An HPLC-DAD-ELSD method was developed and validated for the simultaneous quantitation of seven active components（liquiritin, baicalin, wogonoside, baicalein, wogonin, ginsenosides Re and ginsenosides Rb1） in n-butan...An HPLC-DAD-ELSD method was developed and validated for the simultaneous quantitation of seven active components（liquiritin, baicalin, wogonoside, baicalein, wogonin, ginsenosides Re and ginsenosides Rb1） in n-butanol effective fraction of Xiao Chai Hu Tang. A Diamonsil C18（2） column（4.6 mm × 250 mm, 5 μm） was used as the stationary phase and the mobile phase was consisted of acetonitrile and aqueous phosphate acid（0.05%, v/v）. Gradient elution was carried out at the flow rate of 1 m L/min. The detection wavelength was set at 276 nm and an evaporative light scattering detector was also used. Good linearity for all the seven active components was observed. The established method is simple, fast, reliable, and suitable for the quality control of n-butanol effective fraction of Xiao Chai Hu Tang.展开更多
基金Foundation for Young Scholars of Shanxi Province,China(Grant No.2011021007-2)
文摘An HPLC-DAD-ELSD method was developed and validated for the simultaneous quantitation of seven active components（liquiritin, baicalin, wogonoside, baicalein, wogonin, ginsenosides Re and ginsenosides Rb1） in n-butanol effective fraction of Xiao Chai Hu Tang. A Diamonsil C18（2） column（4.6 mm × 250 mm, 5 μm） was used as the stationary phase and the mobile phase was consisted of acetonitrile and aqueous phosphate acid（0.05%, v/v）. Gradient elution was carried out at the flow rate of 1 m L/min. The detection wavelength was set at 276 nm and an evaporative light scattering detector was also used. Good linearity for all the seven active components was observed. The established method is simple, fast, reliable, and suitable for the quality control of n-butanol effective fraction of Xiao Chai Hu Tang.
