槐树种子的化学成分研究

AIM Isolation and structural elucidation of the constituents from seeds of Sophora japonica L. METHODS Solvent extraction and column chromatography were used. IR, MS, 1HNMR and 13 CNMR spectra were applied for structural elucidation. RESULTS Eight compounds were isolated and identified as genistein(1), sophoraricoside (2), sissotrin(3), sophorabioside(4), kaempferol-7-O-α-L-rhamnoside(5), rutin(6), isoscutellarein(7), and gallic acid(8). CONCLUSION Among them, compounds 3,5,7 and 8 were isolated from this plant for the first time.

一测多评法测定槐角中异黄酮类成分的含量

目的:采用一测多评,建立槐角中异黄酮类成分的含量测定方法。方法:采用Agilent Extend C18色谱柱(250 mm×4.6mm,5μm),以甲醇-乙腈-0.1%磷酸水溶液为流动相,流速为1.0 mL·min-1,柱温为30℃,检测波长为260 nm,建立槐角苷与槐属双苷、染料木苷和染料木素的相对校正因子,并以相对保留时间作为目标峰的定位标准。结果:色谱峰分离度良好,槐角苷在0.374～7.49μg、槐属双苷在2.16×10-2～0.432μg、染料木苷在2.61×10-2～0.522μg、染料木素在1.02×10-2～0.203μg呈良好线性关系,平均加样回收率槐角苷为100.7%、槐属双苷为96.3%、染料木苷为98.6%、染料木素为100.8%。槐角苷对槐属双苷、染料木苷和染料木素的相对校正因子分别为0.80、1.03、1.61。相对保留时间分别为0.42、0.81、1.26。结论:采用一测多评法测定槐角中异黄酮类成分的含量是准确、可行的。