Objective: To develop a new method for the detection of TPMT gene mutations and determine the frequencies of four TPMT alleles, TPMT *1, *3A , *3B and *3C in a healthy Chinese population. Methods: A TDI-FP assay syste...Objective: To develop a new method for the detection of TPMT gene mutations and determine the frequencies of four TPMT alleles, TPMT *1, *3A , *3B and *3C in a healthy Chinese population. Methods: A TDI-FP assay system was set up in out lab. To evaluate this system, 220 healthy individuals were analyzed for the polymorphic sites at positions 460(G→A)and 719(A→G)of the TPMP gene using our new TDI–FP method. Results: Three TPMP*3C(G 460→G 719) heterozygotes were identified, TPMP *3A and TPMP *3B were not found. All mutations were confirmed by conventional DNA sequencing analysis. Conclusion: TDI-FP method has proven to be very efficient as a rapid and accurate approach for TPMP genotyping. TPMP *3C was the only polymorphism identified in this clinical samples we have registered.展开更多
A quantitative structure-spectrum relationship (QSSR) model was developed to simulate 13C nuclear magnetic resonance (NMR) spectra of carbinol carbon atoms for 55 alcohols. The proposed model,using multiple linear reg...A quantitative structure-spectrum relationship (QSSR) model was developed to simulate 13C nuclear magnetic resonance (NMR) spectra of carbinol carbon atoms for 55 alcohols. The proposed model,using multiple linear regression,contained four descriptors solely extracted from the molecular structure of compounds. The statistical results of the final model show that R2= 0.982 4 and S=0.869 8 (where R is the correlation coefficient and S is the standard deviation). To test its predictive ability,the model was further used to predict the 13C NMR spectra of the carbinol carbon atoms of other nine compounds which were not included in the developed model. The average relative errors are 0.94% and 1.70%,respectively,for the training set and the predictive set. The model is statistically significant and shows good stability for data variation as tested by the leave-one-out (LOO) cross-validation. The comparison with other approaches also reveals good performance of this method.展开更多
In the field of converting simulation surveying and traditional close range photogrammetry, it has been developed so far to survey objects by commercial digital camera and this technique is applied widely in every par...In the field of converting simulation surveying and traditional close range photogrammetry, it has been developed so far to survey objects by commercial digital camera and this technique is applied widely in every part of production. In order to get three-dimensional information of objects, commercial digital camera must be examined. For a long time, digital camera has been examined by DLT. Then there must be a high-precision control field. For realizing surveying without control points, a method for self-calibration is proposed.展开更多
In the discipline of geotechnical engineering, fiber optic sensor based distributed monitoring has played an increasingly important role over the past few decades. Compared with conventional sensors, fiber optic senso...In the discipline of geotechnical engineering, fiber optic sensor based distributed monitoring has played an increasingly important role over the past few decades. Compared with conventional sensors, fiber optic sensors have a variety of exclusive advantages, such as smaller size, higher precision, and better corrosion resistance. These innovative monitoring technologies have been successfully applied for performance monitoring of geo-structures and early warning of potential geo- hazards around the world. In order to investigate their ability to monitor slope stability problems, a medium-sized model of soil nailed slope has been constructed in laboratory. The fully distributed Brillouin optical time-domain analysis （BOTDA） sensing technology was employed to measure the horizontal strain distributions inside the model slope. During model construction, a specially designed strain sensing fiber was buried in the soil mass. Afterward, the surcharge loading was applied on the slope crest in stages using hydraulic jacks and a reaction frame. During testing, an NBX-6o5o BOTDA sensing interrogator was used to collect the fiber optic sensing data. The test results have been analyzed in detail, which shows that the fiber optic sensors can capture the progressive deformation and failure pattern of the model slope. The limit equilibrium analyses were also conducted to obtain the factors ofsafety of the slope under different surface loadings. It is found that the characteristic maximum strains can reflect the stability of the model slope and an empirical relationship was obtained, This study verified the effectiveness of the distributed BOTDA sensing technology in performance monitoring of slope.展开更多
The contents ofMg, Al, Si, Ti, Cr, Mn, Fe, Co, Cu, Ga, As, Se, Cd, Sb, Pb and Bi in high purity nickel were determined by high resolution inductively coupled plasma mass spectrometry （HR-ICP-MS）. The sample was diss...The contents ofMg, Al, Si, Ti, Cr, Mn, Fe, Co, Cu, Ga, As, Se, Cd, Sb, Pb and Bi in high purity nickel were determined by high resolution inductively coupled plasma mass spectrometry （HR-ICP-MS）. The sample was dissolved in HNO3 and HCI by microwave digestion. Most of the spectral interferences could be avoided by measuring in the high resolution mode. The matrix effects because of the presence of excess HC1 and nickel were evaluated. Correction for matrix effects was made using Sc, Rh and T1 as internal standards. The optimum conditions for the determination were tested and discussed. The detection limits range from 0.012 to 1.76 ~tg/g depending on the type of elements. The applicability of the proposed method is also validated by the analysis of high purity nickel reference material （NIST SRM 671）. The relative standard deviation （RSD） is less than 3.3%. Results for determination of trace elements in high purity nickel were presented.展开更多
The changes in a selection of extreme climate indices(maximum of daily maximum temperature(TXx),minimum of daily minimum temperature(TNn),annual total precipitation when the daily precipitation exceeds the 95th percen...The changes in a selection of extreme climate indices(maximum of daily maximum temperature(TXx),minimum of daily minimum temperature(TNn),annual total precipitation when the daily precipitation exceeds the 95th percentile of wet-day precipitation(very wet days,R95p),and the maximum number of consecutive days with less than 1 mm of precipitation(consecutive dry days,CDD))were projected using multi-model results from phase 5 of the Coupled Model Intercomparison Project in the early,middle,and latter parts of the 21st century under different Representative Concentration Pathway(RCP)emissions scenarios.The results suggest that TXx and TNn will increase in the future and,moreover,the increases of TNn under all RCPs are larger than those of TXx.R95p is projected to increase and CDD to decrease significantly.The changes in TXx,TNn,R95p,and CDD in eight sub-regions of China are different in the three periods of the 21st century,and the ranges of change for the four indices under the higher emissions scenario are projected to be larger than those under the lower emissions scenario.The multi-model simulations show remarkable consistency in their projection of the extreme temperature indices,but poor consistency with respect to the extreme precipitation indices.More substantial inconsistency is found in those regions where high and low temperatures are likely to happen for TXx and TNn,respectively.For extreme precipitation events(R95p),greater uncertainty appears in most of the southern regions,while for drought events(CDD)it appears in the basins of Xinjiang.The uncertainty in the future changes of the extreme climate indices increases with the increasing severity of the emissions scenario.展开更多
文摘Objective: To develop a new method for the detection of TPMT gene mutations and determine the frequencies of four TPMT alleles, TPMT *1, *3A , *3B and *3C in a healthy Chinese population. Methods: A TDI-FP assay system was set up in out lab. To evaluate this system, 220 healthy individuals were analyzed for the polymorphic sites at positions 460(G→A)and 719(A→G)of the TPMP gene using our new TDI–FP method. Results: Three TPMP*3C(G 460→G 719) heterozygotes were identified, TPMP *3A and TPMP *3B were not found. All mutations were confirmed by conventional DNA sequencing analysis. Conclusion: TDI-FP method has proven to be very efficient as a rapid and accurate approach for TPMP genotyping. TPMP *3C was the only polymorphism identified in this clinical samples we have registered.
基金Projects(20775010, 21075011) supported by the National Natural Science Foundation of ChinaProject(2008AA05Z405) supported by the National High-tech Research and Development Program of China+2 种基金Project(09JJ3016) supported by the Natural Science Foundation of Hunan Province, ChinaProject(09C066) supported by the Scientific Research Fund of Hunan Provincial Education Department, ChinaProject(2010CL01) supported by the Foundation of Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, China
文摘A quantitative structure-spectrum relationship (QSSR) model was developed to simulate 13C nuclear magnetic resonance (NMR) spectra of carbinol carbon atoms for 55 alcohols. The proposed model,using multiple linear regression,contained four descriptors solely extracted from the molecular structure of compounds. The statistical results of the final model show that R2= 0.982 4 and S=0.869 8 (where R is the correlation coefficient and S is the standard deviation). To test its predictive ability,the model was further used to predict the 13C NMR spectra of the carbinol carbon atoms of other nine compounds which were not included in the developed model. The average relative errors are 0.94% and 1.70%,respectively,for the training set and the predictive set. The model is statistically significant and shows good stability for data variation as tested by the leave-one-out (LOO) cross-validation. The comparison with other approaches also reveals good performance of this method.
文摘In the field of converting simulation surveying and traditional close range photogrammetry, it has been developed so far to survey objects by commercial digital camera and this technique is applied widely in every part of production. In order to get three-dimensional information of objects, commercial digital camera must be examined. For a long time, digital camera has been examined by DLT. Then there must be a high-precision control field. For realizing surveying without control points, a method for self-calibration is proposed.
基金the financial support provided by the National Basic Research Program of China (973 Program) (Grant No. 2011CB710605)the National Natural Science Foundation of China (Grant Nos. 41102174, 41302217)supported by the National Key Technology R&D Program of China (Grant No. 2012BAK10B05)
文摘In the discipline of geotechnical engineering, fiber optic sensor based distributed monitoring has played an increasingly important role over the past few decades. Compared with conventional sensors, fiber optic sensors have a variety of exclusive advantages, such as smaller size, higher precision, and better corrosion resistance. These innovative monitoring technologies have been successfully applied for performance monitoring of geo-structures and early warning of potential geo- hazards around the world. In order to investigate their ability to monitor slope stability problems, a medium-sized model of soil nailed slope has been constructed in laboratory. The fully distributed Brillouin optical time-domain analysis （BOTDA） sensing technology was employed to measure the horizontal strain distributions inside the model slope. During model construction, a specially designed strain sensing fiber was buried in the soil mass. Afterward, the surcharge loading was applied on the slope crest in stages using hydraulic jacks and a reaction frame. During testing, an NBX-6o5o BOTDA sensing interrogator was used to collect the fiber optic sensing data. The test results have been analyzed in detail, which shows that the fiber optic sensors can capture the progressive deformation and failure pattern of the model slope. The limit equilibrium analyses were also conducted to obtain the factors ofsafety of the slope under different surface loadings. It is found that the characteristic maximum strains can reflect the stability of the model slope and an empirical relationship was obtained, This study verified the effectiveness of the distributed BOTDA sensing technology in performance monitoring of slope.
基金Project(21075138) supported by the National Natural Science Foundation of ChinaProject(cstc2011jjA0780) supported by Natural Science Foundation of Chongqing City,ChinaProject(KJ121311) supported by Educational Commission of Chongqing City of China
文摘The contents ofMg, Al, Si, Ti, Cr, Mn, Fe, Co, Cu, Ga, As, Se, Cd, Sb, Pb and Bi in high purity nickel were determined by high resolution inductively coupled plasma mass spectrometry （HR-ICP-MS）. The sample was dissolved in HNO3 and HCI by microwave digestion. Most of the spectral interferences could be avoided by measuring in the high resolution mode. The matrix effects because of the presence of excess HC1 and nickel were evaluated. Correction for matrix effects was made using Sc, Rh and T1 as internal standards. The optimum conditions for the determination were tested and discussed. The detection limits range from 0.012 to 1.76 ~tg/g depending on the type of elements. The applicability of the proposed method is also validated by the analysis of high purity nickel reference material （NIST SRM 671）. The relative standard deviation （RSD） is less than 3.3%. Results for determination of trace elements in high purity nickel were presented.
基金supported by the R&D Special Fund for Public Welfare Industry (Meteorology) (Grant No. GYHY201306019)the National Natural Science Foundation of China (Grant No. 41275078)
文摘The changes in a selection of extreme climate indices(maximum of daily maximum temperature(TXx),minimum of daily minimum temperature(TNn),annual total precipitation when the daily precipitation exceeds the 95th percentile of wet-day precipitation(very wet days,R95p),and the maximum number of consecutive days with less than 1 mm of precipitation(consecutive dry days,CDD))were projected using multi-model results from phase 5 of the Coupled Model Intercomparison Project in the early,middle,and latter parts of the 21st century under different Representative Concentration Pathway(RCP)emissions scenarios.The results suggest that TXx and TNn will increase in the future and,moreover,the increases of TNn under all RCPs are larger than those of TXx.R95p is projected to increase and CDD to decrease significantly.The changes in TXx,TNn,R95p,and CDD in eight sub-regions of China are different in the three periods of the 21st century,and the ranges of change for the four indices under the higher emissions scenario are projected to be larger than those under the lower emissions scenario.The multi-model simulations show remarkable consistency in their projection of the extreme temperature indices,but poor consistency with respect to the extreme precipitation indices.More substantial inconsistency is found in those regions where high and low temperatures are likely to happen for TXx and TNn,respectively.For extreme precipitation events(R95p),greater uncertainty appears in most of the southern regions,while for drought events(CDD)it appears in the basins of Xinjiang.The uncertainty in the future changes of the extreme climate indices increases with the increasing severity of the emissions scenario.
