浅析植物生理化研究中的DAPI技术

DAPI是最新的DNA荧光染料,目前我国对DAPI技术运用到生物学方面的研究很少。本文将全面的总结DAPI技术运用于植物生理化研究中的成果。结果显示DAPI可以非常清楚的显示植物原本质体细胞核、细胞类核及花粉核的DNA。同时也适用于显微分光光侧研究,希望DAPI技术能够为以后的相关研究提供更大的帮助。

Physiological and Biochemical Response of Artificial Wetland Plant under Electric Field

By measuring wetland plants chlorophyll content,malondialdehyde(MDA) content and superoxide dismutase(SOD) enzyme activity,the changes of wetland plant physiological characeristics under different power strength were studied,and the mechanism of electric field on plant physiological characteristics was analyzed to provide a theoretical basis for the pollutant removal ability strengthening of artificial wetland under electricfield.The results showed that compared with the control plants,low-intensity-voltage(1 V and 3 V) had no significant effect on the normal physiological and biochemical indexes of the plants,and the growth trend was better than the control group;with the voltage increasing,plant chlorophyll content,MDA content and SOD activity were greatly affected,indicating that plants were under strong oxidative stress,and the growth was damaged.Therefore,a suitable electric field could enhance the sewage treatment effect of constructed wetland.

Physiological Mechanism for Anthocyanins to Strengthen the Drought Tolerance of Plants

This paper summarized the possible physiological mechanism by which anthocyanins strengthen the tolerance of plants to drought. Drought stress can in-duce plant cel s to synthesize and accumulate anthocyanins. The photochemical properties, subcel ular accumulation sites and spatial distributions in plant organs and tissues of anthocyanins determine their function of strengthening plant tolerance, which is realized by three possible physiological mechanisms： （1） anthocyanins and their chelated metal ions can optimize the osmoregulation ability of the plant cel s by directly acting as the osmoregulation substances of the cel s, （2） anthocyanins with suitable spatial locations can reduce the photoinhibition of the plants under drought stresses, （3） anthocyanins can effectively maintain and improve the active oxygen-scavenging capacity of the plant cel s under drought conditions. Therein, that the anthocyanins enhance the antioxidant capacity of the plant cel s under drought stresses is probably the main reason for the anthocyanins to strengthen the drought tolerance of plants. This review could provide a reference for the mechanism re-search of the drought resistance and the breeding of the drought-resistant cultivars for the plants holding the ability to synthesize and accumulate anthocyanins.

Effect of vernalization and plant growth regulators on flowering of Onithogalum ‘Chesapeake Snowflake’

The effect of vernalization and two growth regulators Fascination^TM and Pro-Gibb^R on the growth, inflorescence development and flowering of Omithogalurrt Chesapeake Snowflake＇ was studied. Regardless of growth regulator treatment, chilling bulbs for 3 weeks at 10℃ before planting accelerated flowering of the first inflorescence by 5 to 6 days, elongated floral stem length by 1.5 to 2 cm and shortened leaf length by 4 to 5 cm as compared with non-chilled bulbs. When bulbs were chilled 3 weeks at 10℃ before planting, Fascination 2% and Pro-Gibb accelerated flowering of the first inflorescence by 2 to 6 days respectively as compared with the control. When bulbs were not chilled before planting, Fascination 2% and Pro-Gibb accelerated flowering of the first inflorescence by 6 to12 days respectively as compared with the control. The lengths of the leaves and the inflorescences were not affected by PGR treatment. Higher rates of Pro-Gibb （100 mg.L^-1 and 200 mg.L^-1） and more amount of Fascination 2%（100 μL and 200 μL） can cause abortion of inflorescence.

Physiological and Phytosanitary Potentials of Coriander and Radish Seeds

Information on seed vigor of the vegetable crops is always important and necessary due to the increasing demand of high quality seeds for sowing and high-value commercial. The cultivation of these species, conducted intensively, should be established with seed high potential physiological and health for the development of a more productive and sustainable agriculture. The present study was conducted to evaluate the potential physiological of seed lots of radish and coriander. The experiment was conducted at the laboratory of seed analysis and greenhouse of the plant science department of the Federal University of Pelotas in South Brazil. Four radish seed lots, cultivar ＂Saxa＂, and four coriander seed lots, cultivar ＂Verdito＂ were used. Germination seed test, first count of germination, accelerated aging test, electrical conductivity and seedling emergence were used to evaluate the physiological quality of the lots. The experimental design was completely randomized design with five replications. Means were compared by Tukey test. The accelerated aging test was the most efficient test in assessing the physiological quality for both lots of radish seeds and coriander and this test provide coherent results with seedlings emergence.