微孔注塑数值模拟的正交化试验与分析

构建了微孔注塑充模过程的数学模型,并利用Moldflow Plastics Insight 6.1进行成型窗口模拟。在注射量、注射时间、熔体温度、模具温度四个工艺参数的可行区间内,分别确定三个值,构建正交化模拟试验并分别求解,利用信噪比极差法确定最优加工参数组合。结果表明:注射量是影响泡孔半径的主要因素,较高的注射量可以得到更加均匀细密的泡孔结构。

基于正交试验法的聚酰亚胺复合材料注塑成型工艺优化试验

提出应用注射成型工艺方法,通过正交试验优化设计开展了工艺参数优化试验,研究了各注塑工艺参数对于最终成品质量的影响,找到了最优的工艺参数,并通过生产试验,成功减小了零件翘曲变形量,实现了产品的直接注塑成型,提高了生产效率和成品率。

密肋复合墙板框格单元的有限元分析与设计优化

针对密肋复合墙板框格单元设计优化问题,采用框格单元的正交化承载试验模拟方法,编制了有限元程序,分析了框格单元顶点位移的影响因素和填充材料中心节点应力状态的影响因素,并提出了对填充材料性能指标和框格单元尺寸的建议性取值范围.

Process parameters optimization of Ti-6Al-4V alloy sheet during hot stretch-creep forming

Hot stretch-creep forming （SCF） is a novel technique to produce hard-to-form thin-walled metal components. Comprehensively considering the analysis results of the springback angle, yield strength and microstructure, four hot SCF process parameters including temperature, stretch velocity, post stretch percentage and dwelling time of a Ti-6Al-4V alloy sheet were optimized using an orthogonal experiment. The results reveal that temperature is the most important factor on springback angle. The yield strength of the deformed material in 0° direction increases, while those in directions of 45° and 90° fluctuate around the original value. After hot SCF, the shape of some a phases changes from short thin grains to long slender ones, and the microhardness changes very little. The optimized parameters with temperature of 700 ℃, stretch velocity of 5 mm/min, post stretch percentage of 2% and dwelling time of 8 min are achieved finally.

Study on Effects of Different Factors to Eggs Hatching Rate of Cryptotympana atrata by Orthogonal Design

[Objective] The paper aimed to explore influence of different factors on eggs hatching of Cryptotympana atrata,and optimize technical parameters in practice.[Method] By using hatching rate as index,L16（44）orthogonal design was employed to determine the effects of the four factors including temperature,salinity,photoperiod and pH.[Result] Photoperiod had obviously effects on hatchability of Cryptotympana atrata eggs.The optimal conditions for hatching were temperature 30 ℃,salinity 25%,photoperiod L12∶D12,and pH 6.[Conclusion]The research provided some scientific basis for indoor artificial incubation.

扭转条件下阶梯轴退刀槽结构的有限元分析

退刀槽结构易引起轴类零件产生应力集中。首先建立了阶梯轴退刀槽的有限元分析模型,并分别对纯矩形、带圆弧的矩形和1/4椭圆圆弧的退刀槽在扭矩条件下进行了分析,在满足工艺需求的前提下,对于纯矩形退刀槽应优先考虑增大其宽度,对于1/4椭圆圆弧过渡的退刀槽,其椭圆长短轴之比应控制在1.5倍;同时通过对带圆弧矩形退刀槽参数的正交化试验,确立了降低其应力集中系数因素的主次关系为退刀槽宽度、退刀槽深度和其圆弧半径,所得结果可为退刀槽的设计与制造提供指导。

Optimization of PCR Reaction System for Random Single-strand DNA Pool in SELEX Technology

[Objective] This study aimed to optimize the PCR amplification conditions for random ssDNA pool in SELEX technology. [Method] L16（45） orthogonal experimental design was adopted for optimization of five important factors affecting PCR reaction system for random single-stranded DNA pool including Mg2＋ concentration, dNTP concentration, amount of Taq DNA polymerase, primer concentration and amount of random single-stranded DNA pool at four levels. Meanwhile, the annealing temperature and number of PCR reaction cycles were optimized to establish the optimal reaction system and PCR procedure. [Result] The optimal combination of PCR reaction system for random ssDNA pool was obtained, with a total system volume of 20 μl containing 2.0 μl of 10 × Buffer, 0.5 ng of random ssDNA pool, 2.5 mmol/L Mg2＋, 0.25 mmol/L dNTP Mixture, 0.6 μmol/L upstream and downstream primers and 1.5 U of Taq DNA polymerase; the optimal annealing temperature was 68 ℃ and the optimal number of cycles was 12. Under the above conditions, clear and stable bands with high specificity for random ssDNA pool were amplified. [Conclusion] This study laid the foundation for selection of parameters with higher specificity in SELEX technology.

Optimization of RAPD-PCR Reaction System for Glycyrrhiza uralensis Based on Orthogonal Design

[Objective] The aim was to obtain the optimum RAPD-PCR reaction system for Glycyrrhiza uralensis.[Method] Orthogonal design was adopted to screen the suitable concentration of four major factors（dNTPs,primers,Taq polymerase and DNA template） in PCR reaction system.[Result] The optimal reaction system obtained by orthogonal design was 25 μl in total volume,containing 2.5 μl of 10×PCR buffer solution（include MgCl2）,2.5 μl of 10 mmol/L dNTPs,2 μl（100 ng） of DNA template,2 μl of 10 μmol/L primers,0.4 μl（5 U） of Taq polymerase;the optimum annealing temperature was 34 ℃.[Conclusion] Orthogonal design was an effective method for the optimization of RAPD-PCR reaction system for G.uralensis.

Extraction of Perilla Seed Oil Using Supercritical Carbon Dioxide and GC-MS Analysis

[Objective] This study was aimed to determine the optimal parameters for the extraction of perilla seed oil to obtain high-quality perilla seed oil and analyze its compositions. [Method] In this study, perilla seed oil was extracted using supercritical CO2 （SC-CO2）. The effects of extraction time, temperature and pressure were investigated by single-factor experiments and orthogonal array testing （ORT）. The chemical compositions of extracted perilla seed oil were investigated by GC-MS. [Result] The optimal conditions for the extraction of perilla seed oil using SC-CO2 were extraction time of 4 h, extraction temperature at 40 ℃, and extraction pressure at 23 MPa. Under these conditions, the extraction yield of perilla seed oil was maximized to 12.43%. GC-MS analysis revealed that perilla seed oil was a complex mixture containing 76.183% α-linolenic acid. [Conclusion] Supercritical CO2 extraction was proven to be an effective technology to extract oil from perilla seed, and GCMS was also a satisfactory method for analyzing the compositions of perilla seed oil.

Numerical simulation of intelligent compaction for subgrade construction

During the compaction of a road subgrade, the mechanical parameters of the soil mass change in real time, but current research assumes that these parameters remain unchanged. In order to address this discrepancy, this paper establishes a relationship between the degree of compaction K and strain ε. The relationship between the compaction degree K and the shear strength of soil(cohesion c and frictional angle φ) was clearly established through indoor experiments. The subroutine UMAT in ABAQUS finite element numerical software was developed to realize an accurate calculation of the subgrade soil compaction quality. This value was compared and analyzed against the assumed compaction value of the model, thereby verifying the accuracy of the intelligent compaction calculation results for subgrade soil. On this basis, orthogonal tests of the influential factors(frequency, amplitude, and quality) for the degree of compaction and sensitivity analysis were carried out. Finally, the ‘acceleration intelligent compaction value’, which is based on the acceleration signal, is proposed for a compaction meter value that indicates poor accuracy. The research results can provide guidance and basis for further research into the accurate control of compaction quality for roadbeds and pavements.

Damage evolution analysis of cast steel GS-20Mn5V based on modified GTN model

A modified Gurson-Tvergaard-Needleman （GTN） model that accounts for the mixed （isotropic and kinematic） hardening of cast steel GS-20Mn5V was developed and implemented in the finite dement program ABAQUS/Standard via a user-defined material subroutine UMAT. This model couples the stress state and damage evolution （pore volume fraction increase） by a classic method that assumes that the total void volume fraction is divided into a nucleation and a growth part. A parametric study was conducted to assess the effect of modified GTN model parameters on mechanical properties such as the nucleation, growth and coalescence of voids and to obtain the optimal parameter combination by the orthogonal test method. The predicted load-displacement curves of notched specimens with the optimal parameters are favorably compared to the experimental curves. Therefore, the modified GTN model can be used to predict the damage evaluation and fracture behavior of GS-20Mn5V.

Application of orthogonal experimental design and Tikhonov regularization method for the identification of parameters in the casting solidification process

The inverse heat conduction method is one of methods to identify the casting simulation parameters. A new inverse method was presented according to the Tikhonov regularization theory. One appropriate regularized functional was established, and the functional was solved by the sensitivity coefficient and Newtonaphson iteration method. Moreover, the orthogonal experimental design was used to estimate the appropriate initial value and variation domain of each variable to decrease the number of iteration and improve the identification accuracy and efficiency. It illustrated a detailed case of AlSiTMg sand mold casting and the temperature measurement experiment was done. The physical properties of sand mold and the interracial heat transfer coefficient were identified at the meantime. The results indicated that the new regularization method was efficient in overcoming the ill-posedness of the inverse heat conduction problem and improving the stability and accuracy of the solutions.

Numerical simulation study of the failure evolution process and failure mode of surrounding rock in deep soft rock roadways

Based on the safety coefficient method,which assigns rock failure criteria to calculate the rock mass unit,the safety coefficient contour of surrounding rock is plotted to judge the distribution form of the fractured zone in the roadway.This will provide the basis numerical simulation to calculate the surrounding rock fractured zone in a roadway.Using the single factor and multi-factor orthogonal test method,the evolution law of roadway surrounding rock displacements,plastic zone and stress distribution under different conditions is studied.It reveals the roadway surrounding rock burst evolution process,and obtains five kinds of failure modes in deep soft rock roadway.Using the fuzzy mathematics clustering analysis method,the deep soft surrounding rock failure model in Zhujixi mine can be classified and patterns recognized.Compared to the identification results and the results detected by geological radar of surrounding rock loose circle,the reliability of the results of the pattern recognition is verified and lays the foundations for the support design of deep soft rock roadways.

Optimization of IBRS-2 Cells Culturing Conditions for Cultivation of Porcine Parvovirus N Strain Using Roller Bottle

The aim was to optimize the roller bottle culture system of IBRS-2 cells for cultivation of porcine parvovirus （PPV） N strain using a four-factor three-level or- thogonal test （cell culture medium pH value： 7.0, 7.2 and 7.4; inoculation time： 0, 24 and 48 h; inoculation dose： 0.10%, 1.00% and 10.00%; harvest time： 48, 72 and 96 h）. The results showed that the optimal conditions were as follows： cell culture medium pH value of 7.2, synchronous inoculation, inoculation dose of 0.10% and culture time of 96 h. Among the four factors, culture time was the most important factor affectina the virulence titer of PPV N strain.

Purification of Crude Glycerol from Waste Cooking Oil Based Biodiesel Production by Orthogonal Test Method

Purification of original crude glycerol obtained from biodiesel production was conducted in a laboratory scale equipment by means of a combined chemical and physical treatment method based upon repeated cycles of acidification of liquid phase to the desired pH value by using 5.85% H3PO4 solution for pH value adjustment, and the mixture was kept at 70 ℃ for 60 rain to make phase separation for obtaining a glycerol-rich middle phase. The yield of crude glycerol reached 81.2%. Subsequently, upon reaction of the obtained glycerol phase with 0.03% of sodium oxalate at 80 ℃ for 30 min the impurity removal rate was equal to 19.8%. The fraction boiling between 164 ℃ and 200 ℃ was collected by vacuum distil- lation followed by decolorization with 2% of active carbon at 80 ℃ for two times to yield the product glycerol with an ac- ceptable purity of 98.10%.

Process Optimization of Ultrasonic Extraction of Puerarin Based on Support Vector Machine

In ultrasonic extraction technology, optimization of technical parameters often considers extraction medium only, without including ultrasonic parameters. This paper focuses on controlling the ultrasonic extraction process of puerarin, investigating the influence of ultrasonic parameters on extraction rate, and empirically analyzing the main components of Pueraria, i.e., isoflavone compounds. A method is presented combining orthogonal experi- mental design with a support vector machine and a predictive model is established for optimization of technical parameters. From the analysis with the predictive model, appropriate process parameters are achieved for higher extraction rate. With these parameters in the ultrasonic extraction of puerarin, the experimental result is satisfactory. This method is of significance to the study of extracfing root-stock plant medicines.

Optimization Design of Magnetic Field on Cold Yoke Superconducting Solenoid

The optimized design of magnetic field for a cold yoke superconducting solenoid is introduced in this paper. Using some kinds of optimization designs and OPERA,we optimize the main solenoid,cold yoke and compensated winding.Through this design,the requests of the superconducting solenoid are realized.