民间文学艺术表达及其权利界定

民间文学艺术表达不同于民间文学艺术作品和民间文艺衍生作品。民间文学艺术表达的实质是具有传统艺术特色的特定模式化信息。根据传播范围,民间文学艺术表达的权利归属体系确定为传承人和群体、群体及国家三个层次。

Expression patterns of transforming growth factor-beta and its receptors in gastric mucosa of patients with refractory gastric ulcer

AIM: Transforming growth factor-β (TGF-β) plays a regulatory role in tissue repair. In a previous study, we found that TGF-β and its receptors were expressed in gastric mucosa of patients with well-healed gastric ulcers, as demonstrated by immunohistochemistry. To further characterize the role of TGF-β and its receptors in repairing gastric ulcers, we investigated the expression patterns of TGF-β and its receptors in gastric mucosa by in situ hybridization and reverse transcriptase-polymerase chain reaction (RT-PCR).METHODS: Seventy-four patients with endoscopically proven gastric ulcers were eligible for participation in this study. All patients had routine biopsies on initial endoscopy and were then treated for 12 wk with an H2 blocker. Repeat endoscopy was then performed. There were 8 patients with poorly healed ulcers, and biopsies were taken from the margin of the residual ulcers. These tissue samples, along with biopsy of gastric mucosa near the original ulcers from 8 randomly selected patients with well-healed ulcers were examined for TGF-β and TGF-β receptor Ⅱ mRNA by RT-PCR and in situ hybridization, as well as immunohistochemistry.RESULTS: TGF-β and TGF-β receptor Ⅱ were strongly expressed in tissues from patients with well-healed ulcers.Four of the 8 patients with poor healing had low or absent expression of TGF-β or TGF-β receptor Ⅱ mRNA. All cases positive by RT-PCR assay were confirmed by in situ hybridization as well as immunohistochemistry.CONCLUSION: It is suggested that TGF-β and its receptors are important for gastric ulcer healing. These results may have implications for further investigation of the healing process and in predicting response to therapy.

Developmental expression amphioxus Branchiostoma pattern of calmodulin gene in belcheri tsingtauense

We investigated the developmental expression pattern of AmphiCaM in cephalochordate amphioxus （Branchiostoma belcheri tsingtauense）. We cultured and sampled the animals at different developmental stages （eggs and larvae）, and used in-situ hybridization and northern blotting to document the spatial and temporal changes in AmphiCaM expression. The alimentary tract dominates the development from the late neurula stage to the adult stage. AmphiCaM expression increased significantly in the alimentary tract during the late neurula stage and remained elevated in the adults. Our results indicate that AmphiCaM is involved in the differentiation of the alimentary tract in amphioxus; and furthermore, provide an insight into the change in function of CaM genes during evolution.

Expression Pattern of Peptide and Amino Acid Genes in Digestive Tract of Transporter Juvenile Turbot(Scophthalmus maximus L.)

Turbot(Scophthalmus maximus L.),a carnivorous fish species with high dietary protein requirement,was chosen to examine the expression pattern of peptide and amino acid transporter genes along its digestive tract which was divided into six segments including stomach,pyloric caeca,rectum,and three equal parts of the remainder of the intestine.The results showed that the expression of two peptide and eleven amino acid transporters genes exhibited distinct patterns.Peptide transporter 1(Pep T1) was rich in proximal intestine while peptide transporter 2(PepT2) was abundant in distal intestine.A number of neutral and cationic amino acid transporters expressed richly in whole intestine including B^0-type amino acid transporter 1(B^0AT1),L-type amino acid transporter 2(LAT2),T-type amino acid transporter 1(TAT1),proton-coupled amino acid transporter 1(PAT1),y^+L-type amino acid transporter 1(y^+LAT1),and cationic amino acid transporter 2(CAT2) while ASC amino acid transporter 2(ASCT2),sodium-coupled neutral amino acid transporter 2(SNAT2),and y^+L-type amino acid transporter 2(y^+LAT2) abundantly expressed in stomach.In addition,system b^(0,+) transporters(rBAT and b^(0,+)AT) existed richly in distal intestine.These findings comprehensively characterized the distribution of solute carrier family proteins,which revealed the relative importance of peptide and amino acid absorption through luminal membrane.Our findings are helpful to understand the mechanism of the utilization of dietary protein in fish with a short digestive tract.

「前置き表現」的语言策略及文化意义

「突然ですが、皆さんは中国料理が好きですか」中的「突然ですが」这类表述可视为一种典型的「前置き表現」,而「前置き表現」的大量使用也是以日语为母语者语言方式的一大特征。本文将「前置き表現」的使用视为一种与文化因素相关的语言行为,从"语言策略"及"文化意义"的角度对「前置き表現」做一个基本的透视,具体指出特定的社会行为规范和文化价值取向等对「前置き表現」的语言策略等产生的几种主要影响。

A genome-wide analysis of the ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES(AS2/LOB) gene family in barley(Hordeum vulgare L.)

ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES（AS2/LOB） genes are a family of plant specific transcription factors, which play an important role in the regulation of plant lateral organ development and metabolism. However, a genome-wide analysis of the AS2/LOB gene family is still not available for barley. In the present study, 24 AS2-like（ASL）/LOB domain（LBD） genes were identified based on the barley（Hordeum vulgare L.） genome sequence. A phylogenetic tree of ASL/LBD proteins from barley, Arabidopsis, maize, and rice was constructed. The ASL/LBD genes were classified into two classes, class I and class II, which were divided into five and two subgroups, respectively. Genes homologous in barley and Arabidopsis were analyzed. In addition, the structure and chromosomal locations of the genes were analyzed. Expression profiles indicated that barley HvA SL/LBD genes exhibit a variety of expression patterns, suggesting that they are involved in various aspects of physiological and developmental processes. This genome-wide analysis of the barley AS2/LOB gene family contributes to our understanding of the functions of the AS2/LOB gene family.

Theoretical analysis of the harmonic characteristics of modular multilevel converters

To understand the operation principle of the modular multilevel converter(MMC)deeply,it is necessary to study the harmonic characteristics of the MMC theoretically.Besides,the analytical harmonic formulas of the MMC are useful in designing the main circuit,reducing the losses and improving the waveform quality.Based on the average switching function and the Fourier series harmonic analysis,this paper deduces the analytical expressions for such electrical quantities as the arm voltage,the arm current,the capacitor voltage,the capacitor current and the circulating current of the MMC.Finally,a digital model of a 21-level MMC-HVDC system is realized in PSCAD/EMTDC.The results of the analytical expressions coincide with the simulation results,which verify the effectiveness and feasibility of the proposed analytical expressions.