A normalized full-length cDNA library was constructed from the coralloid roots of Cycas debaoensis by the DSN （duplex-specific nuclease） normalization method combined with the SMART （Switching Mechanism At 5＇ end ...A normalized full-length cDNA library was constructed from the coralloid roots of Cycas debaoensis by the DSN （duplex-specific nuclease） normalization method combined with the SMART （Switching Mechanism At 5＇ end of the RNA Transcript） technique. The titer of the original cDNA library was about 1.5 × 10^6 cfu·mL^-1 and the average insertion size was about 1 kb with a high recombination rate （97%）. The 5011 high-quality expressed sequence tags （ESTs） were obtained from 5393 randomly picked cDNA clones. Clustering and assembly of ESTs resulted in 2984 unique sequences, consisting of 618 contigs and 2366 singlets. EST sequence annotation revealed that 2333 and 1901 unigenes were functionally anno- tated in the NCBI non-redundant database and Swiss-Prot protein database, respectively. Functional analysis demonstrated that 1495 （50.1%） unigenes were associated with 4082 Gene Ontology （GO） terms. A total of 847 unigenes were grouped into 22 Cluster of Orthologous Groups （COG） functional categories. Based on the EST dataset, 22 ESTs that encoded putative receptor-like protein kinase （RLK） genes were screened. Furthermore, a total of 94 simple sequence repeats （SSRs） were discovered, of which 20 loci were successfully amplified in C debaoensis. This study is the first EST analysis for the coralloid roots of C debaoensis and provides a valuable genomic resource for novel gene discovery, gene expression and comparative genomics, conservation and management studies as well as applications in C debaoensis and related cycad species.展开更多
Genista saharae Cosson and Durieu, endemic Saharan Africa, is a spontaneous fabaceae dunes south of M＇sila （Algeria）. Perfectly adapted to arid and sandy soils, this species is a remarkable plant for dune fixation ...Genista saharae Cosson and Durieu, endemic Saharan Africa, is a spontaneous fabaceae dunes south of M＇sila （Algeria）. Perfectly adapted to arid and sandy soils, this species is a remarkable plant for dune fixation and thus fights against desertification. The objective of this study is to provide local species to various development programs of steppe ecological systems in nature and therefore ensure the rehabilitation of these species while considering the conservation of biodiversity and sustainable management of biological resources steppe. After a field survey and stratified sampling, the methodological approach has taken into account three types of analysis： a biometric analysis of a population of 100 individuals of this species, the second analysis on its biology at last an analysis of an ecological factor dealing with the accuracy governing the distribution of this species in Maiter Oued in the south of M＇sila. The results have clarified the ecology of this species to better understand its biology and to develop statistically a diagnosis on the phenology and morphology of various organs （pods, seeds, leaves） of Genista saharae Cosson and Durieu.展开更多
基金supported by the Grant(201522)from Shenzhen Urban Management
文摘A normalized full-length cDNA library was constructed from the coralloid roots of Cycas debaoensis by the DSN （duplex-specific nuclease） normalization method combined with the SMART （Switching Mechanism At 5＇ end of the RNA Transcript） technique. The titer of the original cDNA library was about 1.5 × 10^6 cfu·mL^-1 and the average insertion size was about 1 kb with a high recombination rate （97%）. The 5011 high-quality expressed sequence tags （ESTs） were obtained from 5393 randomly picked cDNA clones. Clustering and assembly of ESTs resulted in 2984 unique sequences, consisting of 618 contigs and 2366 singlets. EST sequence annotation revealed that 2333 and 1901 unigenes were functionally anno- tated in the NCBI non-redundant database and Swiss-Prot protein database, respectively. Functional analysis demonstrated that 1495 （50.1%） unigenes were associated with 4082 Gene Ontology （GO） terms. A total of 847 unigenes were grouped into 22 Cluster of Orthologous Groups （COG） functional categories. Based on the EST dataset, 22 ESTs that encoded putative receptor-like protein kinase （RLK） genes were screened. Furthermore, a total of 94 simple sequence repeats （SSRs） were discovered, of which 20 loci were successfully amplified in C debaoensis. This study is the first EST analysis for the coralloid roots of C debaoensis and provides a valuable genomic resource for novel gene discovery, gene expression and comparative genomics, conservation and management studies as well as applications in C debaoensis and related cycad species.
文摘Genista saharae Cosson and Durieu, endemic Saharan Africa, is a spontaneous fabaceae dunes south of M＇sila （Algeria）. Perfectly adapted to arid and sandy soils, this species is a remarkable plant for dune fixation and thus fights against desertification. The objective of this study is to provide local species to various development programs of steppe ecological systems in nature and therefore ensure the rehabilitation of these species while considering the conservation of biodiversity and sustainable management of biological resources steppe. After a field survey and stratified sampling, the methodological approach has taken into account three types of analysis： a biometric analysis of a population of 100 individuals of this species, the second analysis on its biology at last an analysis of an ecological factor dealing with the accuracy governing the distribution of this species in Maiter Oued in the south of M＇sila. The results have clarified the ecology of this species to better understand its biology and to develop statistically a diagnosis on the phenology and morphology of various organs （pods, seeds, leaves） of Genista saharae Cosson and Durieu.
