不同植物生长调节剂对冬小麦生长及产量的影响

为考察不同植物生长调节剂对农作物生长状况及产量的影响,以氨基寡糖素、复硝酚钠和胺鲜酯为处理药剂,以复合肥(18-15-12)为药剂载体,于冬小麦播种前底施于土壤,测定了不同生育时期冬小麦的株高、主根长、须根数、分蘖数、叶片数、顶节长、穗长、旗叶长、颖果数等指标。结果表明:氨基寡糖素在冬小麦前期能促苗生根,中期能促叶、增加分蘖数,提高冬小麦产量;复硝酚钠主要表现在中后期能促进冬小麦株高和旗叶的生长;胺鲜酯在冬小麦前期对茎叶、须根数,在中期对顶节长、颖果数具有促进作用,但后期对顶节长、旗叶长和颖果数有抑制作用;复硝酚钠和胺鲜酯均会降低冬小麦产量。

不同植物生长调节剂浸种对大豆幼苗子叶碳代谢的影响

在砂培条件下，以清水浸种为对照（CK），以不同浓度己酸二乙氨基乙醇酯（DTA-6）和烯效唑（S-3307）浸种为处理，研究不同浓度的DTA-6和S-3307对苗期大豆子叶内可溶性糖、淀粉、蔗糖及还原糖含量的影响。结果表明：处理和对照的可溶性糖含量均呈升高-下降-升高的变化趋势，出苗后第6天DTA-6处理的可溶性糖含量低于S-3307处理和CK；淀粉含量呈先减少后增加的变化趋势，在出苗后第8天降到最低点，出苗后第10～12天子叶淀粉含量又有所增加，出苗后第12天DTA-6处理的淀粉含量低于CK和S-3307处理；子叶蔗糖的含量大致呈升高-降低-升高的趋势，S-3307处理的蔗糖含量普遍高于DTA-6处理；子叶还原糖含量在取样时间内出现了几次波动，其中苗后第8天左右减少到较低值，DTA-6普遍提高了还原糖含量，S-3307（S0．2除外）降低了取样后期的还原糖含量。综合分析表明：适宜浓度的植物生长调节剂浸种能够调控子叶的碳代谢，利于幼苗的健壮生长，2类调节剂中分别以D50和S0．2的调控效果最佳。

近几年我国植物生长调节剂登记概述

植物生长调节剂在我国农业可持续发展中发挥了较大的积极作用。本文概述了我国植物生长调节剂作为农药一类的登记管理的法律依据、基本程序及有关生物学资料要求,还重点介绍了我国近几年的基本登记情况、部分新产品、存在问题,并对我国植物生长调节剂的今后发展进行了展望。

Influence of Plant Growth Regulators on Pollen Germination and Growth of Xinjiang Apricots

[Objective] This study aimed to investigate the influence of different plant growth regulators on apricot pollen germination and pollen tube growth. [Method] Pollens of six kinds of Xinjiang apricots were cultured in solid media supplemented with five plant growth regulators （GA3 , NAA, 2, 4-D, 6-BA, IAA）. Then the rate of pollen germination and the length of pollen tube were respectively measured. [Result] In a certain concentration range, GA3 most significantly promoted the pollen germination and the pollen tube growth of Shushanggan, Kalayulvke, Dayoujia, Yiliakeyulvke and Kabakehuanna; NNA had the strongest improvement function on Kumaiti’s pollen germination and pollen tube growth. [Conclusion] All the five plant growth regulators promoted the pollen germination and the pollen tube growth of apricots at low concentration but inhibited them at high concentration.

Dwarfing effects of plant growth regulators on narcissi

The effects of four kinds of plant growth regulators with different concentrations on narcissi were studied in 2001. The results showed that the regulators could inhibit the growths of height and leaves of narcissi. Of the four regulators, the dwarfing effects of paclobatrazol (PP333) and uniconazole (S3307) on narcissi were better than those of chlorocholine (CCC) and dimethyl amino-sussinamic acid (B9). All of the regulators did not have significant effect on the root length. Moreover, the time of flowering was later for the narcissi treated with regulators than that of the control to a certain extent, and the range delayed was from 2 days to 19 days. The correlation analysis results showed that there was a significant correlation between the time of flowering and the concentrations of regulators. The ornament value of narcissi was obviously improved by using the regulators.

Effects of Plant Growth Regulator on Absorption,Circulation and Contents of Potassium in Flue-cured Tobacco

[Objective] This study was to investigate the effects of plant growth regulator on accumulation and circulation of potassium in flue-cured tobacco.[Methods] Hydroponics experiment was adopted to study the effects of plant growth regulator（NAA,GA3,BR） on accumulation and circulation of potassium in flue-cured tobacco.[Results] The BR treatment increased the absorption of potassium,calcium and magnesium in flue-cured tobacco,reduced the potassium emission from the root,enhanced the accumulation and contents and promoted the circulation of potassium in the tobacco plants.There was no statistical difference between the NAA and GA3 treatment.And BR treatment was 0.52%,0.30%,0.28% higher than NAA treatment in enhancing potassium content in tobacco plants at 2,7,12 d after topping.In a word,the results showed that BR treatment was the most.[Conclusion] BR treatment could effectively enhance potassium content in tobacco plants after topping.

库尔勒香梨外观品质影响因素研究

研究了树冠不同结果部位、果实饱满种子数和盛花期喷布植物生长调节剂对库尔勒香梨外观品质的影响。结果表明,果实饱满种子数与库尔勒香梨果形有关,果实脱萼洼顶、平均单果重113.0g、果形指数1.15,是库尔勒香梨果实的理想指标;相比于树冠顶部,树冠下部的库尔勒香梨果实较理想,以树冠下部东面为最佳结果部位;盛花期喷布多效唑、乙烯利有利于生产理想果实,建议不喷布赤霉素、吡效隆和生长素。

Influences of Carbon Sources and Plant Growth Regulators on Anther Culture Efficiency of Pepper

[Objective]In order to increase anther culture efficiency of pepper.[Method]MS culture media and Bolajiaohong were used in this experiment to study the influences of carbon sources and concentrations on anther callus induction of pepper.Jiayu was taken as a material to study influences of plant growth regulators and concentrations on anther callus induction of pepper according to L16（4^5） orthogonal design.[Result]The average callus and embryoid induction rates of maltose at all concentrations were higher than these of sucrose but the difference was not significant.Taking maltose or sucrose as a carbon source,3% to 6% concentration was good for increasing induction frequencies of calli and embryoids.However,If the concentration was over 6%,the induction rates were declined dramatically with the increase of sugar concentration.The influences of growth regulators on induction rate of calli were listed as 2,4-D﹥ZT﹥NAA﹥KT﹥6-BA;the influences on induction rates of embryoids were listed as 2,4-D﹥NAA﹥ZT﹥KT﹥6-BA.The 2,4-D,ZT,NAA and KT had signficant or extremely significant influences on induction rates of calli and embryoids.2,4-D,ZT at 1.0 mg/L and NNA,KT at 0.5 mg/L had the best effects.The influences of ZT on calli and embryoids were better than those of KT and 6-BA.1.0 mg/L 2,4-D ＋1.0 mg/L ZT ＋0.5 mg/L KT ＋0.5 mg/L 6-BA was the best regulator combination for induction culture of Jiayu anther.[Conclusion]The experiment provided research basis for anther culture of pepper.

Effect of vernalization and plant growth regulators on flowering of Onithogalum ‘Chesapeake Snowflake’

The effect of vernalization and two growth regulators Fascination^TM and Pro-Gibb^R on the growth, inflorescence development and flowering of Omithogalurrt Chesapeake Snowflake＇ was studied. Regardless of growth regulator treatment, chilling bulbs for 3 weeks at 10℃ before planting accelerated flowering of the first inflorescence by 5 to 6 days, elongated floral stem length by 1.5 to 2 cm and shortened leaf length by 4 to 5 cm as compared with non-chilled bulbs. When bulbs were chilled 3 weeks at 10℃ before planting, Fascination 2% and Pro-Gibb accelerated flowering of the first inflorescence by 2 to 6 days respectively as compared with the control. When bulbs were not chilled before planting, Fascination 2% and Pro-Gibb accelerated flowering of the first inflorescence by 6 to12 days respectively as compared with the control. The lengths of the leaves and the inflorescences were not affected by PGR treatment. Higher rates of Pro-Gibb （100 mg.L^-1 and 200 mg.L^-1） and more amount of Fascination 2%（100 μL and 200 μL） can cause abortion of inflorescence.

Efficient in vitro Propagation of an Endangered Species Kolkwitzia amabilis Graebn

This study developed an efficient in vitro cultivation and propagation sys- tem for an endangered species Kolkwitzia amabilis using nodal segments as ex- plants. Multiple shoots were induced through axillary bud formation. The highest fre- quency of multiple shoot induction was achieved when the nodal segment explants were incubated in Murashige and Skoog （MS） medium supplemented with 4.44 pM 6-benzyladenine （6-BA） in combination with 0.54 μM a-naphthaleneacetic acid （NAA）, followed by treatment with 4.44 μM 6-BA in combination with 0.27 μM NAA. Shoot multiplication could be induced in MS medium supplemented with stand-alone 6-BA or 6-BA in combination with indole-3-acetic acid （1.71 μM） or NAA （0.27 or 0.54 μM）, with 6-BA and either compound, exhibiting a stronger effect on shoot multiplication. The optimum combination of plant growth regulators for shoot multiplication was 4.44 μM 6-BA with 0.27 μM NAA. The maximum rooting percentage was obtained in a half-strength MS medium supplemented with indole-3-butyric acid alone and in com- bination with NAA and 2,4-dichlorophenoxyacetic acid, but the best combination of plant growth regulators for rooting was 1.48 μM indole-3-butyric acid with 1.08 μM NAA and 0.05 μM 2,4-dichlorophenoxyacetic acid. The rooted shoots were trans- ferred to a greenhouse with a success rate of 100%.

Effects of Plant Growth Regulators on the Contents of Reducing Sugar and Starch in Potato(Solanum tuberosum L.)

[Objective] The aim was to investigate the effects of plant growth regulators on contents of reducing sugar and starch in potato（Solanum tuberosum L.）.[Method]A potato cultivar named ＇Holand-212＇ with three treatments by spraying Diethyl aminoethyl hexanoate（DTA-6）,Uniconazole（S3307）and SOD mimics（SODM）were employed to compare differences of the contents of reducing sugar and starch in potato leaves,stolons and tubers in a field experiment.[Result]DTA-6 treatment could significantly reduce the contents of starch and reducing sugar in potato stolons in the period of 100-110 d after seeding,and it could also reduce the content of reducing sugar in tubers,but there is an opposite result in leaves in the same period,significantly.S3307 could extremely significantly increase the content of starch,and can promote the transfer of starch in stolons,in addition,it also could reduce the content of reducing sugar in tubers,and extremely significantly increase the content of starch in leaves at the 110 d after sowing.Moreover,there is a similar effect between SODM and S3307.[Conclusion]The research results are significant for understanding mechanisms of plant growth regulators and promoting application of plant growth regulator in potato.

Arabidopsis RAV1 is down-regulated by brassinosteroid and may act as a negative regulator during plant development

RAV1 is a novel DNA-binding protein with two distinct DNA-binding domains unique in higher plants,but its role in plant growth and development remains unknown. Using cDNA array,we found that transcription of RAV1 is downregulated by epibrassinolide (epiBL) in Arabidopsis suspension cells. RNA gel blot analysis revealed that epiBL-regulated RAV1 transcription involves neither protein phosphorylation/dephosphorylation nor newly synthesized protein,and does not require the functional BRI1,suggesting that this regulation might be through a new BR signaling pathway.Overexpressing RAV1 in Arabidopsis results in a retardation of lateral root and rosette leaf development,and the underexpression causes an earlier flowering phenotype,implying that RAV1 may function as a negative regulatory component of growth and development.

Progress of safflower (Carthamus tinctorius L.) regeneration through tissue culture

Background: Safflower regeneration through tissue culture has long been limited to low frequency and lack of an efficient protocol that suitable for most safflower cultivars. In past decades, researches had been carried out to investigate safflower regeneration through tissue culture and great progress had been made. Objective: To investigate factors that affect safflower regeneration through tissue culture principally. Methods: This article summarized available literatures about advancements in safflower regeneration, especially discussed factors affecting safflower tissue culture in detail. Results: Safflower regeneration was fairly hard than other congeneric plants, such as chrysanthemum. The genotype, seedling age, type of explants, medium components, plant growth regulators and other additives all had specific influences on safflower tissue culture. More deepgoing researches need to be undertaken to establish an effective safflower regeneration system.

Effect of Different Plant Growth Regulators on Callus Induction and in vitro Rapid Propagation of Wild Petunia Juss.

In this study,the seeds of wild Petunia Juss.were used as explants to investigate the optimal condition for tissue culture.Several different kinds and concentrations of growth regulators were adopted to produce more multiple bud clumps,callus or roots in this study.The experiments may provide experimental foundation for the rapid propagation technology and establishment of tissue culture system for wild Petunia Juss.

Synthesis and Biological Evaluation of Novel N-Benzoxazolonyluracils as Protox-Inhibitor

Protoporphyrinogen oxidase inhibitor has become the focus research field as herbicides.Seven novel N-benzoxazolonyluracils were obtained by cyclization of 6-aminobenzoxazolone derivatives with ethyl 3-dimethylcarbamoylamido-4,4,4-trifluorocrotonate and then methylation or amination, and the reaction yields were between 5900—8600. The chemical structures of the compounds were identified by Elemental Analysis, MS and 1H NMR. Biological results indicate that some compounds exhibit excellent control of broadleaf weeds at post-emergence by low dosage 18.75 ga.i/ha, but are not effective for grass weeds below dosage 37.5 ga.i/ha. In addition, these compounds show no damage at high-dosage 150 ga.i/ha to wheat and corn, but are unsafe to dicotyledonous plants such as cole, soybean and sorghum even at lower dosage 18.75 ga.i/ha.

High Efficiency of Propagation for Sagittaria sagittifolia Using a Temporary Immersion Bioreactor System

Although several studies have reported on the propagation of the Sagittaria sagittifolia to date, none of these methods have efficiently achieved the mass production of these plants. The present study aimed to investigate the propagation and growth of S. sagittifolia using a temporary immersion bioreactor system （TIBS） compared with conventional semi-solid and liquid culture. The effect of different immersion frequencies and immersion times together with supplementation of various plant growth regulators to the Murashige and Skoog （MS） medium was evaluated on shoot proliferation and plant growth. The results showed that the higher immersion frequency （every 6 h） and shorter immersion time （3 min and 10 rain） in medium containing 4 mg/L BA and 0.1 mg/L NAA produced the highest multiplication rate （23）, which are significantly higher than conventional semi-solid （3.6） and liquid （4.5） method, and the best plant growth parameter. While, the lower immersion frequency and longer immersion time （30 rain every 12 h and 60 min every 24 h） induced vitrification and pollution rate in shoot tips 16.6% and 19%, 42% and 37%, respectively. There is distinct decrease in pollution rate （8.3%） in TIBS （10 min every 6 h） compared with the conventional semi-solid and liquid cultures. Medium containing 4 mg/L BA and 0.5 mg/L NAA using 10 min immersion every 6 h showed satisfaction at the rooting stage, with high shoot proliferation rate （21.6）, 100% rooting and 94% plant survival. Therefore, applying TIBS in S. sagittifolia is an efficient method for scaling up the production of plantlets with high quality seedlings.

Development of an Efficient Plant Regeneration System for Pelargonium×Citrosum Vanleenii

[Objective]As a mosquito-repelling ornamental plant,Pelargonium×Citrosum Vanleenii(P.× Citrosum Vanleenii)is hard to be acquired because of its hybrid background,the paper was to a new regeneration system of(P.× Citrosum Vanleenii).[Method] By studying the influence of plant growth regulators(PGRs)on explant type(leaves and petioles),the optimal combinations of PGRs to maximize SELSs(somatic embryo-like structure)and buds were established.[Result]0.2 mg/L NAA+1.0 mg/L BA was best for LS(leaves segments)and 0.2 mg/L NAA + 1.5 mg/L BAs was best for PS(petioles segments).Cultured plantlets were successfully acclimatized in soil where they grew normally without any morphological variation.Although both LS and PS were usable,the leaf was a better explant for induction of embryogenic calli,somatic embryo-like structures and buds.[Conclusion]This work offered a rapid and efficient system for plant regeneration of P.×Citrosum Vanleenii.

Conservation of Iris bismarckiana Regel （Iridaceae） Using Plant Regeneration via Somatic Embryogenesis

In an attempt to propagate and conserve the rare, showy bulbous plants of Iris bismarckiana, newly recorded to the flora of Jordan and to contribute to the conservation the wild lris species in Jordan, a simple rapid, time consuming protocol has been developed using plant regeneration via somatic embryogenesis. Somatic embryogenesis was induced in zygotic embryo culture on Murashige and Skoog （MS） medium supplemented with 2,4-dichlorophenoxy acetic acid （8 mg/L） as the sole plant growth regulator, where both embryogenesis calli and somatic embryos were induced. Separation of embryos from embryo clusters was necessary to enhance the frequency of germination. Germination was stimulated by separation of embryos successfully from embryo clusters and transferred onto fresh MS medium. Data obtained were analyzed as a complete random design with three replications. Calli fragments that were transferred to embryogenesis induction medium （EIM） produced white embryo-like globular structures within two weeks. Within three more weeks, clusters of structures at various stages of development could be found on the same callus. The applied technique is rewarding and encouraging for further research on the endangered wild species of Iris in Jordan.

New Plant Growth Regulator Emistim C AS used on Watermelon in Field Evaluation

Most plant growth regulators available are synthesized and tend to be dangerous if used inappropriately. Emistim CAS is a natural plant growth regulator, with less side-effect. The research performed an assessment on Emistim CAS application in watermelon cultivation in a greenhouse. The results showed that 0.11% Emistim CAS increased reducing sugar by 15.49%, soluble solid by 13.7%, Vc content by 3.72%, titratable acid by 18.1%, and yield per hectare by 18.01%, so that watermelon quality and yield were both improved. Emistim CAS can be widely promoted in watermelon cultivation in future.

Chlorocholine Chloride Induces Cacao Reproductive Development Leading to Improved Fruitlets Productivity of Cacao Trees in the Field

Fruitlet abscission （cherelle wilt） is a major factor contributing to lower productivity of cacao plantation in Indonesia. An attempt was made to reduce such cherelle wilt by spraying plant growth retardants （PGRs） on cacao trees at about 10 weeks before flowering session. The treatment was repeated every three weeks. Both the flowering initiation time and the number of flowers on the treated trees were improved significantly. With the most effective composition, flowers were initiated at 24 days after spraying （DAS）, where as the flowering initiation was 11-day later in the controls. At the 35 DAS, the chlorocholine chloride （CCC） 2,000 treatment induced about 20-80 folds more flowers than the controls. Extended observation was made to assess the treatment effect on the fruiting capacity. The treatments were found to improve fruiting ability by increasing the number of fruits set on the trees and shortening the time for fruit setting. At 20 weeks after the first spraying （WAS） there were on average 12.57 fruits per CCC 2,000-treated tree, as opposed to only 4.14 fruits per untreated tree. Analyses of the metabolites content in the flower cushions of the trees indicated that the reproductive growth is significantly correlated with the increased metabolites particularly the reduced sugar. Exogenous addition of sucrose to the PGR treatment gave greater improvement mainly in the fruiting. In conclusion, foliar spray of CCC induces flowering of cacao tress in the field that leads to improve the fruitlets productivity.