GF14 widely occurs in plant species functioning as a regulatory protein in cell signal transdution, including monoamine synthesis, exocytosis and cell cycle regulation. A partial sequence, 413 bp in length, is cloned ...GF14 widely occurs in plant species functioning as a regulatory protein in cell signal transdution, including monoamine synthesis, exocytosis and cell cycle regulation. A partial sequence, 413 bp in length, is cloned from the bulbs of F. crrihosa by means of RT PCR technique. Sequence alignment with the BLAST program shows that it is highly similar to GF14 gene. A new full length cDNA, 1133 bp, is obtained by RACE techniques. It contains a major open reading frame of 262 amino acids, 5’and 3’ untranslated regions and a long Poly (A) tail. Sequence analysis of deduced GF14 protein from F. crrihosa indicates several conserved motifs of GF14 proteins are also found in sequence of GF14 protein of F. crrihosa.展开更多
文摘GF14 widely occurs in plant species functioning as a regulatory protein in cell signal transdution, including monoamine synthesis, exocytosis and cell cycle regulation. A partial sequence, 413 bp in length, is cloned from the bulbs of F. crrihosa by means of RT PCR technique. Sequence alignment with the BLAST program shows that it is highly similar to GF14 gene. A new full length cDNA, 1133 bp, is obtained by RACE techniques. It contains a major open reading frame of 262 amino acids, 5’and 3’ untranslated regions and a long Poly (A) tail. Sequence analysis of deduced GF14 protein from F. crrihosa indicates several conserved motifs of GF14 proteins are also found in sequence of GF14 protein of F. crrihosa.
