在研究获取产品模板数据方式、多用户同时使用、解析产品模板数据生成GUI等技术基础上,以NX为开发平台,NX/Open、Eclipse、Google Web Toolkit为开发工具,Jetty作为服务端容器,开发了基于服务端、客户端的产品模板配置器,通过该系统可...在研究获取产品模板数据方式、多用户同时使用、解析产品模板数据生成GUI等技术基础上,以NX为开发平台,NX/Open、Eclipse、Google Web Toolkit为开发工具,Jetty作为服务端容器,开发了基于服务端、客户端的产品模板配置器,通过该系统可以读取并解析产品模板数据,在客户端上生成相应的用户界面和显示三维模型,从而在产品数据共享的同时,保证了数据的安全,同时降低了企业的硬件成本和维护成本。展开更多
In order to reduce product development cycle time, aerospace companies tend to develop various correlations integrating geometric and performance parameters. This paper covers the development of a parameterization mod...In order to reduce product development cycle time, aerospace companies tend to develop various correlations integrating geometric and performance parameters. This paper covers the development of a parameterization modeling, to be used in the preliminary design phase, for the turbine cover plate of an aero-engine. The parameterization modeling of the turbine cover plate is achieved by using commercial CAD （computer aided design） software processing in batch mode. Two main approaches are presented the outer face and the skeleton models. These models can then be integrated into an iterative process for designing optimal shapes. Both models are capable of reproducing existing cover plate with reasonable accuracy in relatively shorter time periods. However, the skeleton approach provides probably the best results in terms of flexibility and accuracy, but increases programming complexity and requires greater run times.展开更多
DNA methylation, catalyzed by DNA methyltransferases(MTases), is a key component of genetic regulation, and DNA MTases have been regarded as potential targets in anticancer therapy. Herein, based on our previously dev...DNA methylation, catalyzed by DNA methyltransferases(MTases), is a key component of genetic regulation, and DNA MTases have been regarded as potential targets in anticancer therapy. Herein, based on our previously developed DNA-mediated supercharged green fluorescent protein(Sc GFP)/graphene oxide(GO) interaction, coupled with methylation-initiated template-free DNA polymerization, we propose a novel fluorescence assay strategy for sensitive detection of DNA MTase activity. A hairpin DNA with a methylation-sensitive site and an amino-modified 3′-terminal(DNA-1) was designed and worked as a starting molecule. In the presence of DNA MTase, methylation-sensitive restriction endonuclease, and terminal deoxynucleotidyl transferase(Td T), DNA-1 can be sequentially methylated, cleaved, and further elongated. The resulting long DNA fragments quickly bind with Sc GFP and form the Sc GFP/DNA nanocomplex. Such nanocomplex can effectively protect Sc GFP from being adsorbed and quenched by GO. Without the methylation-initiated DNA polymerization, the fluorescence of Sc GFP will be quenched by GO. Thus, the DNA MTase activity, which is proportional to the amount of DNA polymerization products, can be measured by reading the fluorescence of Sc GFP/GO. The method was successfully used to detect the activity of DNA adenine methylation(Dam) MTase with a wide linear range(0.1–100 U/m L) and a low detection limit of 0.1 U/m L. In addition, the method showed high selectivity and the potential to be applied in a complex sample. Furthermore, this study was successfully extended to evaluate the inhibition effect of 5-fluorouracil on Dam MTase activity and detect Td T activity.展开更多
文摘在研究获取产品模板数据方式、多用户同时使用、解析产品模板数据生成GUI等技术基础上,以NX为开发平台,NX/Open、Eclipse、Google Web Toolkit为开发工具,Jetty作为服务端容器,开发了基于服务端、客户端的产品模板配置器,通过该系统可以读取并解析产品模板数据,在客户端上生成相应的用户界面和显示三维模型,从而在产品数据共享的同时,保证了数据的安全,同时降低了企业的硬件成本和维护成本。
文摘In order to reduce product development cycle time, aerospace companies tend to develop various correlations integrating geometric and performance parameters. This paper covers the development of a parameterization modeling, to be used in the preliminary design phase, for the turbine cover plate of an aero-engine. The parameterization modeling of the turbine cover plate is achieved by using commercial CAD （computer aided design） software processing in batch mode. Two main approaches are presented the outer face and the skeleton models. These models can then be integrated into an iterative process for designing optimal shapes. Both models are capable of reproducing existing cover plate with reasonable accuracy in relatively shorter time periods. However, the skeleton approach provides probably the best results in terms of flexibility and accuracy, but increases programming complexity and requires greater run times.
基金supported by the National Basic Research Program (2011CB911002)the National Natural Science Foundation of China (21190044, 21475037, 21222507, 21175036)the fundamental research funds for the central universities
文摘DNA methylation, catalyzed by DNA methyltransferases(MTases), is a key component of genetic regulation, and DNA MTases have been regarded as potential targets in anticancer therapy. Herein, based on our previously developed DNA-mediated supercharged green fluorescent protein(Sc GFP)/graphene oxide(GO) interaction, coupled with methylation-initiated template-free DNA polymerization, we propose a novel fluorescence assay strategy for sensitive detection of DNA MTase activity. A hairpin DNA with a methylation-sensitive site and an amino-modified 3′-terminal(DNA-1) was designed and worked as a starting molecule. In the presence of DNA MTase, methylation-sensitive restriction endonuclease, and terminal deoxynucleotidyl transferase(Td T), DNA-1 can be sequentially methylated, cleaved, and further elongated. The resulting long DNA fragments quickly bind with Sc GFP and form the Sc GFP/DNA nanocomplex. Such nanocomplex can effectively protect Sc GFP from being adsorbed and quenched by GO. Without the methylation-initiated DNA polymerization, the fluorescence of Sc GFP will be quenched by GO. Thus, the DNA MTase activity, which is proportional to the amount of DNA polymerization products, can be measured by reading the fluorescence of Sc GFP/GO. The method was successfully used to detect the activity of DNA adenine methylation(Dam) MTase with a wide linear range(0.1–100 U/m L) and a low detection limit of 0.1 U/m L. In addition, the method showed high selectivity and the potential to be applied in a complex sample. Furthermore, this study was successfully extended to evaluate the inhibition effect of 5-fluorouracil on Dam MTase activity and detect Td T activity.
