In order to reveal the signaling pathways triggered by elicitor in plant-microbe interactions, the mechanisms of hypersensitive necrosis responses in Nicotiana tabacum L. cv. Gexin III induced by palmin were studied a...In order to reveal the signaling pathways triggered by elicitor in plant-microbe interactions, the mechanisms of hypersensitive necrosis responses in Nicotiana tabacum L. cv. Gexin III induced by palmin were studied at molecular and cellular level. The burst of superoxide, intercellular diffusion of hydrogen peroxide and process of cell death induced by palmin were investigated in tobacco plants by biochemical methods and Confocal microscopy. The results showed that a large amount of O-2(.-) was rapidly generated in tobacco cell elicited by palmin as a result of activation of NADPH oxidase, and the O-2(.-) was dismutated into H2O2 immediately by superoxide dismutase (SOD). Accumulation and intercellular diffusion of H2O2 were shown to be a trigger for hypersensitive cell death; and Ca2+ and some specific protein kinase were also shown to be involved in the activation of oxidative burst in tobacco cell induced by palmin.展开更多
It is observed contamination and subsequent growth of various types of mycotoxins in the production and processing of grain and non-grain crops. The contamination of grain and non-grain cereals crops harvest was analy...It is observed contamination and subsequent growth of various types of mycotoxins in the production and processing of grain and non-grain crops. The contamination of grain and non-grain cereals crops harvest was analyzed. The aim of this research is using of microwave energy to disinfect grains of harvest and giving new properties to the grains and plants materials. The author has presented researches of the grains disinfection, during seedbed preparation and post processing. Rational parameters of heating rates of different biological objects were identified, revealed their dependence and impact on infection pathogens, through using of microwave energy technology. The author found a reduction of the number of pathogenic microbes and organisms at the various stages of processing agricultural products during using of microwave energy, and found new qualitative indicators of the products properties.展开更多
This study presents the antimicrobial effect of natural surface secretes of some common ornamental plants leaves (Ficus elastica, Philodendrom bipinnatifidum and Aglonema) against some pathogenic bacteria ( Escheri...This study presents the antimicrobial effect of natural surface secretes of some common ornamental plants leaves (Ficus elastica, Philodendrom bipinnatifidum and Aglonema) against some pathogenic bacteria ( Escherichia coli, P. aeruginosa and Staph. Aureus), and fungi (Microsporum gypseum and Aspergillus flavus). It was concluded that all tested washing water of the plants leaves secretes exhibited various inhibitory effects, both Philodendrom more antibacterial activity than Ficus elastica. While both E. coli and P bipinnatifidum and Aglonema commutatum had exhibited aeruginosa were being more sensitive than Staph. aureus. The same inhibitory effects were observed when the plants leaves inoculated in their surface with pathogenic bacteria. In contrast to bacterial inhibitory effects, the washing water of natural surface secretes of tested ornamental plants leaves induced mycelium growth of both tested fungi. Microsporum gypseum mycelium growth induced more than Aspergillusflavus especially in case of the washing water of Ficus elastic that have highest effects at 7.5/500 mL of medium. This study concluded the uses of ornamental plants for the indoors and outdoors to control the growth of pathogenic microbes and problems associated with hospital.展开更多
Soil biotic communities can strongly impact plant performance.In this paper,we ask the question:how longlasting the effect of the soil microbial community on plant growth is.We examined the plant growth rates at three...Soil biotic communities can strongly impact plant performance.In this paper,we ask the question:how longlasting the effect of the soil microbial community on plant growth is.We examined the plant growth rates at three stages:early,mid and late growth.We performed two growth experiments with Jacobaea vulgaris,which lasted 49 and 63 days in sterilized soil or live soil.In a third experiment,we examined the effect of the timing of soil inoculation prior to planting on the relative growth rate of J.vulgaris with four different timing treatments.In all experiments,differences in biomass of plants grown in sterilized soil and live soil increased throughout the experiment.Also,the relative growth rate of plants in the sterilized soil was only significantly higher than that of plants in the live soil in the first two to three weeks.In the third experiment,plant biomass decreased with increasing time between inoculation and planting.Overall,our results showed that plants of J.vulgaris grew less well in live soil than in sterilized soil.The negative effects of soil inoculation on plant mass appeared to extend over the whole growth period but arise from the negative effects on relative growth rates that occurred in the first weeks.展开更多
文摘In order to reveal the signaling pathways triggered by elicitor in plant-microbe interactions, the mechanisms of hypersensitive necrosis responses in Nicotiana tabacum L. cv. Gexin III induced by palmin were studied at molecular and cellular level. The burst of superoxide, intercellular diffusion of hydrogen peroxide and process of cell death induced by palmin were investigated in tobacco plants by biochemical methods and Confocal microscopy. The results showed that a large amount of O-2(.-) was rapidly generated in tobacco cell elicited by palmin as a result of activation of NADPH oxidase, and the O-2(.-) was dismutated into H2O2 immediately by superoxide dismutase (SOD). Accumulation and intercellular diffusion of H2O2 were shown to be a trigger for hypersensitive cell death; and Ca2+ and some specific protein kinase were also shown to be involved in the activation of oxidative burst in tobacco cell induced by palmin.
文摘It is observed contamination and subsequent growth of various types of mycotoxins in the production and processing of grain and non-grain crops. The contamination of grain and non-grain cereals crops harvest was analyzed. The aim of this research is using of microwave energy to disinfect grains of harvest and giving new properties to the grains and plants materials. The author has presented researches of the grains disinfection, during seedbed preparation and post processing. Rational parameters of heating rates of different biological objects were identified, revealed their dependence and impact on infection pathogens, through using of microwave energy technology. The author found a reduction of the number of pathogenic microbes and organisms at the various stages of processing agricultural products during using of microwave energy, and found new qualitative indicators of the products properties.
文摘This study presents the antimicrobial effect of natural surface secretes of some common ornamental plants leaves (Ficus elastica, Philodendrom bipinnatifidum and Aglonema) against some pathogenic bacteria ( Escherichia coli, P. aeruginosa and Staph. Aureus), and fungi (Microsporum gypseum and Aspergillus flavus). It was concluded that all tested washing water of the plants leaves secretes exhibited various inhibitory effects, both Philodendrom more antibacterial activity than Ficus elastica. While both E. coli and P bipinnatifidum and Aglonema commutatum had exhibited aeruginosa were being more sensitive than Staph. aureus. The same inhibitory effects were observed when the plants leaves inoculated in their surface with pathogenic bacteria. In contrast to bacterial inhibitory effects, the washing water of natural surface secretes of tested ornamental plants leaves induced mycelium growth of both tested fungi. Microsporum gypseum mycelium growth induced more than Aspergillusflavus especially in case of the washing water of Ficus elastic that have highest effects at 7.5/500 mL of medium. This study concluded the uses of ornamental plants for the indoors and outdoors to control the growth of pathogenic microbes and problems associated with hospital.
文摘Soil biotic communities can strongly impact plant performance.In this paper,we ask the question:how longlasting the effect of the soil microbial community on plant growth is.We examined the plant growth rates at three stages:early,mid and late growth.We performed two growth experiments with Jacobaea vulgaris,which lasted 49 and 63 days in sterilized soil or live soil.In a third experiment,we examined the effect of the timing of soil inoculation prior to planting on the relative growth rate of J.vulgaris with four different timing treatments.In all experiments,differences in biomass of plants grown in sterilized soil and live soil increased throughout the experiment.Also,the relative growth rate of plants in the sterilized soil was only significantly higher than that of plants in the live soil in the first two to three weeks.In the third experiment,plant biomass decreased with increasing time between inoculation and planting.Overall,our results showed that plants of J.vulgaris grew less well in live soil than in sterilized soil.The negative effects of soil inoculation on plant mass appeared to extend over the whole growth period but arise from the negative effects on relative growth rates that occurred in the first weeks.