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植物DNA条形码技术的发展及应用 被引量:44
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作者 刘宇婧 刘越 +1 位作者 黄耀江 龙春林 《植物资源与环境学报》 CAS CSCD 2011年第1期74-82,93,共10页
在对DNA条形码技术的发展过程进行归纳分析的基础上,对植物DNA条形码技术的研究进展、工作流程及分析方法、影响其鉴定准确性的因素及其在植物分类学研究中的应用现状及存在的争议进行了综合分析和阐述,并展望了植物DNA条形码技术的发... 在对DNA条形码技术的发展过程进行归纳分析的基础上,对植物DNA条形码技术的研究进展、工作流程及分析方法、影响其鉴定准确性的因素及其在植物分类学研究中的应用现状及存在的争议进行了综合分析和阐述,并展望了植物DNA条形码技术的发展趋势及应用前景。通过具体实例说明将植物DNA条形码技术与传统植物学知识相结合可作为民族植物学的研究手段之一。认为:目前常用的植物DNA条形码主要有单一片段和多片段组合2种方式,这2种方式各有优缺点;常用的DNA序列有matK、trnH-psbA、rbcL和ITS等,但均有一定的局限性;针对不同的使用目的,应选择不同的植物DNA条形码标准;影响植物DNA条形码鉴定准确性的因素包括物种的类型和数量、系统树构建方法、杂交/基因渗入、物种起源时间的差异、分子进化速率差异等;当前植物DNA条形码研究工作的重点是选择合适的DNA片段并对其进行评价。 展开更多
关键词 DNA条形码 发展进程 分类学 植物物种鉴定 民族植物
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DNA宏条形码技术在动植物法医鉴定中的应用进展 被引量:6
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作者 李磊 蒋敬 陈云霞 《南京林业大学学报(自然科学版)》 CAS CSCD 北大核心 2021年第1期235-241,共7页
DNA宏条形码技术是随着2代高通量测序技术的发展而出现的一种物种鉴别技术。该技术结合了DNA条形码技术和高通量测序技术的优点,可以对混合样本中的多物种来源进行同步鉴定。本研究介绍DNA宏条形码技术的含义以及目前的应用需求,分析DN... DNA宏条形码技术是随着2代高通量测序技术的发展而出现的一种物种鉴别技术。该技术结合了DNA条形码技术和高通量测序技术的优点,可以对混合样本中的多物种来源进行同步鉴定。本研究介绍DNA宏条形码技术的含义以及目前的应用需求,分析DNA宏条形码技术在食品、药品监管、打击野生动植物犯罪、刑事案件侦查等司法实践中的应用现状,总结和讨论DNA宏条形码技术应用于动植物法医鉴定领域所面临的问题与挑战,对该技术的发展及应用前景进行展望。目前DNA宏条形码技术已被应用于生命科学的多个领域,但在动植物法医鉴定中的应用仍在起步阶段,虽然现在还面临着许多挑战,但随着测序技术与生物技术的发展,DNA宏条形码技术一定会在动植物法医鉴定领域实现常规化应用,为野生动植物相关犯罪活动的打击、食品药品安全性与合法性的监管提供技术支持。 展开更多
关键词 DNA条形码 DNA宏条形码 高通量测序 植物物种鉴定
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Preliminary Studies and Antimicrobial Evaluation of the Aerial Parts of Genista numidica ssp. numidica 被引量:1
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作者 Oumessaad Toubal Abdelghani Djahoudi Amel Bouzabata 《Journal of Life Sciences》 2011年第11期954-959,共6页
Genista numidica Spach ssp numidica (Spach) Batt. is a northeast Algerian endemic plant; the aim of this work is about the phytochemical screening, the microscopic analysis and the antimicrobial activity of the aeri... Genista numidica Spach ssp numidica (Spach) Batt. is a northeast Algerian endemic plant; the aim of this work is about the phytochemical screening, the microscopic analysis and the antimicrobial activity of the aerial parts of this species (stems, flowers, seeds and pods); there is a wealth in polyphenolic compounds: flavonoids (method of Charaux, Paris, 1964) and saponins (method of Braune et al, 1969), tannins, terpenes, sterols and fatty acids. The microscopic analysis doesn't allow a differential identification of the species. The results of aromatogram by the incorporation method of Muller-Hinton on solid medium, showed a significant antimicrobial activity (method Duraffourd,1987) of the extracts, infusion, and the active substances towards bacterial strains, essentially those recognized as antibiotic resistant (Staphylococcus, Acinetobacter, Pseudomonas, Enterobacter, Citrobacter); the results obtained indicate that the most efficient antibacterial activity was obtained with the pod infusion, the seed extract and the flavonoids. The raw extract has an important antifungal activity on Candida albicans. This could open opportunities for using this species in the treatment of urinary, respiratory, intestinals and cutaneous infections, and as a disinfecting additive on nosocomial area. 展开更多
关键词 Genista numidica ssp. numidica phytochemical screening fatty acids antimicrobial activity DISINFECTION
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UAVs as remote sensing platforms in plant ecology:review of applications and challenges 被引量:2
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作者 Zhongyu Sun Xiaonian Wang +3 位作者 Zhihui Wang Long Yang Yichun Xie Yuhui Huang 《Journal of Plant Ecology》 SCIE CSCD 2021年第6期1003-1023,共21页
Aims Unmanned aerial vehicles(UAVs),i.e.drones,have recently emerged as cost-effective and flexible tools for acquiring remote sensing data with fine spatial and temporal resolution.It provides a new method and opport... Aims Unmanned aerial vehicles(UAVs),i.e.drones,have recently emerged as cost-effective and flexible tools for acquiring remote sensing data with fine spatial and temporal resolution.It provides a new method and opportunity for plant ecologists to study issues from individual to regional scales.However,as a new method,UAVs remote sensing applications in plant ecology are still challenged.The needs of plant ecology research and the application development of UAVs remote sensing should be better integrated.Methods This report provides a comprehensive review of UAV-based remote sensing applications in plant ecology to synthesize prospects of applying drones to advance plant ecology research.Important Findings Of the 400 references,59%were published in remote sensing journals rather than in plant ecology journals,reflecting a substantial gap between the interests of remote sensing experts and plant ecologists.Most of the studies focused on UAV remote sensing’s technical aspects,such as data processing and remote sensing inversion,with little attention on answering ecological questions.There were 61%of studies involved community-scale research.RGB and multispectral cameras were the most used sensors(75%).More ecologically meaningful parameters can be extracted from UAV data to better understand the canopy surface irregularity and community heterogeneity,identify geometrical characteristics of canopy gaps and construct canopy chemical assemblies from living vegetation volumes.More cooperation between plant ecologists and remote sensing experts is needed to promote UAV remote sensing in advancing plant ecology research. 展开更多
关键词 UAVS DRONES unmanned aircraft systems(UASs) plant ecology species identification community function
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