探索一种适于橄榄果实游离氨基酸含量测定的方法。通过单因素实验初筛茚三酮显色法中的条件,如p H、显色剂含量、加热温度和加热时间,并通过稳定性、精密性、重复性和加样回收实验进行方法学验证。结果发现:茚三酮显色法的最佳反应条件...探索一种适于橄榄果实游离氨基酸含量测定的方法。通过单因素实验初筛茚三酮显色法中的条件,如p H、显色剂含量、加热温度和加热时间,并通过稳定性、精密性、重复性和加样回收实验进行方法学验证。结果发现:茚三酮显色法的最佳反应条件为最大吸收波长568 nm、p H6.6的磷酸缓冲液、0.5 m L 2%茚三酮溶液、加热温度100℃和加热时间25 min,在30 min内OD值改变0.41%,稳定性RSD=0.34%(n=9)、精密性RSD=0.09%(n=9)、重复性RSD=1.71%(n=6)、回收率达100.34%、RSD=2.21%(n=6)。表明茚三酮显色法测定橄榄果实游离氨基酸含量在此条件下稳定且精密性、重复性和回收率良好,可用于橄榄果实游离氨基酸的快速简便测定。展开更多
为筛选出能够改善橄榄采后鲜食品质的合适的冷激处理时间及紫外辐照(UV-C)剂量,以长营橄榄为材料,对果实进行10~50 min的冷激处理,0.6~4.8 k J/m2的紫外辐照。并对其鲜食品质相关指标(总酚、总糖、黄酮)含量进行测定。结果表明:冷激处...为筛选出能够改善橄榄采后鲜食品质的合适的冷激处理时间及紫外辐照(UV-C)剂量,以长营橄榄为材料,对果实进行10~50 min的冷激处理,0.6~4.8 k J/m2的紫外辐照。并对其鲜食品质相关指标(总酚、总糖、黄酮)含量进行测定。结果表明:冷激处理及紫外辐照在一定程度上降低了橄榄果实中总酚、黄酮含量;冷激处理及0.6~2.4 k J/m2UV-C辐照延缓了果实中总糖含量的下降,而3.6~4.8 k J/m2UV-C辐照则加速了果实中总糖的消耗。其中,30 min的冷激处理及2.4 k J/m2UV-C辐照可以维持果实中较低的酚糖比,对于改善果实口感、提高其鲜食品质具有积极的作用。展开更多
[Objective] This study aimed to identify the difference in squalene content among different olive varieties and the law of squalene content change in the same olive variety of different degrees of maturity, with the o...[Objective] This study aimed to identify the difference in squalene content among different olive varieties and the law of squalene content change in the same olive variety of different degrees of maturity, with the objective to provide technical support for the harvest and processing of olive fruits. [Method] Taking 13 introduced olive varieties including three varieties of eight different grades of maturity, fat acid w3s first extracted by Soxhlet extraction and then squalene was quantitatively and qualitatively analyzed by gas chromatography-mass spectrometry （GC-MS） method. [Result] The results showed that squalene content was the highest in CG-32, and the lowest in Leccino among the 13 olive varieties. And squalene content increased with the degree of maturity in the three olive varieties including CG-32, Frantoio and Ascolana Tenera. [Conclusion] A more simple and rapid method for the determina- tion of squatene content was established.展开更多
文摘探索一种适于橄榄果实游离氨基酸含量测定的方法。通过单因素实验初筛茚三酮显色法中的条件,如p H、显色剂含量、加热温度和加热时间,并通过稳定性、精密性、重复性和加样回收实验进行方法学验证。结果发现:茚三酮显色法的最佳反应条件为最大吸收波长568 nm、p H6.6的磷酸缓冲液、0.5 m L 2%茚三酮溶液、加热温度100℃和加热时间25 min,在30 min内OD值改变0.41%,稳定性RSD=0.34%(n=9)、精密性RSD=0.09%(n=9)、重复性RSD=1.71%(n=6)、回收率达100.34%、RSD=2.21%(n=6)。表明茚三酮显色法测定橄榄果实游离氨基酸含量在此条件下稳定且精密性、重复性和回收率良好,可用于橄榄果实游离氨基酸的快速简便测定。
文摘为筛选出能够改善橄榄采后鲜食品质的合适的冷激处理时间及紫外辐照(UV-C)剂量,以长营橄榄为材料,对果实进行10~50 min的冷激处理,0.6~4.8 k J/m2的紫外辐照。并对其鲜食品质相关指标(总酚、总糖、黄酮)含量进行测定。结果表明:冷激处理及紫外辐照在一定程度上降低了橄榄果实中总酚、黄酮含量;冷激处理及0.6~2.4 k J/m2UV-C辐照延缓了果实中总糖含量的下降,而3.6~4.8 k J/m2UV-C辐照则加速了果实中总糖的消耗。其中,30 min的冷激处理及2.4 k J/m2UV-C辐照可以维持果实中较低的酚糖比,对于改善果实口感、提高其鲜食品质具有积极的作用。
基金Supported by the Key New Products Development Plan Project of Yunnan Province(2009BB006)~~
文摘[Objective] This study aimed to identify the difference in squalene content among different olive varieties and the law of squalene content change in the same olive variety of different degrees of maturity, with the objective to provide technical support for the harvest and processing of olive fruits. [Method] Taking 13 introduced olive varieties including three varieties of eight different grades of maturity, fat acid w3s first extracted by Soxhlet extraction and then squalene was quantitatively and qualitatively analyzed by gas chromatography-mass spectrometry （GC-MS） method. [Result] The results showed that squalene content was the highest in CG-32, and the lowest in Leccino among the 13 olive varieties. And squalene content increased with the degree of maturity in the three olive varieties including CG-32, Frantoio and Ascolana Tenera. [Conclusion] A more simple and rapid method for the determina- tion of squatene content was established.
