多排螺旋CT对腰椎椎小关节退行性变影像诊断意义分析

为了促进腰椎椎小关节退行性变的影像学诊断,研究以2021年06月-2022年06月收治的40例腰背痛及腰腿痛症状者为对象,实施X线与CT检查,观察不同方法进行检查的确诊率等指标情况。研究结果表明,使用CT、X线片检查腰椎骨质增生检出率分别为67.50%、57.50%;CT扫描椎间盘突出检出率为52.50%,X线片检查的检出率为12.50%;CT扫描椎管狭窄检出率为50.00%,X线片检查的检出率为12.50%;CT、X线片检查椎小关节增生检出率依次为72.50%、25.00%。可知,X线片检查可以看到脊柱腰段序列病变情况,腰椎小关节退行性变作为腰椎退行性变疾病,多排螺旋CT扫描能够更清晰的展现腰椎退行性变情况,诊断准确性较高。

^(99)mTc-MDP全身骨显像及同机断层融合骨显像在颈椎疾病诊断中的应用价值研究

目的:探讨^(99)mTc-MDP全身骨显像与同机断层融合骨显像对颈椎病变的诊断。方法:回顾性分析我院于2018年6月—2019年6月收治的54例颈椎病变患者作为研究对象,其中颈椎退行性变患者19例,颈椎转移性病变患者35例。两组均行^(99)mTc-MDP全身骨显像与同机断层融合骨显像,比较析^(99)mTc-MDP全身骨显像与同机断层融合骨显像的诊断效能。结果:^(99)mTc-MDP全身骨显像共检出36例,其中颈椎退行性病变13例,颈椎转移性病变23例。同机断层融合骨显像共检出53例,其中颈椎退行性病变18例,颈椎转移性病变35例。同机断层融合骨显像诊断的灵敏度、特异性、准确性、阳性预测值、阴性预测值均明显高于^(99)mTc-MDP全身骨显像(P<0.05)。结论—同机断层融合骨显像对颈椎病变患者的检出率高于^(99)mTc-MDP全身骨显像,同机断层融合骨显像有助于鉴别诊断颈椎良恶性病变,具有临床应用价值。

CLINICAL ANALYSIS ON TREATMENT OF LUMBAR VERTEBRAL RETROGRADE AFFECTION WITH ACUPUNCTURE,TDP-IRRADIATION AND CUPPING

In the present paper, 70 cases of retrograde affection of the lumbar vertebrae are randomly and evenly divided into treatment group and control group. Shenshu (BL 23), Qihaishu (BL 24), Dachangshu (BL 25), Weizhong (BL 40), Jiaji (EX B 2), etc. are used. In treatment group, acupuncture, TDP irradiation and cupping are performed, and in control group, only acupuncture is given. After 30 sessions (3 therapeutic courses) of treatment, in treatment and control groups, the total effective rates are 91.43% and 71.43% respectively. The therapeutic effect of comprehensive treatment is significantly superior to that of simple acupuncture therapy (P<0.05).

Adenovirus-mediated GDF-5 promotes the extracellular matrix expression in degenerative nucleus pulposus cells

Objective： To construct a recombinant adenovirus vector-carrying human growth and differentiation factor-5 （GDF-5） gene, investigate the biological effects of adenovirus-mediated GDF-5 （Ad-GDF-5） on extracellular matrix （ECM） expression in human degenerative disc nucleus pulposus （NP） cells, and explore a candidate gene therapy method for intervertebral disc degeneration （IDD）. Methods： Human NP cells of a degenerative disc were isolated, cultured, and infected with Ad-GDF-5 using the AdEasy-1 adenovirus vector system. On Days 3, 7, 14, and 21, the contents of the sulfated glycosaminoglycan （sGAG）, deoxyribonucleic acid （DNA） and hydroxyproline （Hyp）, synthesis of proteoglycan and collagen II, gene expression of collagen II and aggrecan, and NP cell proliferation were assessed. Results： The adenovirus was an effective vehicle for gene delivery with prolonged expression of GDF-5. Biochemical analysis revealed increased sGAG and Hyp contents in human NP cells infected by Ad-GDF-5 whereas there was no conspicuous change in basal medium （BM） or Ad-green fluorescent protein （GFP） groups. Only cells in the Ad-GDF-5 group promoted the production of ECM, as demonstrated by the secretion of proteoglycan and up-regulation of collagen II and aggrecan at both protein and mRNA levels. The NP cell proliferation was significantly promoted. Conclusions： The data suggest that Ad-GDF-5 gene therapy is a potential treatment for IDD, which restores the functions of degenerative intervertebral disc through enhancing the ECM production of human NP ceils.

Acupuncture-moxibustion at Jiaji(EX-B 2) points for intervertebral disc herniation: a systematic review

By organizing and classifying the literatures related to acupuncture-moxibustion at Jiaji （EX-B 2） points in treating intervertebral disc herniation （IDH） published before December 32, 2013, to summarize the application status and features of Jiaji （EX-B 2） points. It＇s found that its most favorable indication is IDH. It also further analyzed the action mechanism of acupuncture-moxibustion at Jiaji （EX-B 2） points in treating IDH, and brought up some ideas to highlight the further study.

Gene expression of collagen types IX and X in the lumbar disc

Objective:To study gene expression of collagen types IX and X in human lumbar intervertebral discs during aging and degeneratio n and to explore the role of collagen types IX and X in disc degeneration. Methods:Fetal, adult and pathologic specimens were subjected t o in situ hybridization with cDNA probes to investigate mRNA-expressions of typ es IX and X collagen gene. Results:In fetal intervertebral discs, positive mRNA hybridiza tion signals of type IX collagen were concentrated in the nucleus pulposus and t he inner layer of anulus fibrosus. Interstitial matrix of the nucleus pulposus a lso showed positive type X collagen staining. Positive mRNA hybridization signal s of types IX and X were not detected in the middle and outer layers of anulus f ibrosus. In adult specimens, expression of type IX collagen mRNA was markedly de creased. No hybridization signals of type X collagen was observed. As for pathol ogical specimens, there was no gene expression of type IX collagen. In severe de generated discs from adults, there were focal positive expressions of type X col lagen. Conclusions:Obvious changes of collagen gene expression occur with aging. Expression of type IX collagen decreases in adult and pathological d iscs. Results of type X collagen expression suggest that type X collagen is expr essed only in older adult and senile discs (i.e., when disc degeneration has alr eady reached a terminal stage), indicating the terminal stage of degeneration.