Vernonia amygdalina Del. is a tropical species susceptible to frost which is an annual phenomenon of the winter season of the Eastern Cape. Limited populations of the species arc in the province dieback during the win...Vernonia amygdalina Del. is a tropical species susceptible to frost which is an annual phenomenon of the winter season of the Eastern Cape. Limited populations of the species arc in the province dieback during the winter period, thereby making large scale cultivation through conventional vegetative propagation impossible. In our effort to increasing the population of the species within the province, a micropropagation approach through tissue culture teehnology was employed. Two growth regulators （BAP ＆ NAA） were tested at four levels of concentration under continuous light and dark conditions. Plantlets were subsequently transferred to the field after acclimatization at different temperature conditions. The result of the study showed that BAP generally performs better than NAA for callus induction under continuous darkness, while direct micropropagation of shoot under continuous light condition at I mg L^-1BAP showed the best result. To achieve greater success for the rapid multiplication of the species, this study further demonstrates that the optimum temperature range for acclimatizing the species prior to the transfer of the plantlet to the field is between 23 ±3 ℃.展开更多
Plum is the major fruit species in the area of North Montenegro. Over a long period of growing in this region, autochthonous cultivars adapted, and have been achieving satisfactory results, despite poor growing condit...Plum is the major fruit species in the area of North Montenegro. Over a long period of growing in this region, autochthonous cultivars adapted, and have been achieving satisfactory results, despite poor growing conditions. A study conducted over a period of three years in a North Montenegro region included in situ identification of autochthonous plum cultivars. Observation and recording of their phenological and pomological traits were performed using International Board for Plant Genetic Resources （IBPGR） and International union for the Protection of New Varieties of plants （UPOV） methodologies. Eighteen cultivars derived from Prunusdomestica L. and two cultivars derived from P. insititia L. were identified. Flowering started between March 26th and April 12th and fruit ripening between 13th of July （Petrovaca） and 18th September （Trnovaca）. Fruit weight ranged from 6.65 g ± 0.235 g to 53.88 g ± 0.654 g respectively and stone weight from 0.16 g ±0.003 g to 2.20 g ± 0.711 g. The cultivars were classified as being extremely small in terms of fruit size, except for cvs Crvenadurgulja （big fruit size）. Rounded fruit shape and light green ground color were dominant. Skin color ranged from amber to black. Yellow green was a dominant flesh color and medium flesh firmness predominated. The fruits of the above cultivars could be processed, particularly into plum brandy, or they could be used fresh or dried. The selected plum cultivars can be used in breeding programmes, as rootstocks as well as in further disease related systematic studies under field and laboratory conditions.展开更多
文摘Vernonia amygdalina Del. is a tropical species susceptible to frost which is an annual phenomenon of the winter season of the Eastern Cape. Limited populations of the species arc in the province dieback during the winter period, thereby making large scale cultivation through conventional vegetative propagation impossible. In our effort to increasing the population of the species within the province, a micropropagation approach through tissue culture teehnology was employed. Two growth regulators （BAP ＆ NAA） were tested at four levels of concentration under continuous light and dark conditions. Plantlets were subsequently transferred to the field after acclimatization at different temperature conditions. The result of the study showed that BAP generally performs better than NAA for callus induction under continuous darkness, while direct micropropagation of shoot under continuous light condition at I mg L^-1BAP showed the best result. To achieve greater success for the rapid multiplication of the species, this study further demonstrates that the optimum temperature range for acclimatizing the species prior to the transfer of the plantlet to the field is between 23 ±3 ℃.
文摘Plum is the major fruit species in the area of North Montenegro. Over a long period of growing in this region, autochthonous cultivars adapted, and have been achieving satisfactory results, despite poor growing conditions. A study conducted over a period of three years in a North Montenegro region included in situ identification of autochthonous plum cultivars. Observation and recording of their phenological and pomological traits were performed using International Board for Plant Genetic Resources （IBPGR） and International union for the Protection of New Varieties of plants （UPOV） methodologies. Eighteen cultivars derived from Prunusdomestica L. and two cultivars derived from P. insititia L. were identified. Flowering started between March 26th and April 12th and fruit ripening between 13th of July （Petrovaca） and 18th September （Trnovaca）. Fruit weight ranged from 6.65 g ± 0.235 g to 53.88 g ± 0.654 g respectively and stone weight from 0.16 g ±0.003 g to 2.20 g ± 0.711 g. The cultivars were classified as being extremely small in terms of fruit size, except for cvs Crvenadurgulja （big fruit size）. Rounded fruit shape and light green ground color were dominant. Skin color ranged from amber to black. Yellow green was a dominant flesh color and medium flesh firmness predominated. The fruits of the above cultivars could be processed, particularly into plum brandy, or they could be used fresh or dried. The selected plum cultivars can be used in breeding programmes, as rootstocks as well as in further disease related systematic studies under field and laboratory conditions.
