Patch clamp techniques were employed to investigate if calcium dependent protein kinases (CDPKs) be involved in the signal transduction pathways of stomatal movement regulation by the phytohormone abscisic acid (ABA...Patch clamp techniques were employed to investigate if calcium dependent protein kinases (CDPKs) be involved in the signal transduction pathways of stomatal movement regulation by the phytohormone abscisic acid (ABA) in Vicia faba. Stomatal opening was completely inhibited by external application of 1 μmol/L ABA, and such ABA inhibition was significantly reversed by the addition of CDPK inhibitor trifluoperazine (TFP). The inward whole cell K + currents were inhibited by 60% in the presence of 1 μmol/L intracellular ABA, and this inhibition was completely abolished by the addition of CDPK competitive substrate histone Ⅲ S. The results suggest that CDPKs may be involved in the signal transduction cascades of ABA regulated stomatal movements.展开更多
The incidence and mortality of cancer have been increasing alarmingly in China and worldwide.Unlike other chronic diseases such as metabolic,cardiovascular,and neuropathies,most cancers,especially metastatic ones,are ...The incidence and mortality of cancer have been increasing alarmingly in China and worldwide.Unlike other chronic diseases such as metabolic,cardiovascular,and neuropathies,most cancers,especially metastatic ones,are still essentially uncontrollable in spite of significant progresses such as early diagnosis and immunotherapies.展开更多
文摘Patch clamp techniques were employed to investigate if calcium dependent protein kinases (CDPKs) be involved in the signal transduction pathways of stomatal movement regulation by the phytohormone abscisic acid (ABA) in Vicia faba. Stomatal opening was completely inhibited by external application of 1 μmol/L ABA, and such ABA inhibition was significantly reversed by the addition of CDPK inhibitor trifluoperazine (TFP). The inward whole cell K + currents were inhibited by 60% in the presence of 1 μmol/L intracellular ABA, and this inhibition was completely abolished by the addition of CDPK competitive substrate histone Ⅲ S. The results suggest that CDPKs may be involved in the signal transduction cascades of ABA regulated stomatal movements.
文摘The incidence and mortality of cancer have been increasing alarmingly in China and worldwide.Unlike other chronic diseases such as metabolic,cardiovascular,and neuropathies,most cancers,especially metastatic ones,are still essentially uncontrollable in spite of significant progresses such as early diagnosis and immunotherapies.