There are more than 6 000 clones of Hema brasiliensis Mull. Ary germplasm in the germplasm garden of Chinese National Key Biotechnology Laboratory for Tropical Crops and some of them are elite germplasm demonstrated b...There are more than 6 000 clones of Hema brasiliensis Mull. Ary germplasm in the germplasm garden of Chinese National Key Biotechnology Laboratory for Tropical Crops and some of them are elite germplasm demonstrated by production and previous studies. AFLP (amplified fragment length polymorphism) fingerprinting analysis was performed on 25 clones (15 of Wickham clones and 10 of Amazon wild clones which possess phenotypes with high-yielding,/Iow-yielding, cold-tolerance/cold-sensitivity, oidium-resistance/oidium-sensitivity, tapping panel dryness (TPD) /healthy) respectively through a 377 DNA sequencer (P. E. Corp.) and PAGE electrophoresis results were analyzed by using GeneScan(TM) and Genotype(TM) Analysis software (P. E. Corp. The fragment profiles of different clones were obtained. Five hundred and eighteen fragments were generated by two primer combinations screened from 64 primer combinations and 511 fragments appeared to be polymorphic (98.6%). Genetic distance ranged from 0.25 to 0.81 between clones and ranged from 0.07 to 0.17 within RRIM600 clone. A specific 320 bp fragment of the oidium-resistant clones was found through genotype analysis. These results showed that AFLP fingerprints were highly reproducible and powerful and can be widely used in germplasm identification and genetic diversity analysis of Hema brasiliensis. In addition, based on the AFLP data, cluster analysis was performed. Cluster results showed that all the clones studied were almost clustered into a group one by one.展开更多
A model to describe the hysteresis damper character of rubber material is presented in this paper. It consists of a parallel spring and damper, whose coefficients change with vibration frequencies. In order to acquire...A model to describe the hysteresis damper character of rubber material is presented in this paper. It consists of a parallel spring and damper, whose coefficients change with vibration frequencies. In order to acquire these relations, the force decomposition is carried out according to some sine vibration measurement data about nonlinear forces changing with deformations of the rubber material. The nonlinear force is decomposed into a spring force and a damper force, which are represented by a frcquency-dependent spring and damper coefficient, respectively. Repeating this step for different measurements will give different coefficients corresponding to different frequencies. Then, application of a parameter identification method will provide the requested functions over frequency. Using those formulae, as an example, the dynamic character of a hollow shaft system supported by rubber rings is analyzed and the acceleration response curve in the centroid position is calculated. Comparisons with sine vibration experiments of the real system show a maximal inaccuracy of 8. 8 %. Application of this model and procedure can simplify the modeling and analysis of mechanical systems including rubber materials.展开更多
Seismic behaviors of base-isolated structures are highly affected by the nonlinear characteristics of the isolated systems. Most of the currently available methods for the identification of nonlinear properties of iso...Seismic behaviors of base-isolated structures are highly affected by the nonlinear characteristics of the isolated systems. Most of the currently available methods for the identification of nonlinear properties of isolator require either the measurements of all structural responses or the assumptions of the proper mathematic models for the rubber-bearings. In this paper, two algorithms are proposed to identify the nonlinear properties of rubber-bearings in base-isolated buildings using only partial measurements of structural dynamic responses. The first algorithm is applicable to the case that proper mathematical models are available for the base isolators. It is based on the extended Kalman filter for the parametric identification of nonlinear models of rubber-bearing isolators and buildings. For the general case where it is difficult to establish a proper mathematical model to describe the nonlinear behavior of a rubber-bearing isolator, another algorithm is proposed to identify the model-tYee nonlinear property of rubber-bearing isolated system. Nonlinear effect of rubber-bearing is treated as ＇fictitious loading＇ on the linear building under severe earthquake. The algorithm is based on the sequential Kalman estimator for the structural responses and the least-squares estimation of the ＇fictitious loading＇ to identify the nonlinear force of rubber-bearing isolator. Simulation results demonstrate that the proposed two algorithms are capable of identifying the nonlinear properties of rubber-bearing isolated systems with good accuracy.展开更多
文摘There are more than 6 000 clones of Hema brasiliensis Mull. Ary germplasm in the germplasm garden of Chinese National Key Biotechnology Laboratory for Tropical Crops and some of them are elite germplasm demonstrated by production and previous studies. AFLP (amplified fragment length polymorphism) fingerprinting analysis was performed on 25 clones (15 of Wickham clones and 10 of Amazon wild clones which possess phenotypes with high-yielding,/Iow-yielding, cold-tolerance/cold-sensitivity, oidium-resistance/oidium-sensitivity, tapping panel dryness (TPD) /healthy) respectively through a 377 DNA sequencer (P. E. Corp.) and PAGE electrophoresis results were analyzed by using GeneScan(TM) and Genotype(TM) Analysis software (P. E. Corp. The fragment profiles of different clones were obtained. Five hundred and eighteen fragments were generated by two primer combinations screened from 64 primer combinations and 511 fragments appeared to be polymorphic (98.6%). Genetic distance ranged from 0.25 to 0.81 between clones and ranged from 0.07 to 0.17 within RRIM600 clone. A specific 320 bp fragment of the oidium-resistant clones was found through genotype analysis. These results showed that AFLP fingerprints were highly reproducible and powerful and can be widely used in germplasm identification and genetic diversity analysis of Hema brasiliensis. In addition, based on the AFLP data, cluster analysis was performed. Cluster results showed that all the clones studied were almost clustered into a group one by one.
文摘A model to describe the hysteresis damper character of rubber material is presented in this paper. It consists of a parallel spring and damper, whose coefficients change with vibration frequencies. In order to acquire these relations, the force decomposition is carried out according to some sine vibration measurement data about nonlinear forces changing with deformations of the rubber material. The nonlinear force is decomposed into a spring force and a damper force, which are represented by a frcquency-dependent spring and damper coefficient, respectively. Repeating this step for different measurements will give different coefficients corresponding to different frequencies. Then, application of a parameter identification method will provide the requested functions over frequency. Using those formulae, as an example, the dynamic character of a hollow shaft system supported by rubber rings is analyzed and the acceleration response curve in the centroid position is calculated. Comparisons with sine vibration experiments of the real system show a maximal inaccuracy of 8. 8 %. Application of this model and procedure can simplify the modeling and analysis of mechanical systems including rubber materials.
基金supported by the National Natural Science Foundation of China(Grant No.51178406)the Research Funding from the State Key Laboratory for Disaster Reduction in Civil Engineering at Tongji University(Grant No.SLDRCE10-MB-01)the Fujian Natural Science Foundation Project(Grant No.2010J01309)
文摘Seismic behaviors of base-isolated structures are highly affected by the nonlinear characteristics of the isolated systems. Most of the currently available methods for the identification of nonlinear properties of isolator require either the measurements of all structural responses or the assumptions of the proper mathematic models for the rubber-bearings. In this paper, two algorithms are proposed to identify the nonlinear properties of rubber-bearings in base-isolated buildings using only partial measurements of structural dynamic responses. The first algorithm is applicable to the case that proper mathematical models are available for the base isolators. It is based on the extended Kalman filter for the parametric identification of nonlinear models of rubber-bearing isolators and buildings. For the general case where it is difficult to establish a proper mathematical model to describe the nonlinear behavior of a rubber-bearing isolator, another algorithm is proposed to identify the model-tYee nonlinear property of rubber-bearing isolated system. Nonlinear effect of rubber-bearing is treated as ＇fictitious loading＇ on the linear building under severe earthquake. The algorithm is based on the sequential Kalman estimator for the structural responses and the least-squares estimation of the ＇fictitious loading＇ to identify the nonlinear force of rubber-bearing isolator. Simulation results demonstrate that the proposed two algorithms are capable of identifying the nonlinear properties of rubber-bearing isolated systems with good accuracy.
