AIMS Antirejection therapy with tripterygium wolfordii(TW) and low-dose cyclosporine(CsA)was better than treatments with large dosage CsA in small bowel transplantation. METHODS This paper presents the experiment of t...AIMS Antirejection therapy with tripterygium wolfordii(TW) and low-dose cyclosporine(CsA)was better than treatments with large dosage CsA in small bowel transplantation. METHODS This paper presents the experiment of two step segmental small bowel transplantation with TW,a traditional Chinese medicine and low dose CsA in pigs. RESULTS Rejection was developed in Group I without im- munosuppression as well as in Group Ⅳ treated with low-dose CsA.The mean survival time of grafts was 12.8±2.7days and 12.4±2.6 days respectively.The animals of Group Ⅱ were treated with high-dose CsA for 100 days and then with TW.in which two pigs were killed for severe pneumonia on day 92,97 and two pigs survived more than 348 and 327 days respective- ly.Five animals in Group Ⅲ in which TW and low-dose CsA were administered for 100 days and then TW was the only drug used in living animals,survived 243.2±90.9 days,none of which succumbed to infection. CONCLUSIONS:We are the first to use TW in small bowel transplantation and f Group Ⅰ(n=10):control group,received no immunosuppression.展开更多
Sericostaehys seandens is a monocarpic and heliophilous liana, native in tropical African forests. In the montane forests of the Kahuzi-Biega National Park （KBNP） （East of DR Congo）, it has been expanding very str...Sericostaehys seandens is a monocarpic and heliophilous liana, native in tropical African forests. In the montane forests of the Kahuzi-Biega National Park （KBNP） （East of DR Congo）, it has been expanding very strongly for a decade, and is currently considered as having negative impacts on biodiversity conservation. In this paper, we test if S. scandens differs from three co-occurring, native, non spreading lianas （Gouania longispicata, Tacazzea apiculata and Adenia bequaertii） for functional traits which might influence plant expansion. For leaf traits （SLA, dry matter content, nitrogen concentration）, S. scandens did not show extreme values compared to those of the three other lianas. In contrast, S. scandens had much higher biomass allocation to sexual reproduction. It also differs from the three other lianas for its reproductive strategy that combines both vegetative propagation and sexual reproduct/on, and propagule dispersal by wind. Moreover, S. scandens has larger leaves and a greater number of lateral branches per unit stem length. It is argued that the particular combination of functional traits exhibited by S.scandens may in part explain its propensity to behave as an opportunistic weed in the disturbed areas in the montane forests of Kahuzi-Biega.展开更多
The purpose of this investigation is to demonstrate whether triptolide can inhibit TNF-α selectively and to study the mechanism. The ability of triptolide to inhibit the production of TNF-α and IFN-γ stimulated by ...The purpose of this investigation is to demonstrate whether triptolide can inhibit TNF-α selectively and to study the mechanism. The ability of triptolide to inhibit the production of TNF-α and IFN-γ stimulated by lipopolysaccharide (LPS) and phytohemagglutinin (PHA) on peripheral blood mononuclear cell (PBMC) from healthy donors was measured by ELISA assays. The immunological mechanism of action of triptolide on TNF-α was investigated by pre-treatment with triptolide of PBMC and monocytes followed by analysis with Flow Cytometry (FCM). The inhibition of TNF-α and IFN-γ by triptolide occurred in a dose dependent manner and the IC 50 was equal to 5-10 ng/ml for TNF-α and 0.1-1 ng/ml for IFN-γ. The concentrations of TNF-α measured after the different pre-treatments with triptolide on PBMC and monocytes are consistent with its effects on a population of CD14 +/TNF-α + monocytes shown on FCM. The two methods of pre-treatments with triptolide may suggest different clinical significances. Immunophenotyping analysis with FCM revealed that triptolide may compete with LPS for binding to the CD14 receptor. The study provides basic evidence that triptolide may be used as an anti-inflammatory reagent for treatment of leprosy reactions.展开更多
文摘AIMS Antirejection therapy with tripterygium wolfordii(TW) and low-dose cyclosporine(CsA)was better than treatments with large dosage CsA in small bowel transplantation. METHODS This paper presents the experiment of two step segmental small bowel transplantation with TW,a traditional Chinese medicine and low dose CsA in pigs. RESULTS Rejection was developed in Group I without im- munosuppression as well as in Group Ⅳ treated with low-dose CsA.The mean survival time of grafts was 12.8±2.7days and 12.4±2.6 days respectively.The animals of Group Ⅱ were treated with high-dose CsA for 100 days and then with TW.in which two pigs were killed for severe pneumonia on day 92,97 and two pigs survived more than 348 and 327 days respective- ly.Five animals in Group Ⅲ in which TW and low-dose CsA were administered for 100 days and then TW was the only drug used in living animals,survived 243.2±90.9 days,none of which succumbed to infection. CONCLUSIONS:We are the first to use TW in small bowel transplantation and f Group Ⅰ(n=10):control group,received no immunosuppression.
基金the Belgian Development Cooperation for the financial help
文摘Sericostaehys seandens is a monocarpic and heliophilous liana, native in tropical African forests. In the montane forests of the Kahuzi-Biega National Park （KBNP） （East of DR Congo）, it has been expanding very strongly for a decade, and is currently considered as having negative impacts on biodiversity conservation. In this paper, we test if S. scandens differs from three co-occurring, native, non spreading lianas （Gouania longispicata, Tacazzea apiculata and Adenia bequaertii） for functional traits which might influence plant expansion. For leaf traits （SLA, dry matter content, nitrogen concentration）, S. scandens did not show extreme values compared to those of the three other lianas. In contrast, S. scandens had much higher biomass allocation to sexual reproduction. It also differs from the three other lianas for its reproductive strategy that combines both vegetative propagation and sexual reproduct/on, and propagule dispersal by wind. Moreover, S. scandens has larger leaves and a greater number of lateral branches per unit stem length. It is argued that the particular combination of functional traits exhibited by S.scandens may in part explain its propensity to behave as an opportunistic weed in the disturbed areas in the montane forests of Kahuzi-Biega.
文摘The purpose of this investigation is to demonstrate whether triptolide can inhibit TNF-α selectively and to study the mechanism. The ability of triptolide to inhibit the production of TNF-α and IFN-γ stimulated by lipopolysaccharide (LPS) and phytohemagglutinin (PHA) on peripheral blood mononuclear cell (PBMC) from healthy donors was measured by ELISA assays. The immunological mechanism of action of triptolide on TNF-α was investigated by pre-treatment with triptolide of PBMC and monocytes followed by analysis with Flow Cytometry (FCM). The inhibition of TNF-α and IFN-γ by triptolide occurred in a dose dependent manner and the IC 50 was equal to 5-10 ng/ml for TNF-α and 0.1-1 ng/ml for IFN-γ. The concentrations of TNF-α measured after the different pre-treatments with triptolide on PBMC and monocytes are consistent with its effects on a population of CD14 +/TNF-α + monocytes shown on FCM. The two methods of pre-treatments with triptolide may suggest different clinical significances. Immunophenotyping analysis with FCM revealed that triptolide may compete with LPS for binding to the CD14 receptor. The study provides basic evidence that triptolide may be used as an anti-inflammatory reagent for treatment of leprosy reactions.
