OBJECTIVE:To explore the effect of scraping therapy on the Interleukin-1 (IL-1) levels of rats with lumbar disc herniation (LDH). METHODS: Fifty male rats were devided into a blank group (A), a sham operation group (B...OBJECTIVE:To explore the effect of scraping therapy on the Interleukin-1 (IL-1) levels of rats with lumbar disc herniation (LDH). METHODS: Fifty male rats were devided into a blank group (A), a sham operation group (B), a model group (C), a scraping group (D), and a drug group (E). The rats in the group B were treated with sham operation, and groups C, D and E were made into the LDH model by operation. After operation group C were treated with no interventions, D were given scraping and E were fed with azathioprine Then the IL-1 levels of different groups were detected by enzyme-linked immuno sorbent assay method. And the transplanted coccygeal vertebra discs were observed by pathological section. RESULTS: The IL-1 levels in the groups C, D, and E were significantly higher than those in the groups A and B (all P<0.01), which proved the operationwas successful.The IL-1 levels in the groups D and E at different periods had statistical significance (F= 414.158, P<0.01). The treatment periods and interventions have interation (F=46.613, P<0.01). Multiple comparison results showed that the IL-1 levels in the groups D and E was significantly lower than that in the group C (P<0.01), while the IL-1 levels between the groups D and E had no statistical significance (P>0.05). Moreover, pathological section indicated that immuno-inflammatory response was hardly found in coccygeal vertebra discs in the groups A and B, while local immuno-inflammatory responses of the groups D and E were much lighter than that of the group C. CONCLUSION: Scraping therapy could inhibit the immuno-inflammatory responses in the rats with LDH caused by transplantation of autologous nu cleus pulposus.展开更多
OBJECTIVE:The aims of this study were to determine the anti-inflammatory and analgesic effects of Yaotuitong(translation:low back and leg pain) capsules,a Chinese herbal preparation,and the histological changes it ind...OBJECTIVE:The aims of this study were to determine the anti-inflammatory and analgesic effects of Yaotuitong(translation:low back and leg pain) capsules,a Chinese herbal preparation,and the histological changes it induces in experimental rats with chemically induced radicular neuritis.METHODS:Wistar rats were randomly divided into normal,model,Western medicine,and traditional Chinese medicine groups(n=24 per group).We surgically duplicated a chemical radicular neuritis model to simulate lumbar intervertebral disc protrusion.Granuloma formation was measured on postoperative days(PODs) 3,7,14,and 21.Prostaglandin E 2 and 5-hydroxytryptamine(inflammation mediators) levels in the surrounding tissue and the histology of the nerve root were determined on PODs 7 and 14.RESULTS:Yaotuitong capsules significantly reduced prostaglandin E 2(P<0.01) and 5-hydroxytryptamine(P<0.01)levels in tissue surrounding the nerve root.It also inhibited granuloma formation(P<0.05).CONCLUSION:Yaotuitong capsules have anti-in-flammatory and analgesic effects that can alleviate the discomfort of lumbar intervertebral disc protrusion.展开更多
基金Supported by Natural Science Foundation of University in Jiangsu Province (No. 11KJB360003)
文摘OBJECTIVE:To explore the effect of scraping therapy on the Interleukin-1 (IL-1) levels of rats with lumbar disc herniation (LDH). METHODS: Fifty male rats were devided into a blank group (A), a sham operation group (B), a model group (C), a scraping group (D), and a drug group (E). The rats in the group B were treated with sham operation, and groups C, D and E were made into the LDH model by operation. After operation group C were treated with no interventions, D were given scraping and E were fed with azathioprine Then the IL-1 levels of different groups were detected by enzyme-linked immuno sorbent assay method. And the transplanted coccygeal vertebra discs were observed by pathological section. RESULTS: The IL-1 levels in the groups C, D, and E were significantly higher than those in the groups A and B (all P<0.01), which proved the operationwas successful.The IL-1 levels in the groups D and E at different periods had statistical significance (F= 414.158, P<0.01). The treatment periods and interventions have interation (F=46.613, P<0.01). Multiple comparison results showed that the IL-1 levels in the groups D and E was significantly lower than that in the group C (P<0.01), while the IL-1 levels between the groups D and E had no statistical significance (P>0.05). Moreover, pathological section indicated that immuno-inflammatory response was hardly found in coccygeal vertebra discs in the groups A and B, while local immuno-inflammatory responses of the groups D and E were much lighter than that of the group C. CONCLUSION: Scraping therapy could inhibit the immuno-inflammatory responses in the rats with LDH caused by transplantation of autologous nu cleus pulposus.
文摘OBJECTIVE:The aims of this study were to determine the anti-inflammatory and analgesic effects of Yaotuitong(translation:low back and leg pain) capsules,a Chinese herbal preparation,and the histological changes it induces in experimental rats with chemically induced radicular neuritis.METHODS:Wistar rats were randomly divided into normal,model,Western medicine,and traditional Chinese medicine groups(n=24 per group).We surgically duplicated a chemical radicular neuritis model to simulate lumbar intervertebral disc protrusion.Granuloma formation was measured on postoperative days(PODs) 3,7,14,and 21.Prostaglandin E 2 and 5-hydroxytryptamine(inflammation mediators) levels in the surrounding tissue and the histology of the nerve root were determined on PODs 7 and 14.RESULTS:Yaotuitong capsules significantly reduced prostaglandin E 2(P<0.01) and 5-hydroxytryptamine(P<0.01)levels in tissue surrounding the nerve root.It also inhibited granuloma formation(P<0.05).CONCLUSION:Yaotuitong capsules have anti-in-flammatory and analgesic effects that can alleviate the discomfort of lumbar intervertebral disc protrusion.
