The plant aerial parts of three species, Urtica dioica L., Viola odorata L. and Melissa officinalis L. were collected at randomly-different locations, according to altitudes in May 2010. The aerial parts of Urtica wer...The plant aerial parts of three species, Urtica dioica L., Viola odorata L. and Melissa officinalis L. were collected at randomly-different locations, according to altitudes in May 2010. The aerial parts of Urtica were collected within three replications from different locations： Biare 1,090 masl （meters above sea level）, Tawile 1,450 masl and Awiser 1,680 masl. The aerial parts of Viola and Melissa were collected randomly within three replications at different locations： Biare 1,090 masl, Degashikhan 1,250 masl and Tawile 1,450 masl. The extracts of the aerial parts of these species were purified by filtrations for several times in preparation for HPLC analyses. The chromatograms of Urtica indicated the presence of five major important alkaloid components （fragrine, benzylisoquinoline, scopoletin, glucoquinone and dotriacotaine） and ten major important phenolic compounds （formic acid, tannin, chlorogenic acid, caffeoylmalic acid, anthocyanine, quercetin, zeaxanthin, luetin epoxide, coumarine and vanillin）. All the concentrations of alkaloid and phenolic compounds were increased significantly due to higher altitudes, except that of alkaloid dotriacotaine. The chromatograms of Viola indicated the presence of four major important alkaloid components （violine, isoquinoline, cycloviolacin and luteolin-3-glucoronide） and ten major important phenolic compounds （formic acid, tannin, chlorogenic acid, caffeoylmalic acid, anthocyanine, quercetin, zeaxanthin, luetin epoxide, coumarine and vanillin）. The results of the influence of altitudes showed that the concentrations of all alkaloids and phenolic compounds were increased significantly due to higher altitudes, except that of the alkaloid luteolin-3-glucoronide and the phenolic compounds zeaxanthin and luetin epoxide. The chromatograms of Melissa indicated the presence of five major essential oils （pinene, linalool, citronellol, geraniol and rosmarinic acid）. Their quantitative evaluations were influenced by altitudes indicating that the concentrations of all oils were increased significantly due to the higher altitude, except that of the pinene.展开更多
Objective: To determine any relationship between temperaments of medicinal plants referred to traditional Iranian manuscripts and their major chemical compounds. Methods: Plants used in traditional Iranian medicine we...Objective: To determine any relationship between temperaments of medicinal plants referred to traditional Iranian manuscripts and their major chemical compounds. Methods: Plants used in traditional Iranian medicine were categorized based on their major chemical compounds including alkaloids, phenolic compounds, and essential oils. Their temperaments were extracted from traditional herbal pharmacopeias of Iran. The possible relationship between major chemical compounds and temperaments of each group were evaluated. Results: Plants containing phenolic compounds as their major constituents are hot and dry temperaments except those contain tannins with cold and dry temperaments. Plants containing essential oils have hot and dry temperaments except those whose major essential oils with alcoholic structure which have cold and dry temperaments. Alkaloid-containing plants have cold and dry or hot and dry temperaments based on their alkaloidal structures. Conclusions: There is a close relationship between major chemical compounds of medicinal plants and their temperaments mentioned in traditional Iranian manuscripts.展开更多
Lichens are unique individuals which have been widely used in traditional medicines. This study was focused on the bioassayguided phytochemical investigation, and bioactivity evaluation on a lichens species, Parmotrem...Lichens are unique individuals which have been widely used in traditional medicines. This study was focused on the bioassayguided phytochemical investigation, and bioactivity evaluation on a lichens species, Parmotrema cooperi. This first bioassaydirected chemical study on P. cooperi has led to the isolation of ethyl heamatomate （1）, atraric acid （2）, ethyl orsellinate （3）, orsellinic acid （4）, lecanoric acid （5）, gyrophoric acid （6）, and licanorin （7）. The structures of 1-7 were mainly elucidated from spectroscopic methods including 1D, and 2D NMR spectroscopy, and mass spectrometry. These compounds were evaluated for their antiglycation, urease, a-chymotrypsin, and β-glucoronidase inhibitory activities. Few of the phenolic compounds showed significant, while most of them showed good inhibition of protein glycation, and urease activities.展开更多
Sesquiterpenoids are a class of 15-carbon secondary metabolites that play diverse roles in plant adaptation to environment. Cotton plants accumulate a large amount of sesquiterpene aldehydes （including gossypol） as ...Sesquiterpenoids are a class of 15-carbon secondary metabolites that play diverse roles in plant adaptation to environment. Cotton plants accumulate a large amount of sesquiterpene aldehydes （including gossypol） as phytoalexins against pathogens and herbivores. They are stored in pigment glands of aerial organs and in epidermal layers of roots. Several enzymes of goss- ypol biosynthesis pathway have been characterized, including 3-hydroxy-3-methylglutaryl coenzyme A reductase （HMGR） and farnesyl diphosphate synthase （FPS） that catalyze the formation of the precursor farnesyl diphosphate （FPP）, （＋）-6-cadinene synthase （CDN） which is the first enzyme committed to gossypol biosynthesis, and the downstream enzymes of CYP706B 1 and methyltransferase. Expressions of these genes are tightly regulated during cotton plants development and in- duced by jasmonate and fungi elicitors. The transcription factor GaWRKY1 has been shown to be involved in gossypol path- way regulation. Recent development of new genomic platforms and methods and releases of diploid and tetraploid cotton ge- nome sequences will greatly facilitate the elucidation of gossypol biosynthetic pathway and its regulation.展开更多
文摘The plant aerial parts of three species, Urtica dioica L., Viola odorata L. and Melissa officinalis L. were collected at randomly-different locations, according to altitudes in May 2010. The aerial parts of Urtica were collected within three replications from different locations： Biare 1,090 masl （meters above sea level）, Tawile 1,450 masl and Awiser 1,680 masl. The aerial parts of Viola and Melissa were collected randomly within three replications at different locations： Biare 1,090 masl, Degashikhan 1,250 masl and Tawile 1,450 masl. The extracts of the aerial parts of these species were purified by filtrations for several times in preparation for HPLC analyses. The chromatograms of Urtica indicated the presence of five major important alkaloid components （fragrine, benzylisoquinoline, scopoletin, glucoquinone and dotriacotaine） and ten major important phenolic compounds （formic acid, tannin, chlorogenic acid, caffeoylmalic acid, anthocyanine, quercetin, zeaxanthin, luetin epoxide, coumarine and vanillin）. All the concentrations of alkaloid and phenolic compounds were increased significantly due to higher altitudes, except that of alkaloid dotriacotaine. The chromatograms of Viola indicated the presence of four major important alkaloid components （violine, isoquinoline, cycloviolacin and luteolin-3-glucoronide） and ten major important phenolic compounds （formic acid, tannin, chlorogenic acid, caffeoylmalic acid, anthocyanine, quercetin, zeaxanthin, luetin epoxide, coumarine and vanillin）. The results of the influence of altitudes showed that the concentrations of all alkaloids and phenolic compounds were increased significantly due to higher altitudes, except that of the alkaloid luteolin-3-glucoronide and the phenolic compounds zeaxanthin and luetin epoxide. The chromatograms of Melissa indicated the presence of five major essential oils （pinene, linalool, citronellol, geraniol and rosmarinic acid）. Their quantitative evaluations were influenced by altitudes indicating that the concentrations of all oils were increased significantly due to the higher altitude, except that of the pinene.
文摘Objective: To determine any relationship between temperaments of medicinal plants referred to traditional Iranian manuscripts and their major chemical compounds. Methods: Plants used in traditional Iranian medicine were categorized based on their major chemical compounds including alkaloids, phenolic compounds, and essential oils. Their temperaments were extracted from traditional herbal pharmacopeias of Iran. The possible relationship between major chemical compounds and temperaments of each group were evaluated. Results: Plants containing phenolic compounds as their major constituents are hot and dry temperaments except those contain tannins with cold and dry temperaments. Plants containing essential oils have hot and dry temperaments except those whose major essential oils with alcoholic structure which have cold and dry temperaments. Alkaloid-containing plants have cold and dry or hot and dry temperaments based on their alkaloidal structures. Conclusions: There is a close relationship between major chemical compounds of medicinal plants and their temperaments mentioned in traditional Iranian manuscripts.
基金the financial support of the Higher Education Commission,Pakistan,through the project entitled"High Resolution X-Ray Analysis of Pharmaceutically Important Enzymes in Complex with Plant-based Inhibitions as basis for Rational Drug Design (20-1364/ R&D/09)"
文摘Lichens are unique individuals which have been widely used in traditional medicines. This study was focused on the bioassayguided phytochemical investigation, and bioactivity evaluation on a lichens species, Parmotrema cooperi. This first bioassaydirected chemical study on P. cooperi has led to the isolation of ethyl heamatomate （1）, atraric acid （2）, ethyl orsellinate （3）, orsellinic acid （4）, lecanoric acid （5）, gyrophoric acid （6）, and licanorin （7）. The structures of 1-7 were mainly elucidated from spectroscopic methods including 1D, and 2D NMR spectroscopy, and mass spectrometry. These compounds were evaluated for their antiglycation, urease, a-chymotrypsin, and β-glucoronidase inhibitory activities. Few of the phenolic compounds showed significant, while most of them showed good inhibition of protein glycation, and urease activities.
基金the State Key Basic Research Program of China (2013CB127000)the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB11030300)+1 种基金the National Natural Science Foundation of China (31300255)the Special Fund for Shanghai Landscaping Administration Bureau Program (G142425, F112418).
文摘Sesquiterpenoids are a class of 15-carbon secondary metabolites that play diverse roles in plant adaptation to environment. Cotton plants accumulate a large amount of sesquiterpene aldehydes （including gossypol） as phytoalexins against pathogens and herbivores. They are stored in pigment glands of aerial organs and in epidermal layers of roots. Several enzymes of goss- ypol biosynthesis pathway have been characterized, including 3-hydroxy-3-methylglutaryl coenzyme A reductase （HMGR） and farnesyl diphosphate synthase （FPS） that catalyze the formation of the precursor farnesyl diphosphate （FPP）, （＋）-6-cadinene synthase （CDN） which is the first enzyme committed to gossypol biosynthesis, and the downstream enzymes of CYP706B 1 and methyltransferase. Expressions of these genes are tightly regulated during cotton plants development and in- duced by jasmonate and fungi elicitors. The transcription factor GaWRKY1 has been shown to be involved in gossypol path- way regulation. Recent development of new genomic platforms and methods and releases of diploid and tetraploid cotton ge- nome sequences will greatly facilitate the elucidation of gossypol biosynthetic pathway and its regulation.
