Acetone–butanol–ethanol(ABE)fermentation process can be exploited for the generation of butanol as biofuel,however it does need to overcome its low volumetric solvent productivity before it can commercially compete ...Acetone–butanol–ethanol(ABE)fermentation process can be exploited for the generation of butanol as biofuel,however it does need to overcome its low volumetric solvent productivity before it can commercially compete with fossil fuel technologies.In this regard,mathematical modelling and simulation analysis are tools that can serve as the base for process engineering development of biological systems.In this work,a novel phenomenological kinetic model of Clostridium acetobutylicum ATCC 824 was considered as a benchmark system to evaluate the behaviour of an ABE fermentation under different process configurations using both free and immobilized cells:single stage batch operation,fed-batch,single stage Continuous Stirred Tank Reactor(CSTR)and multistage CSTRs with and without biomass recirculation.The proposed model achieved a linear correlation index r^2=0.9952 and r^2=0.9710 over experimental data for free and immobilized cells respectively.The predicted maximum butanol concentration and productivity obtained were 13.08 g·L^(-1)and 1.9620 g·L^(-1)·h^(-1)respectively,which represents an increase of 1.01%and 990%versus the currently developed industrial scale process reported currently into the literature.These results provide a reliable platform for the design and optimization of the ABE fermentation system and showcase the adequate predictive nature of the proposed model.展开更多
Batch and continuous fermentation ofgluconic acid production has been studied. The kinetic parameters of the fermentation process were determined from the batch experimental data. The continuous fermentation was model...Batch and continuous fermentation ofgluconic acid production has been studied. The kinetic parameters of the fermentation process were determined from the batch experimental data. The continuous fermentation was modeled to be carried out in a stirred tank reactor. The effect of hydraulic retention time on the steady state continuous fermentation process of glucose by Aspergillus niger to produce gluconic acid was investigated. The result showed that increasing the hydraulic retention time caused the cell amount and gluconic acid concentration at the outlet stream increased but the glucose concentration at the outlet stream decreased. The steady state simulation result was useful for fermenter size determination. Dynamic behaviour of gluconic acid production through fermentation by Aspergillus niger was also studied for a fermenter with 24 h hydraulic retention time. Applying step change of inlet substrate concentration resulted in first order response of cell, substrate and product concentration with all having positive gain. On the other hand, applying step change of inlet cell concentration has resulted in positive gain for cell and product concentration and negative gain for substrate concentration with first order response for all those three parameters.展开更多
基金financial support via the postgraduate scholarship No.277760
文摘Acetone–butanol–ethanol(ABE)fermentation process can be exploited for the generation of butanol as biofuel,however it does need to overcome its low volumetric solvent productivity before it can commercially compete with fossil fuel technologies.In this regard,mathematical modelling and simulation analysis are tools that can serve as the base for process engineering development of biological systems.In this work,a novel phenomenological kinetic model of Clostridium acetobutylicum ATCC 824 was considered as a benchmark system to evaluate the behaviour of an ABE fermentation under different process configurations using both free and immobilized cells:single stage batch operation,fed-batch,single stage Continuous Stirred Tank Reactor(CSTR)and multistage CSTRs with and without biomass recirculation.The proposed model achieved a linear correlation index r^2=0.9952 and r^2=0.9710 over experimental data for free and immobilized cells respectively.The predicted maximum butanol concentration and productivity obtained were 13.08 g·L^(-1)and 1.9620 g·L^(-1)·h^(-1)respectively,which represents an increase of 1.01%and 990%versus the currently developed industrial scale process reported currently into the literature.These results provide a reliable platform for the design and optimization of the ABE fermentation system and showcase the adequate predictive nature of the proposed model.
文摘Batch and continuous fermentation ofgluconic acid production has been studied. The kinetic parameters of the fermentation process were determined from the batch experimental data. The continuous fermentation was modeled to be carried out in a stirred tank reactor. The effect of hydraulic retention time on the steady state continuous fermentation process of glucose by Aspergillus niger to produce gluconic acid was investigated. The result showed that increasing the hydraulic retention time caused the cell amount and gluconic acid concentration at the outlet stream increased but the glucose concentration at the outlet stream decreased. The steady state simulation result was useful for fermenter size determination. Dynamic behaviour of gluconic acid production through fermentation by Aspergillus niger was also studied for a fermenter with 24 h hydraulic retention time. Applying step change of inlet substrate concentration resulted in first order response of cell, substrate and product concentration with all having positive gain. On the other hand, applying step change of inlet cell concentration has resulted in positive gain for cell and product concentration and negative gain for substrate concentration with first order response for all those three parameters.
