Endophytes are beneficial microbes that are capable of promoting growth, besides protecting colonized plants against plant pathogens. These microbes are of either bacterial, fungal or actinomycetes in plants. In the s...Endophytes are beneficial microbes that are capable of promoting growth, besides protecting colonized plants against plant pathogens. These microbes are of either bacterial, fungal or actinomycetes in plants. In the study, the endophytic bacteria isolated from sugarcane with their characterization related to plant growth promotion and pathogen suppression have been reported. Roots, shoots and leaves of rooted tissue culture plantlets of sugarcane cultivars of 87A298 and 2009A107 were excised aseptically and isolated endophytic bacterial strains. The strains were identified using 16S rRNA gene sequence based homology. Molecular characterization of these strains was carried out for presence of antimicrobial genes. The results showed that the endophytes isolated from sugarcane tissue culture plantlets were of the genera Bacillus （B. amyloliquefaciens, B. subtilis, B. cereus, B. safensis, B. siamensis, B. aryabhattai, B. flexus and B. velezensis） and Paenibacillus pabuli. There were three antimicrobial peptides （AMPs） producing genes of bacilysin, bacillomycin and fengycin in B. amyloliquefaciens （SE1, SE7）, B. siamensis （SE4, SE16）, B. subtilis （SE2, SE3） and B. velezensis （SE15）. The biochemical characterization assays showed that some of these strains could produce hydrogen cyanide （HCN）, protease, cellulase and indole acetic acid （IAA）. Few strains （SE1 and SE4） were phosphate solubilizers, whereas nine isolates were found to be diazotrophs. Most of the bacterial isolates were found antagonistic to Fusarium sacchari, the sugarcane wilt pathogen under in vitro conditions. Overall, the results suggested the scope and potentiality of sugarcane endophytic bacteria, isolated from tissue culture plantlets, in promoting plant growth and suppression of sugarcane pathogen.展开更多
Ralstonia solanacearum is an important model phytopathogenic bacterium that causes bacterial wilt disease on many plant species and leads to serious economic losses. The interactions between R. solanacearum and host p...Ralstonia solanacearum is an important model phytopathogenic bacterium that causes bacterial wilt disease on many plant species and leads to serious economic losses. The interactions between R. solanacearum and host plants have become a model system for the study of plants and pathogens interactions. This paper reviews the advances on the molecular mechanisms between R. solanacearum and hosts interaction including the formation of plant innate immunity, the suppression of plant innate immunity by this pathogen and the activation of effector-triggered immunity. Furthermore, we made a prospect on how to utilize the interaction mechanism between R. solanacearum and hosts to control the disease.展开更多
Growing evidence indicates that actin cytoskeleton is involved in plant innate immune responses, but the functional mechanism remains largely unknown. Here, we investigated the behavior of a cotton profilin gene （GhP...Growing evidence indicates that actin cytoskeleton is involved in plant innate immune responses, but the functional mechanism remains largely unknown. Here, we investigated the behavior of a cotton profilin gene （GhPFN2） in response to Verticillium dahliae invasion, and evaluated its contribution to plant defense against this soil-borne fungal pathogen. GhPFN2 expression was up-regulated when cotton root was inoculated with V. dahliae, and the actin architecture was reorganized in the infected root cells, with a clear increase in the density of filamentous actin and the extent of actin btmdling. Compared to the wild type, GhPFN2-overexpressing cotton plants showed enhanced protection against V. dahliae infection and the actin cytoskeleton organization in root epidermal cells was clearly altered, which phenocopied that of the wild-type （WT） root cells challenged with V. dahliae. These results provide a solid line of evidence important for defense against V. dahliae infection. showing that actin cytoskeleton reorganization involving GhPFN2 is展开更多
文摘Endophytes are beneficial microbes that are capable of promoting growth, besides protecting colonized plants against plant pathogens. These microbes are of either bacterial, fungal or actinomycetes in plants. In the study, the endophytic bacteria isolated from sugarcane with their characterization related to plant growth promotion and pathogen suppression have been reported. Roots, shoots and leaves of rooted tissue culture plantlets of sugarcane cultivars of 87A298 and 2009A107 were excised aseptically and isolated endophytic bacterial strains. The strains were identified using 16S rRNA gene sequence based homology. Molecular characterization of these strains was carried out for presence of antimicrobial genes. The results showed that the endophytes isolated from sugarcane tissue culture plantlets were of the genera Bacillus （B. amyloliquefaciens, B. subtilis, B. cereus, B. safensis, B. siamensis, B. aryabhattai, B. flexus and B. velezensis） and Paenibacillus pabuli. There were three antimicrobial peptides （AMPs） producing genes of bacilysin, bacillomycin and fengycin in B. amyloliquefaciens （SE1, SE7）, B. siamensis （SE4, SE16）, B. subtilis （SE2, SE3） and B. velezensis （SE15）. The biochemical characterization assays showed that some of these strains could produce hydrogen cyanide （HCN）, protease, cellulase and indole acetic acid （IAA）. Few strains （SE1 and SE4） were phosphate solubilizers, whereas nine isolates were found to be diazotrophs. Most of the bacterial isolates were found antagonistic to Fusarium sacchari, the sugarcane wilt pathogen under in vitro conditions. Overall, the results suggested the scope and potentiality of sugarcane endophytic bacteria, isolated from tissue culture plantlets, in promoting plant growth and suppression of sugarcane pathogen.
基金Supported by Fundamental Research Funds for the Central Universities(110201202002)
文摘Ralstonia solanacearum is an important model phytopathogenic bacterium that causes bacterial wilt disease on many plant species and leads to serious economic losses. The interactions between R. solanacearum and host plants have become a model system for the study of plants and pathogens interactions. This paper reviews the advances on the molecular mechanisms between R. solanacearum and hosts interaction including the formation of plant innate immunity, the suppression of plant innate immunity by this pathogen and the activation of effector-triggered immunity. Furthermore, we made a prospect on how to utilize the interaction mechanism between R. solanacearum and hosts to control the disease.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB11040600)the National Natural Science Foundation of China(31671278)the State Key Laboratory of Plant Genomics of China(2015B0129-02)
文摘Growing evidence indicates that actin cytoskeleton is involved in plant innate immune responses, but the functional mechanism remains largely unknown. Here, we investigated the behavior of a cotton profilin gene （GhPFN2） in response to Verticillium dahliae invasion, and evaluated its contribution to plant defense against this soil-borne fungal pathogen. GhPFN2 expression was up-regulated when cotton root was inoculated with V. dahliae, and the actin architecture was reorganized in the infected root cells, with a clear increase in the density of filamentous actin and the extent of actin btmdling. Compared to the wild type, GhPFN2-overexpressing cotton plants showed enhanced protection against V. dahliae infection and the actin cytoskeleton organization in root epidermal cells was clearly altered, which phenocopied that of the wild-type （WT） root cells challenged with V. dahliae. These results provide a solid line of evidence important for defense against V. dahliae infection. showing that actin cytoskeleton reorganization involving GhPFN2 is
