目前该菌已被列为世界上第五大植物病原真菌。由于病原菌通过土壤传播,其致病机理尚未完全明确,而充分了解尖孢镰刀菌的致病... 致病性尖孢镰刀菌(Fusarium oxysporum)侵染植物引发的植物枯萎病是世界范围内广泛存在的毁灭性土传真菌病害,目前该菌已被列为世界上第五大植物病原真菌。由于病原菌通过土壤传播,其致病机理尚未完全明确,而充分了解尖孢镰刀菌的致病机制是对抗这种疾病的先决条件。为了更好的研究和实施有效控制策略从而限制宿主植物感染,本文归纳了尖孢镰刀菌的致病机理,总结了毒力因子中的细胞壁降解酶、毒素、信号传导途径(MAPK通路)对致病进程的影响,分析了毒力基因及转录因子在感染过程中的调控机理。以期能为防控尖孢镰刀菌引起的枯萎病提供作用靶点和理论依据。 展开更多
关键词 尖孢镰刀菌 植物枯萎病 土传真菌病害 植物病原真菌 细胞壁降解酶 毒素 毒力基因 转录因子
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Inhibitive Activity of the Extracts of Plants in Artemisia against Fusarium oxysporum f. sp. vasinfectum and Fusarium momiliforme 被引量:2
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作者 郭艳 潘月敏 +3 位作者 高智谋 王坤 彭梅 曹舜 《Agricultural Science & Technology》 CAS 2010年第2期128-131,154,共5页
[Objective]The aim was to provide theoretical basis for the control of cotton Fusarium wilt and cotton boll rot disease.[Method]The inhibitory activity of the extracts from three species of plants(Artemisia annual L.,... [Objective]The aim was to provide theoretical basis for the control of cotton Fusarium wilt and cotton boll rot disease.[Method]The inhibitory activity of the extracts from three species of plants(Artemisia annual L.,Artemisia capillaris Thunb.,Artemisia argyi Levl.et Vant)against Fusarium oxysporum f.sp.vasinfectum and Fusarium moniliforme were studied under the condition of laboratory.[Result]The extracts of all the three plants in Artemisia showed strong antifungal activity against the tested pathogenic ... 展开更多
关键词 Artemisia extracts Antifungal activities Fusarium oxysporum f.sp.vasinfectum Fusarium momiliforme
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Occurrence and Effects of Pineapple Mealybug Wilt Disease in Central Uganda
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作者 Bosco Bua Jeninah Karungi Geoffrey Kawube 《Journal of Agricultural Science and Technology(A)》 2013年第5期410-416,共7页
Pineapple mealybug wilt disease (PMWD) is one of the latest outbreaks of diseases attacking pineapple in Uganda. However, its occurrence and effects have not been documented and quantified, yet the disease poses a s... Pineapple mealybug wilt disease (PMWD) is one of the latest outbreaks of diseases attacking pineapple in Uganda. However, its occurrence and effects have not been documented and quantified, yet the disease poses a serious threat to the pineapple industry. Therefore, the objective of this study was to assess the occurrence and effects of PMWD on pineapple in central Uganda. Semi-structured questionnaire was used to solicit information from 82 respondents consisting of farmers, opinion leaders, key informants, political and technical leadership during May 2011. PMWD was observed in all the fields surveyed but with varying incidences and severities. In addition, PMWD was more common during the dry seasons than the rainy seasons where higher incidences were associated with high mealybug populations. PMWD manifested as a syndrome characterized by yellowing of leaves, stunting, wilting and rotting of roots. The effects of PMWD were variable but yield reductions and low plant populations were widely reported. Although, the occurrence of PMWD was reported to the different level of authority in the districts, very little was done to curb its spread. 展开更多
关键词 Control EFFECTS PERCEPTIONS pineapple mealybug wilt disease occurrence.
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Advances on the Molecular Mechanism of the Interaction between Ralstonia solanacearum and Hosts
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作者 Dousheng WU Gaofei JIANG +2 位作者 Yanmei YU Ying LIU Wei DING 《Agricultural Science & Technology》 CAS 2013年第12期1698-1701,1718,共5页
Ralstonia solanacearum is an important model phytopathogenic bacterium that causes bacterial wilt disease on many plant species and leads to serious economic losses. The interactions between R. solanacearum and host p... Ralstonia solanacearum is an important model phytopathogenic bacterium that causes bacterial wilt disease on many plant species and leads to serious economic losses. The interactions between R. solanacearum and host plants have become a model system for the study of plants and pathogens interactions. This paper reviews the advances on the molecular mechanisms between R. solanacearum and hosts interaction including the formation of plant innate immunity, the suppression of plant innate immunity by this pathogen and the activation of effector-triggered immunity. Furthermore, we made a prospect on how to utilize the interaction mechanism between R. solanacearum and hosts to control the disease. 展开更多
关键词 Ralstonia solanacearum Host plants Interaction Plant immunity
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尖孢镰刀菌致病相关因子及其分子生物学研究进展 被引量:24
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作者 叶旭红 林先贵 王一明 《应用与环境生物学报》 CAS CSCD 北大核心 2011年第5期759-762,共4页
由致病性尖孢镰刀菌(Fusarium oxysporum)侵染引起的植物枯萎病是一种世界性的土传真菌病害,对农业生产造成了巨大经济损失.研究表明,尖孢镰刀菌的致病与多种致病因子相关,包括信号传导系统(环腺苷酸单磷酸-蛋白激酶A和促分裂素原活化... 由致病性尖孢镰刀菌(Fusarium oxysporum)侵染引起的植物枯萎病是一种世界性的土传真菌病害,对农业生产造成了巨大经济损失.研究表明,尖孢镰刀菌的致病与多种致病因子相关,包括信号传导系统(环腺苷酸单磷酸-蛋白激酶A和促分裂素原活化蛋白激酶信号途径)、细胞壁降解酶(木聚糖酶、果胶酶、果胶裂解酶、细胞毒素、纤维素酶、半纤维素酶和木质素降解酶等)、克服植物防御响应系统(降解抗真菌化合物和破坏植物细胞壁的化学修饰)以及其它(细胞毒素和过氧化物酶)等.此外对今后的研究工作提出展望. 展开更多
关键词 尖孢镰刀菌 致病因子 分子生物学 植物枯萎病 信号传导 细胞壁降解酶 防御响应
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