目的:探究双盐洗鼻剂鼻腔冲洗用于慢性鼻窦炎(CRS)鼻窦内窥镜术后临床疗效及对血清炎症因子、疼痛视觉模拟量表(VAS)评分的影响。方法:选择2020年3月至2023年5月于唐山中心医院接受鼻窦内镜术的112例CRS患者为研究对象。以随机双盲法分...目的:探究双盐洗鼻剂鼻腔冲洗用于慢性鼻窦炎(CRS)鼻窦内窥镜术后临床疗效及对血清炎症因子、疼痛视觉模拟量表(VAS)评分的影响。方法:选择2020年3月至2023年5月于唐山中心医院接受鼻窦内镜术的112例CRS患者为研究对象。以随机双盲法分为对照组和观察组,每组56例,对照组患者于术后接受300 mL 3%高渗盐水等常规治疗,观察组患者术后联合双盐洗鼻剂鼻腔冲洗治疗。比较两组患者疗效,并分析两组患者治疗前后血清炎性因子、围手术期生活质量(SNOT-20)和VAS评分。结果:治疗后,与对照组总有效率82.14%比较,观察组总有效率94.64%更高,差异有统计学意义(P<0.05);治疗后,观察组治疗后1、2个月的VAS评分[(5.84±1.89)分、(3.98±1.26)分]、SNOT-20评分[(2.77±0.41)分]以及IL-5[(3.28±0.94)ng·mL^(-1)]和IL-6[(85.12±11.24)ng·L^(-1)],低于对照组的[(6.71±1.64)分、(5.12±1.17)分、(4.45±0.72)分、(5.78±1.23)ng·mL^(-1)、(60.84±5.20)ng·L^(-1)],差异有统计学意义(P<0.05);治疗期间,两组患者不良反应事件发生率分别为12.50%、5.36%,差异无统计学意义(P>0.05)。结论:双盐洗鼻剂鼻腔冲洗用于CRS患者鼻窦内镜术后治疗,可显著提升CRS患者疗效,改善疼痛症状和生活质量,稳定患者机体内血清炎性因子水平。展开更多
Baicalein, baicalin, scutellarin and Chrysin-7-O-β-D-glucuronide are the major flavonoids of the Shuang-huang-lian powder injection. These flavonoids are thought to be haptens that can induce allergic reactions. The ...Baicalein, baicalin, scutellarin and Chrysin-7-O-β-D-glucuronide are the major flavonoids of the Shuang-huang-lian powder injection. These flavonoids are thought to be haptens that can induce allergic reactions. The interaction mechanism of these haptens with bovine serum albumin （BSA） was investigated by surface plasmon resonance （SPR） and molecular modeling method. The SPR study indicated that these compounds could specifically bind to the BSA with one binding site and equilibrium dissociation constant （KD） values were determined. Molecular modeling explored the mechanism of interaction under simulated physiological condition. The result of molecular modeling indicated that flavonoids could bind with BSA in the hydrophobic pocket of sub-domain II with hydrogen bonding as the main acting force.展开更多
Objective:To study the effect of Chinese herbal medicine Kangxin Fumai Granule(康心复脉颗粒 Granule for heart diseases) serum on the primary cultured sinoatrial node(SAN) cell apoptosis induced by simulated ischemia-r...Objective:To study the effect of Chinese herbal medicine Kangxin Fumai Granule(康心复脉颗粒 Granule for heart diseases) serum on the primary cultured sinoatrial node(SAN) cell apoptosis induced by simulated ischemia-reperfusion(IR).Methods:The SAN cells removed from SAN tissue of neonatal Wistar rats were cultured and purified with differential attachment and 5'-bromodeoxyuridine(BrdU) treatment.Simulated IR model was adopted.The obtained cells were morphologically observed with inverted microscopy.By using the method of serum pharmacology,the cell apoptosis was measured with TUNEL staining qualitatively and with flow cytometry quantitatively.Results:Three kinds of cells were observed in the cultured SAN cells:spindle,triangle and irregular.The spindle cells comprised the greatest proportion.The SAN cells in the model group showed moderate positive brown staining in the nucleus,and the apoptosis rate increased significantly compared to that in the control group(P<0.01).While the SAN cells in the Kangxin Fumai Granule high-dose group did not demonstrated positive staining in the nucleus,and the apoptosis rate decreased significantly compared to that in the model group(P<0.05).Conclusion:Of the cells cultured from SAN,the spindle cells were pacemaker cells of SAN in rats.Blockade and/or inhibition of the SAN cell apoptosis might be one of the important mechanisms of Kangxin Fumai Granule in preventing and treating sinoatrial injury induced by simulated IR.展开更多
文摘目的:探究双盐洗鼻剂鼻腔冲洗用于慢性鼻窦炎(CRS)鼻窦内窥镜术后临床疗效及对血清炎症因子、疼痛视觉模拟量表(VAS)评分的影响。方法:选择2020年3月至2023年5月于唐山中心医院接受鼻窦内镜术的112例CRS患者为研究对象。以随机双盲法分为对照组和观察组,每组56例,对照组患者于术后接受300 mL 3%高渗盐水等常规治疗,观察组患者术后联合双盐洗鼻剂鼻腔冲洗治疗。比较两组患者疗效,并分析两组患者治疗前后血清炎性因子、围手术期生活质量(SNOT-20)和VAS评分。结果:治疗后,与对照组总有效率82.14%比较,观察组总有效率94.64%更高,差异有统计学意义(P<0.05);治疗后,观察组治疗后1、2个月的VAS评分[(5.84±1.89)分、(3.98±1.26)分]、SNOT-20评分[(2.77±0.41)分]以及IL-5[(3.28±0.94)ng·mL^(-1)]和IL-6[(85.12±11.24)ng·L^(-1)],低于对照组的[(6.71±1.64)分、(5.12±1.17)分、(4.45±0.72)分、(5.78±1.23)ng·mL^(-1)、(60.84±5.20)ng·L^(-1)],差异有统计学意义(P<0.05);治疗期间,两组患者不良反应事件发生率分别为12.50%、5.36%,差异无统计学意义(P>0.05)。结论:双盐洗鼻剂鼻腔冲洗用于CRS患者鼻窦内镜术后治疗,可显著提升CRS患者疗效,改善疼痛症状和生活质量,稳定患者机体内血清炎性因子水平。
基金Nature Science Foundation of Beijing,China(Grant No.7142088)
文摘Baicalein, baicalin, scutellarin and Chrysin-7-O-β-D-glucuronide are the major flavonoids of the Shuang-huang-lian powder injection. These flavonoids are thought to be haptens that can induce allergic reactions. The interaction mechanism of these haptens with bovine serum albumin （BSA） was investigated by surface plasmon resonance （SPR） and molecular modeling method. The SPR study indicated that these compounds could specifically bind to the BSA with one binding site and equilibrium dissociation constant （KD） values were determined. Molecular modeling explored the mechanism of interaction under simulated physiological condition. The result of molecular modeling indicated that flavonoids could bind with BSA in the hydrophobic pocket of sub-domain II with hydrogen bonding as the main acting force.
基金supported by the fund from the State Administration of Traditional Chinese Medicine (No.04-05JP61)
文摘Objective:To study the effect of Chinese herbal medicine Kangxin Fumai Granule(康心复脉颗粒 Granule for heart diseases) serum on the primary cultured sinoatrial node(SAN) cell apoptosis induced by simulated ischemia-reperfusion(IR).Methods:The SAN cells removed from SAN tissue of neonatal Wistar rats were cultured and purified with differential attachment and 5'-bromodeoxyuridine(BrdU) treatment.Simulated IR model was adopted.The obtained cells were morphologically observed with inverted microscopy.By using the method of serum pharmacology,the cell apoptosis was measured with TUNEL staining qualitatively and with flow cytometry quantitatively.Results:Three kinds of cells were observed in the cultured SAN cells:spindle,triangle and irregular.The spindle cells comprised the greatest proportion.The SAN cells in the model group showed moderate positive brown staining in the nucleus,and the apoptosis rate increased significantly compared to that in the control group(P<0.01).While the SAN cells in the Kangxin Fumai Granule high-dose group did not demonstrated positive staining in the nucleus,and the apoptosis rate decreased significantly compared to that in the model group(P<0.05).Conclusion:Of the cells cultured from SAN,the spindle cells were pacemaker cells of SAN in rats.Blockade and/or inhibition of the SAN cell apoptosis might be one of the important mechanisms of Kangxin Fumai Granule in preventing and treating sinoatrial injury induced by simulated IR.
