Pen a 1 is a major shrimp allergen that induces an allergy. The aim of this study is to assess the effect of simulated gastrointestinal fluids on the allergenicity of Pen a 1. Purified Pen a 1 from shrimp （Penaeus va...Pen a 1 is a major shrimp allergen that induces an allergy. The aim of this study is to assess the effect of simulated gastrointestinal fluids on the allergenicity of Pen a 1. Purified Pen a 1 from shrimp （Penaeus vannamei） was subjected to digestion in SGF （simulated gastric fluid） and SIF （simulated intestinal fluid） for a set time. The allergenicity of digestive Pen a 1 was analyzed by immunoblotting and Ci-ELISA, using pool sera from patients with shrimp specific IgE. The results showed that Pen a 1 exhibited a decrease in allergenicity with increasing digestion time in the SGF and SIF. However, Pen a 1 exhibited strong resistance to digestive fluids, and all yielded fragments （33 kD, 23 kD, and 14 kD） showed allergic activity. Therefore, anti-digestion may be an important factor for Pen a 1 to induce an allergy.展开更多
基金Supported by the National High Technology Research and Development Program of China (863 Program) (NO.2006AA09Z427)the National Natural Science Foundation of China (Nos.30800859,30871948)
文摘Pen a 1 is a major shrimp allergen that induces an allergy. The aim of this study is to assess the effect of simulated gastrointestinal fluids on the allergenicity of Pen a 1. Purified Pen a 1 from shrimp （Penaeus vannamei） was subjected to digestion in SGF （simulated gastric fluid） and SIF （simulated intestinal fluid） for a set time. The allergenicity of digestive Pen a 1 was analyzed by immunoblotting and Ci-ELISA, using pool sera from patients with shrimp specific IgE. The results showed that Pen a 1 exhibited a decrease in allergenicity with increasing digestion time in the SGF and SIF. However, Pen a 1 exhibited strong resistance to digestive fluids, and all yielded fragments （33 kD, 23 kD, and 14 kD） showed allergic activity. Therefore, anti-digestion may be an important factor for Pen a 1 to induce an allergy.
