目的建立液相色谱-串联质谱法同时测定地榆槐角丸中芦丁、槐角苷、柚皮苷、染料木苷、黄芩苷、染料木素、芦荟大黄素、大黄酸和大黄素含量的方法。方法采用C18色谱柱,乙腈-0.1%乙酸水为流动相,梯度洗脱。采用电喷雾离子源,监测模式为负...目的建立液相色谱-串联质谱法同时测定地榆槐角丸中芦丁、槐角苷、柚皮苷、染料木苷、黄芩苷、染料木素、芦荟大黄素、大黄酸和大黄素含量的方法。方法采用C18色谱柱,乙腈-0.1%乙酸水为流动相,梯度洗脱。采用电喷雾离子源,监测模式为负离子,定量检测采用多重反应离子模式。结果在15min内地榆槐角丸中9种有效成分在所测定的浓度范围内线性关系均良好,加样回收率(n=3)分别为96.2%、97.5%、98.3%、99.6%、97.5%、100.1%、94.2%、98.7%和99.7%,相对标准偏差(relative standard deviation,RSD)值分别为1.3%、1.2%、1.4%、1.7%、0.5%、0.54%、0.9%、1.0%和0.82%。结论该方法简便、准确、重现性好、专属性高,可用于地榆槐角丸的质量控制。展开更多
Objective:To determine the contents of the four efficacious elements(quercetin;ellagic acid) in Huaijiao Wan by RP-HPLC.Method:High Performance Liquid Chromatograph is employed with a Column of Diamonsil ODS-C18(4.6mm...Objective:To determine the contents of the four efficacious elements(quercetin;ellagic acid) in Huaijiao Wan by RP-HPLC.Method:High Performance Liquid Chromatograph is employed with a Column of Diamonsil ODS-C18(4.6mm×250mm,5.0μm) and a mobile phase of MeOH-H2O-HAc(2∶3∶0.15),the detection wavelength being 270 nm and the column temperature being 300C.Result:Nice linear relation exists when,quercetin is within 0.2048-1.229μg and ellagic acid within 0.5325-3.192 μg.The R value of each group is 0.9991 and 0.9998.The average rate of recovery of the two groups is between 98.0% and 99.1%.The RSD of each group is 1.02% and 0.40%(n=5).Conclusion:With this method,the two efficacious elements could be determined simultaneously.And for its convenience,nicety,high sensitivity and fidelity,it is applicable to the quality examination of Huaijiao products and to manufacturers’ control of quality.展开更多
文摘目的建立液相色谱-串联质谱法同时测定地榆槐角丸中芦丁、槐角苷、柚皮苷、染料木苷、黄芩苷、染料木素、芦荟大黄素、大黄酸和大黄素含量的方法。方法采用C18色谱柱,乙腈-0.1%乙酸水为流动相,梯度洗脱。采用电喷雾离子源,监测模式为负离子,定量检测采用多重反应离子模式。结果在15min内地榆槐角丸中9种有效成分在所测定的浓度范围内线性关系均良好,加样回收率(n=3)分别为96.2%、97.5%、98.3%、99.6%、97.5%、100.1%、94.2%、98.7%和99.7%,相对标准偏差(relative standard deviation,RSD)值分别为1.3%、1.2%、1.4%、1.7%、0.5%、0.54%、0.9%、1.0%和0.82%。结论该方法简便、准确、重现性好、专属性高,可用于地榆槐角丸的质量控制。
文摘Objective:To determine the contents of the four efficacious elements(quercetin;ellagic acid) in Huaijiao Wan by RP-HPLC.Method:High Performance Liquid Chromatograph is employed with a Column of Diamonsil ODS-C18(4.6mm×250mm,5.0μm) and a mobile phase of MeOH-H2O-HAc(2∶3∶0.15),the detection wavelength being 270 nm and the column temperature being 300C.Result:Nice linear relation exists when,quercetin is within 0.2048-1.229μg and ellagic acid within 0.5325-3.192 μg.The R value of each group is 0.9991 and 0.9998.The average rate of recovery of the two groups is between 98.0% and 99.1%.The RSD of each group is 1.02% and 0.40%(n=5).Conclusion:With this method,the two efficacious elements could be determined simultaneously.And for its convenience,nicety,high sensitivity and fidelity,it is applicable to the quality examination of Huaijiao products and to manufacturers’ control of quality.
